Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,976,793 C→A 14.0% H153N (CAC→AAC)  PP_1770 → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,976,7930CA14.0% 156.2 / 15.2 57H153N (CAC→AAC) PP_17703‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base A (4/4);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

TCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGCCA  >  NC_002947/1976653‑1976939
                                                                                                                                            |                                                                                                                                                  
tCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtg                                                                                                                                            <  1:18286/149‑2 (MQ=255)
tCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtg                                                                                                                                            >  1:236249/1‑148 (MQ=255)
     aGCGCCAAGGGCAAGGTGGTGCGTGAGGCGCGTGCCGTCTTCGGTTCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtggcatt                                                                                                                                       <  2:236249/149‑7 (MQ=255)
     aGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCt                                                                                                                                       <  6:289164/149‑1 (MQ=255)
                  aCGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGcc                                                                                                                          <  6:296039/149‑1 (MQ=255)
                    gtcgtGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCg                                                                                                                         >  2:142102/1‑148 (MQ=255)
                               ggCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCccg                                                                                                             >  8:109417/1‑149 (MQ=255)
                                gcgcGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCccgc                                                                                                            <  7:108294/149‑1 (MQ=255)
                                gcgcGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTACCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGACACCTTGTTCCGCCGGCACAAGGTAATCCTCACGCCGCTGGCCGAAAACGACCccgc                                                                                                            >  5:163536/1‑149 (MQ=255)
                                       ccGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACCAGGTCATCCTCACGCCGCTGGCCGAAACCGCCCCCGCCGCGCTg                                                                                                     >  3:25016/1‑149 (MQ=255)
                                            ttCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCt                                                                                                >  7:126139/1‑149 (MQ=255)
                                             tCGGTGCCCGCCTGCCGCGCTTCGTCCCGTGCCACCCAATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCt                                                                                                <  6:163536/148‑1 (MQ=255)
                                              cGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAAc                                                                                                                     <  8:87981/126‑1 (MQ=255)
                                              cGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAAc                                                                                                                     >  7:87981/1‑126 (MQ=255)
                                               ggTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACATGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTgg                                                                                              <  1:284261/148‑1 (MQ=255)
                                                gTGCCCGCCTGCCGCGCTTCGTCCCGGGCCAGCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGtt                                                                                            <  7:245339/149‑1 (MQ=255)
                                                   cccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAc                                                                                         <  1:142102/149‑1 (MQ=255)
                                                    ccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAcc                                                                                        >  6:217296/1‑149 (MQ=255)
                                                    ccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTAGTTCCGCCGGCACAAGGTCATCCTCCCGCCGCTGGCCGAACCCGCCCCCGCCGCGCTCGTCCTGGTTGAcc                                                                                        >  7:205726/1‑149 (MQ=255)
                                                     cGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCg                                                                                       <  2:164218/149‑1 (MQ=255)
                                                       ccTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGcc                                                                                     >  3:120955/1‑149 (MQ=255)
                                                            cgcgCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTgg                                                                                >  2:30623/1‑149 (MQ=255)
                                                             gcgcTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGc                                                                               <  8:354014/149‑1 (MQ=255)
                                                             gcgcTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGc                                                                               <  4:131951/149‑1 (MQ=255)
                                                                    gTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCt                                                                        <  8:119837/149‑1 (MQ=255)
                                                                       ccGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGa                                                                     >  2:25046/1‑149 (MQ=255)
                                                                           gCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGcc                                                                 >  5:6008/1‑149 (MQ=255)
                                                                              aCCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGAt                                                              >  6:357077/1‑149 (MQ=255)
                                                                                cccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGAtgt                                                            >  4:363744/1‑149 (MQ=255)
                                                                                cccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAg                                                                                                                                               <  4:196634/66‑1 (MQ=38)
                                                                                cccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAg                                                                                                                                               >  3:196634/1‑66 (MQ=38)
                                                                                   aTCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGAtgtg                                                           >  7:173066/1‑147 (MQ=255)
                                                                                   aTCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGa                                                         <  6:320435/149‑1 (MQ=255)
                                                                                    tCGCCGGTTCCGCGCAGAGCGGGGTTGAGGCGTCCAATGCCACCTTGTTCGGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGATACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAg                                                        <  8:205726/149‑1 (MQ=255)
                                                                                        cGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGcaca                                                    >  6:302594/1‑149 (MQ=255)
                                                                                         ggTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATc                                                                                                                                         <  4:304673/63‑1 (MQ=255)
                                                                                         ggTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATc                                                                                                                                         >  3:304673/1‑63 (MQ=255)
                                                                                         ggTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACAt                                                   <  3:363744/149‑1 (MQ=255)
                                                                                          gTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATg                                                  <  4:105113/149‑1 (MQ=255)
                                                                                              cGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTgg                                                                                                    <  6:121522/95‑1 (MQ=255)
                                                                                              cGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTgg                                                                                                    >  5:121522/1‑95 (MQ=255)
                                                                                              cGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcg                                               <  5:302594/148‑1 (MQ=255)
                                                                                               gAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggt                                             >  3:52241/1‑149 (MQ=255)
                                                                                               gAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggt                                             <  7:109417/149‑1 (MQ=255)
                                                                                               gAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggt                                             >  7:184902/1‑149 (MQ=255)
                                                                                                  cAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggtc                                            >  7:283664/1‑147 (MQ=255)
                                                                                                     agCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgcg                                       <  8:126139/149‑1 (MQ=255)
                                                                                                                    tCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACt                        >  5:54530/1‑149 (MQ=255)
                                                                                                                    tCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACt                        <  8:283664/149‑1 (MQ=255)
                                                                                                                      cAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTg                       <  6:6008/148‑1 (MQ=255)
                                                                                                                         tGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTg                       <  4:353967/145‑1 (MQ=255)
                                                                                                                         tGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTg                       >  3:353967/1‑145 (MQ=255)
                                                                                                                              ccTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGccac               >  2:33000/1‑148 (MQ=255)
                                                                                                                                      cggcgGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCTTGACGAAGTACTGGCCGCCACCAGCCATCt      <  1:33000/146‑1 (MQ=255)
                                                                                                                                        gcgGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGc    >  4:156878/2‑149 (MQ=255)
                                                                                                                                        ccgGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGc    <  1:150838/149‑1 (MQ=255)
                                                                                                                                           gCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGCca  >  3:168757/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGCCA  >  NC_002947/1976653‑1976939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑