Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,991,166	A→C	9.1%	N806H (AAC→CAC)	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,991,166	0	A	C	9.1%	129.3 / 5.3	44	N806H (AAC→CAC)	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (21/19);  new base C (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

CATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGATTACGACGTTCGCATCT  >  NC_002947/1991019‑1991313                                                                                                                                                    |                                                                                                                                                    cATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCaa                                                                                                                                                    <  3:23497/149‑1 (MQ=255)      cTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGc                                                                                                                                               >  4:144059/1‑149 (MQ=255)           cccTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCaaa                                                                                                                                          <  6:124497/149‑1 (MQ=255)              tCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAacac                                                                                                                                       >  6:377963/1‑149 (MQ=255)              tCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAacac                                                                                                                                       >  7:195681/1‑149 (MQ=255)                  gATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGaaa                                                                                                                                   <  7:254948/149‑1 (MQ=255)                      ggCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGcc                                                                                                                               <  5:186856/149‑1 (MQ=255)                       gCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCcg                                                                                                                              >  1:106668/1‑149 (MQ=255)                               cgcgGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGgcc                                                                                                                        <  5:157698/147‑1 (MQ=255)                                gcgGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGgccgcc                                                                                                                     <  2:331210/149‑1 (MQ=255)                                            tcgAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTGAGCCAGTTGGCACTGGCTAAAaccgcct                                                                                                                                    >  4:174375/1‑117 (MQ=38)                                            tcgAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTGAGCCAGTTGGCACTGGCTAAAaccgcct                                                                                                                                    <  3:174375/122‑6 (MQ=38)                                             cgAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACATGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGAcc                                                                                                         >  1:272568/1‑148 (MQ=255)                                              gAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCa                                                                                                        >  6:21664/1‑148 (MQ=255)                                                          gCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTggtggtggt                                                                                           >  6:103063/1‑149 (MQ=255)                                                          gCACTCGAGCAGCCCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGTGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTggtggtggt                                                                                           >  3:373306/1‑149 (MQ=255)                                                             cTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAAc                                                                                                     >  6:147300/1‑136 (MQ=255)                                                             cTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAAc                                                                                                     <  5:147300/136‑1 (MQ=255)                                                                gAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTtctc                                                                                      <  2:106668/148‑1 (MQ=255)                                                                   cagcGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTg                                                                                  <  2:131971/149‑1 (MQ=255)                                                                   cagcGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTg                                                                                  >  5:282191/1‑149 (MQ=255)                                                                    agcGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTgg                                                                                 <  8:195681/149‑1 (MQ=255)                                                                               cAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATg                                                                      >  2:317540/1‑149 (MQ=255)                                                                                aaGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGc                                                                     >  7:317206/1‑149 (MQ=255)                                                                                     aTTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCAt                                                                >  2:333904/1‑149 (MQ=255)                                                                                         aaCTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCt                                                            <  6:282191/149‑1 (MQ=255)                                                                                           cTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTgg                                                                                 <  6:151100/126‑1 (MQ=255)                                                                                           cTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGa                                                          <  1:333904/149‑1 (MQ=255)                                                                                                   cagcaTCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTc                                                  <  1:342328/149‑1 (MQ=255)                                                                                                     gcaTCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAg                                                <  8:317206/149‑1 (MQ=255)                                                                                                     gcaTCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAg                                                <  5:21664/149‑1 (MQ=255)                                                                                                        tCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCa                                             >  3:183525/1‑149 (MQ=255)                                                                                                           acacTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTg                                          >  5:137505/1‑149 (MQ=255)                                                                                                               ttGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAAAACGAAATGCAGGAGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACAATCAGGCATTGCt                                        >  1:46334/1‑147 (MQ=255)                                                                                                                   gAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGa                                  <  5:103063/149‑1 (MQ=255)                                                                                                                    aGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAg                                 >  1:7532/1‑149 (MQ=255)                                                                                                                         agTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCaa                            <  1:317540/149‑1 (MQ=255)                                                                                                                                aGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGAc                     <  3:144059/149‑1 (MQ=255)                                                                                                                                 ggCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCa                                      <  5:254593/131‑1 (MQ=255)                                                                                                                                 ggCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCa                                      >  6:254593/1‑131 (MQ=255)                                                                                                                                    tgTTTGAGCCAGTTGGCACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATaca                                                     <  4:354136/112‑1 (MQ=255)                                                                                                                                    tgTTTGAGCCAGTTGGCACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATaca                                                     >  3:354136/2‑113 (MQ=255)                                                                                                                                    ggTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGGTCAGTGAGTTCAAGCAGGACGAt                  >  2:118406/1‑148 (MQ=255)                                                                                                                                          aGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGATTacgacg           >  1:157925/1‑149 (MQ=255)                                                                                                                                               gTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGATTACGACGTTCg       >  1:378738/1‑148 (MQ=255)                                                                                                                                                    aaCTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGATTACGACGTTCGCATCt  >  3:323721/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CATACCTTGGCCCTCAAGATGGGCGAACTGCGCGGCAAAATCGTCGAAGGCGACGATGCACTCGAGCAGCGCCGGCGCCAAGGCATTGAACTTGTTCGCAGCATCTACACTTGGGAGCAGAGTGCGCAGGCGGTTTTAGCCAGTGCCAACTGGCTCAAACACGAAATGCCGGCGGCCGCCGCCAGCAAGACCAAACTGGTGGTGGTTTCTCCTTGGGCAACCAAATGCGGCATTGCTGAATACACTCAGGCATTGCTCAGTGAGTTCAAGCAGGACGATTACGACGTTCGCATCT  >  NC_002947/1991019‑1991313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑