Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,116,508 A→G 8.7% N62D (AAT→GAT)  PP_1884 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,116,5080AG 8.7% 123.5 / 2.5 46N62D (AAT→GAT) PP_1884hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base G (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TGCAGTCTGACTACCGGATGTTGGGGTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAGCAGGTGGGGGCCCATTG  >  NC_002947/2116362‑2116656
                                                                                                                                                  |                                                                                                                                                    
tGCAGTCTGACTACCGGATGTTGGGGTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAAt                                                                                                                                                    >  1:144102/1‑149 (MQ=255)
   aGTCTGACTACCGGATGTTGGGGTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACATGGACCCTATCAGCGGTGCGACGGAGCCGGACAATGGt                                                                                                                                                 >  2:119636/1‑149 (MQ=255)
     tCTGACTACCGGATGTTGGGGTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTa                                                                                                                                               <  6:129084/149‑1 (MQ=255)
        gACTACCGGATGTTGGGGTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTAc                                                                                                                                              >  2:342727/1‑147 (MQ=255)
             ccGGATGTTGGGGTCGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGttt                                                                                                                                       <  8:74194/149‑1 (MQ=255)
             ccGGATGGTGGGGTGTAATACGATTTTCGCTATGCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGtt                                                                                                                                        <  8:252320/148‑1 (MQ=255)
                       gggTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGaa                                                                                                                             >  2:203598/1‑149 (MQ=255)
                                aCGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCt                                                                                                                    <  7:356314/149‑1 (MQ=255)
                                 cGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTg                                                                                                                   <  1:203598/149‑1 (MQ=255)
                                             tCCTGAAGGACAAGGCGGACGC‑TTATTGACCCAGGAATATATACTGCGCTTCAAGACCAGCGATTACCTGGACCCTATGAGCGGTGAGACGGCGCCGGTCAATGGTTACAAGGTTTATATTCAGAACTTTGTTCTGGATCACCCGCgg                                                                                                       <  2:217584/148‑1 (MQ=255)
                                                 gAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGt                                                                                                   >  3:300001/1‑149 (MQ=255)
                                                           gCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATaa                                                                                         >  4:185080/1‑149 (MQ=255)
                                                                   tttATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCt                                                                                                                    >  8:129414/1‑114 (MQ=255)
                                                                   tttATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCt                                                                                                                    <  7:129414/114‑1 (MQ=255)
                                                                   tttATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGata                                                                                 >  5:228435/1‑149 (MQ=255)
                                                                     tATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGata                                                                                 <  7:112461/147‑1 (MQ=255)
                                                                       ttGACCCATGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACTGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCAc                                                                             <  1:119636/149‑1 (MQ=255)
                                                                         gACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACGATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCg                                                                           >  4:113408/1‑149 (MQ=255)
                                                                         gACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACGATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCg                                                                           >  4:108112/1‑149 (MQ=255)
                                                                            ccAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGtt                                                                        >  6:204420/1‑149 (MQ=255)
                                                                              aGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGTCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTGATTCGAGAATGCCGATATCAGCGGTTcg                                                                      >  8:128118/1‑149 (MQ=255)
                                                                                gAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTcgcg                                                                    <  2:227137/149‑1 (MQ=255)
                                                                                 aaTATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGt                                                                   <  3:185080/149‑1 (MQ=255)
                                                                                    atataCGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGcc                                                                >  4:211481/1‑149 (MQ=255)
                                                                                     tataCGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACGATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCa                                                               <  3:108112/149‑1 (MQ=255)
                                                                                     tataCGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACGATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCa                                                               <  3:113408/149‑1 (MQ=255)
                                                                                        aCGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGcc                                                            >  1:106565/1‑149 (MQ=255)
                                                                                               ttCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCg                                                                                    <  1:240826/118‑1 (MQ=255)
                                                                                               ttCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCg                                                                                    >  2:240826/1‑118 (MQ=255)
                                                                                                                    gACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAAcc                                 <  1:342727/148‑1 (MQ=255)
                                                                                                                     aCCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGGTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAg                               >  2:159665/1‑149 (MQ=255)
                                                                                                                      cccTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAgg                              >  1:362036/1‑149 (MQ=255)
                                                                                                                       ccTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGa                                                   >  7:32727/1‑127 (MQ=255)
                                                                                                                       ccTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGa                                                   <  8:32727/127‑1 (MQ=255)
                                                                                                                       ccTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAgg                              >  6:209423/1‑148 (MQ=255)
                                                                                                                         tATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAg                           >  5:49594/1‑149 (MQ=255)
                                                                                                                           tCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAggg                         >  6:232140/1‑149 (MQ=255)
                                                                                                                            cAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAggg                         <  6:49594/148‑1 (MQ=255)
                                                                                                                             agcgccgagcCGGCGCCGGACAAGGGCTACCAGGCTTATATCCAGCACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATCCCGATACCCCCGGTTCGCGTGCCAGCCCGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCt                       <  4:255471/143‑1 (MQ=255)
                                                                                                                             aGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCt                       <  5:185536/149‑1 (MQ=255)
                                                                                                                                 gTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAg                   >  1:267187/1‑149 (MQ=255)
                                                                                                                                     gaCGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATAc                                             >  7:197434/1‑119 (MQ=255)
                                                                                                                                     gaCGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATAc                                             <  8:197434/119‑1 (MQ=255)
                                                                                                                                       cGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAGCAGGTg             <  3:211481/149‑1 (MQ=255)
                                                                                                                                            ccGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAGCAGGTGGGGGc        <  2:144102/149‑1 (MQ=255)
                                                                                                                                                 cAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAGCAGGTGGGGGCCCAtt   <  4:300001/149‑1 (MQ=255)
                                                                                                                                                  aaTGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAGCAGGTGGGGGCCCATTg  >  7:24506/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGCAGTCTGACTACCGGATGTTGGGGTGGAATACGATTTTCGCTATCCAGAAGGACAAGGCGGACGCTTTATTGACCCAGGAATATATACGGCGCTTCAAGACCAGCGCTTACCTGGACCCTATCAGCGGTGAGACGGCGCCGGACAATGGTTACAAGGTTTATATGCAGAACTTTGTTCTGGATCACCCGCGGTTGTCATTCGATAATGCCGATATCACCGGTTCGCGTGCCAGCCTGCGTATGAAAATACTCGGCGGCAACCAGGTAGGGCTGAAGCAGGTGGGGGCCCATTG  >  NC_002947/2116362‑2116656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑