Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,062,851	G→A	7.7%	C589Y (TGC→TAC) ‡	PP_1838 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,062,851	0	G	A	7.7%	187.0 / 4.1	65	C589Y (TGC→TAC) ‡	PP_1838	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/33);  new base A (2/3);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-01

CTGGACCTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATGTACCGCTA  >  NC_002947/2062703‑2062998                                                                                                                                                     |                                                                                                                                                    cTGGACCTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTg                                                                                                                                                     >  6:309810/1‑149 (MQ=255)  tGGACCTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGc                                                                                                                                                    >  2:300532/1‑149 (MQ=255)     aCCTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTgg                                                                                                                                                 <  5:309810/149‑1 (MQ=255)      ccTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTggg                                                                                                                                                <  8:123489/149‑1 (MQ=255)       cTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTAGGTGGCAAGGCTCGCCACCAGTGCTggg                                                                                                                                                <  6:348353/148‑1 (MQ=255)          ggCCGGTTCAACGTGGCGCTGGTGCTGATTGCCCCGGGCATTGAGGAGAAGTTCGGGTCGGTGTATCACACCGGGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCCCCAGCACTGGTGgcg                                                                                                                                            <  3:37191/149‑1 (MQ=255)              gCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGAc                                                                                                                                        <  6:176557/149‑1 (MQ=255)                    aCGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTc                                                                                                                                  <  6:23317/149‑1 (MQ=255)                     cGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTc                                                                                                                                  >  7:214111/1‑148 (MQ=255)                           gctgctgctGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCACCCTGc                                                                                                                           >  6:335471/1‑149 (MQ=255)                               ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCGCGCCCCCAGCACTGGTGgcgagcctt                                                                                                                                      >  2:237640/1‑128 (MQ=255)                               ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCGCGCCCCCAGCACTGGTGgcgagcctt                                                                                                                                      <  1:237640/134‑7 (MQ=255)                                  ctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAgg                                                                                                                     <  3:293681/148‑1 (MQ=255)                                  ctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAgg                                                                                                                     <  4:156314/148‑1 (MQ=255)                                   tgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGa                                                                                                                       >  7:313541/1‑145 (MQ=255)                                   tgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGa                                                                                                                       <  8:313541/145‑1 (MQ=255)                                     aTTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGa                                                                                                                 >  6:14492/1‑149 (MQ=255)                                     aTTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGa                                                                                                                 >  5:355925/1‑149 (MQ=255)                                     aTTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGa                                                                                                                 >  4:313966/1‑149 (MQ=255)                                          cccGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCCCCAGCACTGGTGgcga                                                                                                                                           >  7:55730/1‑117 (MQ=255)                                          cccGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCCCCAGCACTGGTGgcga                                                                                                                                           <  8:55730/118‑2 (MQ=255)                                                cATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAg                                                                                                      <  6:194652/149‑1 (MQ=255)                                                  ttCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGc                                                                                                    <  2:203473/149‑1 (MQ=255)                                                     aGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCAt                                                                                                  >  6:36076/1‑148 (MQ=255)                                                       gagaAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGAt                                                                                               >  1:33355/1‑149 (MQ=255)                                                          aaGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCaa                                                                                            <  1:300532/149‑1 (MQ=255)                                                           aGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAg                                                                                           <  3:313966/149‑1 (MQ=255)                                                                     ggtgAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCggg                                                                                   >  7:35304/1‑147 (MQ=255)                                                                     ggtgAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCa                                                                                 <  4:304589/149‑1 (MQ=255)                                                                      gtgAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAg                                                                                >  4:192109/1‑149 (MQ=255)                                                                           tCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAg                                                                            >  7:336405/1‑148 (MQ=255)                                                                           tCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAg                                                                            >  8:313521/1‑148 (MQ=255)                                                                                   tGGGTACCCAGGTCGACATCGTTCGGAGCATCATGGGCCGCCTGGGTGGCAAGGCTGGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTc                                                                   <  6:299902/149‑1 (MQ=255)                                                                                   tGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTc                                                                   <  3:72515/149‑1 (MQ=255)                                                                                     ggTACCCAGGTCGACATCGTGCCGACCATCCTGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCgg                                                                 <  5:14492/149‑1 (MQ=255)                                                                                     ggTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCgg                                                                 <  2:188793/149‑1 (MQ=255)                                                                                         cccAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTg                                                             <  2:355423/149‑1 (MQ=255)                                                                                          ccAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTgg                                                            >  4:75690/1‑149 (MQ=255)                                                                                          ccAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATAAAGCCGTCGGGCAGCGAGCAGATTGTCGGCCTgg                                                            >  8:342727/1‑149 (MQ=255)                                                                                            aGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTg                                                          >  5:222567/1‑149 (MQ=255)                                                                                              gTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTg                                                          >  6:245133/1‑147 (MQ=255)                                                                                               tCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAg                                                       <  5:36076/149‑1 (MQ=255)                                                                                                 gACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAgg                                                      <  8:214111/148‑1 (MQ=255)                                                                                                     tCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGAc                                                 <  3:192109/149‑1 (MQ=255)                                                                                                     tCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGAc                                                 <  6:355925/149‑1 (MQ=255)                                                                                                      cGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCAGGAGCAGGGTGAcc                                                >  6:209556/1‑149 (MQ=255)                                                                                                       gTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTTGGGGCGCGACCTGCTCAACCTTCCCGAGGGTGACCAGGGCGTAGGCATGAACAAGCCGTCGGGCAGCGTGCTGATTGTCGGGCTGGTGCAGGGTGAcc                                                <  8:299287/148‑1 (MQ=255)                                                                                                        tGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGt                                                                    <  2:253189/127‑1 (MQ=255)                                                                                                        tGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGt                                                                    >  1:253189/1‑127 (MQ=255)                                                                                                         gCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTa                                             >  2:290274/1‑149 (MQ=255)                                                                                                          ccGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTa                                             <  1:290274/148‑1 (MQ=255)                                                                                                               catcatGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTAt                                            >  4:289765/1‑144 (MQ=255)                                                                                                               catcatGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTAt                                            <  3:289765/144‑1 (MQ=255)                                                                                                                   atGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATc                                     >  6:114173/1‑147 (MQ=255)                                                                                                                       gcctgcTGGGTGGCAAGGCTCGCCACGAGTGCTGGGGGCGCGACCTTCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCTGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTcc                               <  7:342727/144‑1 (MQ=255)                                                                                                                           ccTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAgg                           >  3:342041/1‑149 (MQ=255)                                                                                                                            cTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGa                          <  7:313521/149‑1 (MQ=255)                                                                                                                            cTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGa                          >  7:220249/1‑149 (MQ=255)                                                                                                                              gggTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGCCCGTAACCTGATCGAGTCCAAGGACa                        >  3:264627/1‑149 (MQ=255)                                                                                                                               ggTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGAc                         <  8:40367/147‑1 (MQ=255)                                                                                                                               ggTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACAt                       <  5:114173/149‑1 (MQ=255)                                                                                                                               ggTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACAt                       <  8:35304/149‑1 (MQ=255)                                                                                                                                     aaGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGccc                 >  7:330967/1‑149 (MQ=255)                                                                                                                                            gCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGa            <  2:33355/147‑1 (MQ=255)                                                                                                                                                ccaGGGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGGGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATGTAcc      <  4:186901/149‑1 (MQ=255)                                                                                                                                                    tGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATGTACCGCTa  >  2:357646/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CTGGACCTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATGTACCGCTA  >  NC_002947/2062703‑2062998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑