Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,991,155	T→G	7.5%	L802* (TTA→TGA)	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,991,155	0	T	G	7.5%	131.7 / 2.5	50	L802* (TTA→TGA)	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base G (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

AACCGGACAAGCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGAT  >  NC_002947/1991008‑1991297                                                                                                                                                    |                                                                                                                                               aaCCGGACAAGCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTa                                                                                                                                               <  6:263546/149‑1 (MQ=255) aaCCGGACAAGCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTa                                                                                                                                               <  5:271180/149‑1 (MQ=255)  aCCGGACAAGCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAg                                                                                                                                              >  6:131870/1‑149 (MQ=255)       aCAAGCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGt                                                                                                                                         <  7:30558/149‑1 (MQ=255)           gCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGcc                                                                                                                                      >  8:254948/1‑148 (MQ=255)           gCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGcc                                                                                                                                      >  6:186856/1‑148 (MQ=255)           gCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGAGCAGGCGGTTTTAGCCAGTGcc                                                                                                                                      >  4:136199/1‑148 (MQ=255)            cATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCaa                                                                                                                                    <  3:23497/149‑1 (MQ=255)                 cTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGc                                                                                                                               >  4:144059/1‑149 (MQ=255)                      cccTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCaaa                                                                                                                          <  6:124497/149‑1 (MQ=255)                         tCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAacac                                                                                                                       >  7:195681/1‑149 (MQ=255)                         tCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAacac                                                                                                                       >  6:377963/1‑149 (MQ=255)                             gATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGaaa                                                                                                                   <  7:254948/149‑1 (MQ=255)                                 ggCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGcc                                                                                                               <  5:186856/149‑1 (MQ=255)                                  gCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCcg                                                                                                              >  1:106668/1‑149 (MQ=255)                                          cgcgGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGgcc                                                                                                        <  5:157698/147‑1 (MQ=255)                                           gcgGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGgccgcc                                                                                                     <  2:331210/149‑1 (MQ=255)                                                       tcgAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTGAGCCAGTTGGCACTGGCTAAAaccgcct                                                                                                                    <  3:174375/122‑6 (MQ=38)                                                       tcgAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTGAGCCAGTTGGCACTGGCTAAAaccgcct                                                                                                                    >  4:174375/1‑117 (MQ=38)                                                        cgAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACATGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGAcc                                                                                         >  1:272568/1‑148 (MQ=255)                                                         gAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCa                                                                                        >  6:21664/1‑148 (MQ=255)                                                                     gCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTggtggtggt                                                                           >  6:103063/1‑149 (MQ=255)                                                                     gCACTCGAGCAGCCCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGTGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTggtggtggt                                                                           >  3:373306/1‑149 (MQ=255)                                                                        cTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAAc                                                                                     >  6:147300/1‑136 (MQ=255)                                                                        cTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAAc                                                                                     <  5:147300/136‑1 (MQ=255)                                                                           gAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTtctc                                                                      <  2:106668/148‑1 (MQ=255)                                                                              cagcGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTg                                                                  >  5:282191/1‑149 (MQ=255)                                                                              cagcGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTg                                                                  <  2:131971/149‑1 (MQ=255)                                                                               agcGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTgg                                                                 <  8:195681/149‑1 (MQ=255)                                                                                          cAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATg                                                      >  2:317540/1‑149 (MQ=255)                                                                                           aaGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGc                                                     >  7:317206/1‑149 (MQ=255)                                                                                                aTTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCAt                                                >  2:333904/1‑149 (MQ=255)                                                                                                    aaCTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCt                                            <  6:282191/149‑1 (MQ=255)                                                                                                      cTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTgg                                                                 <  6:151100/126‑1 (MQ=255)                                                                                                      cTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGa                                          <  1:333904/149‑1 (MQ=255)                                                                                                              cagcaTCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTc                                  <  1:342328/149‑1 (MQ=255)                                                                                                                gcaTCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAg                                <  8:317206/149‑1 (MQ=255)                                                                                                                gcaTCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAg                                <  5:21664/149‑1 (MQ=255)                                                                                                                   tCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCa                             >  3:183525/1‑149 (MQ=255)                                                                                                                      acacTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTg                          >  5:137505/1‑149 (MQ=255)                                                                                                                          ttGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAAAACGAAATGCAGGAGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACAATCAGGCATTGCt                        >  1:46334/1‑147 (MQ=255)                                                                                                                              gAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGa                  <  5:103063/149‑1 (MQ=255)                                                                                                                               aGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAg                 >  1:7532/1‑149 (MQ=255)                                                                                                                                    agTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCaa            <  1:317540/149‑1 (MQ=255)                                                                                                                                           aGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGAc     <  3:144059/149‑1 (MQ=255)                                                                                                                                            ggCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCa                      >  6:254593/1‑131 (MQ=255)                                                                                                                                            ggCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCa                      <  5:254593/131‑1 (MQ=255)                                                                                                                                               tgTTTGAGCCAGTTGGCACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATaca                                     >  3:354136/2‑113 (MQ=255)                                                                                                                                               tgTTTGAGCCAGTTGGCACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATaca                                     <  4:354136/112‑1 (MQ=255)                                                                                                                                               ggTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGGTCAGTGAGTTCAAGCAGGACGAt  >  2:118406/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                               AACCGGACAAGCATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGAT  >  NC_002947/1991008‑1991297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑