Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,132,902 A→T 7.0% V37E (GTA→GAA)  PP_1890 ← type I pili chaperone protein FimC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,132,9020AT 7.0% 169.1 / 2.5 57V37E (GTA→GAA) PP_1890type I pili chaperone protein FimC
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base T (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TCGTATTGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGCAAGGACTGC  >  NC_002947/2132756‑2133047
                                                                                                                                                  |                                                                                                                                                  
tCGTATTGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTAcc                                                                                                                                                  >  4:50704/1‑149 (MQ=255)
  gTATTGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCg                                                                                                                                                 <  6:178913/148‑1 (MQ=255)
     ttGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCgaga                                                                                                                                              <  7:308496/148‑1 (MQ=255)
     ttGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCgaga                                                                                                                                              <  2:113669/148‑1 (MQ=255)
     ttGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCgaga                                                                                                                                              <  4:322875/148‑1 (MQ=255)
       gtcttcGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAgg                                                                                                                                            <  4:251814/148‑1 (MQ=255)
       gtcgtcGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGTGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAgg                                                                                                                                            <  1:338793/148‑1 (MQ=255)
        tcgtcgGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAggg                                                                                                                                           >  6:77005/1‑148 (MQ=255)
                          aTCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑Ca                                                                                                                        <  7:27691/148‑1 (MQ=255)
                           tCCACGCCTGCACGGCATAGATGCTTATCGATCGGTATCGAACAACGACGGATCTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAgg                                                                                                                      >  4:172750/1‑149 (MQ=255)
                             cACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAgg                                                                                                                      >  7:68690/1‑147 (MQ=255)
                                     cACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCaa                                                                                                             >  7:289291/1‑148 (MQ=255)
                                     cACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCaa                                                                                                             >  4:88086/1‑148 (MQ=255)
                                     cACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCaa                                                                                                             <  3:50704/148‑1 (MQ=255)
                                           aTAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGAGGAATGAATGTTTTCCAAGAGGGTAAGCATAAATAAGGA‑AAGGCAGAAACAaacaaca                                                                                                       >  8:233688/1‑147 (MQ=255)
                                           aTAGATGCTTTTCGATGGGTTTCGAACAACGACGGATATACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATTTCAAGGCGGAATGACTGTTTTCCGAGAGGGTAAGCAACAATAAGGT‑CAGGAAGCCGCAaacaaccc                                                                                                      >  8:236123/1‑147 (MQ=255)
                                              gATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑Ca                                                                                                                        >  5:263832/1‑128 (MQ=255)
                                              gATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑Ca                                                                                                                        <  6:263832/128‑1 (MQ=255)
                                                 gCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACTAcgcgtg                                                                                                >  7:259198/1‑147 (MQ=255)
                                                         aTGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTGTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGAAAACAACGACGCGGCCGGGGATc                                                                                        >  7:143997/1‑149 (MQ=255)
                                                              tttCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACgcgggcg                                                                                              <  5:36398/138‑1 (MQ=255)
                                                              tttCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACgcgggcg                                                                                              >  6:36398/1‑138 (MQ=255)
                                                              tttCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGAGCGGGTATCGTGaa                                                                                   >  6:95180/1‑149 (MQ=255)
                                                                  gAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGt                                                                                <  8:199877/148‑1 (MQ=255)
                                                                   aacaacGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGTCCAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGtt                                                                               <  1:72633/149‑1 (MQ=255)
                                                                         gacgGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCCTGAATGTTGACGGAt                                                                        >  3:324947/1‑149 (MQ=255)
                                                                         gaaggATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACACAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGCGCGCGTATCGTTAATGTTGACGGAt                                                                        >  7:80812/4‑149 (MQ=255)
                                                                            ggATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTgg                                                                     <  3:88086/149‑1 (MQ=255)
                                                                              aTGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGt                                                                    >  7:123112/1‑148 (MQ=255)
                                                                               tGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCa                                                                  <  5:77005/149‑1 (MQ=255)
                                                                                 tACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATc                                                                >  8:228292/1‑149 (MQ=255)
                                                                                  aCTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAg                                                                                                                             >  8:174724/1‑88 (MQ=255)
                                                                                  aCTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAg                                                                                                                             <  7:174724/88‑1 (MQ=255)
                                                                                   cTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCgg                                                              <  6:174465/149‑1 (MQ=255)
                                                                                   cTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCgg                                                              >  2:6868/1‑149 (MQ=255)
                                                                                      cGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCggg                                                             <  6:178730/147‑1 (MQ=255)
                                                                                       gTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTaa                                                          <  8:123112/149‑1 (MQ=255)
                                                                                          tGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACt                                                        <  4:324947/148‑1 (MQ=255)
                                                                                              gaggTTGAAAACGAGGCGCGTGTTGTTTAATGTCAATGCGGCATGACTTTTTTCCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCt                                                    <  7:233688/145‑1 (MQ=255)
                                                                                              gCCGTTGAAAACGTGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTTCCGAGAGGGTAAGCATAAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCt                                                    <  7:236123/148‑1 (MQ=255)
                                                                                              gCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAATAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCt                                                    <  2:160928/148‑1 (MQ=255)
                                                                                              gCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCt                                                    <  7:146331/148‑1 (MQ=255)
                                                                                                cGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCa                                                 <  3:172750/149‑1 (MQ=255)
                                                                                                      aaaCGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACgcgggcgg                                                                                             >  2:184814/1‑99 (MQ=255)
                                                                                                                    ttgttTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGAGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGcc                             <  5:95180/149‑1 (MQ=255)
                                                                                                                      gttTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTg                           >  2:131845/1‑149 (MQ=255)
                                                                                                                          aaTGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGCCGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGCCGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATg                       >  4:246291/1‑149 (MQ=255)
                                                                                                                              tCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGtt                    <  7:228292/148‑1 (MQ=255)
                                                                                                                                      gcATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCt                            >  1:336188/1‑132 (MQ=255)
                                                                                                                                      gcATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCt                            <  2:336188/132‑1 (MQ=255)
                                                                                                                                        aTGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGAAGCGGTCGGGTATCGTGAATGTTGACGGATTGGTAATCGGTTAACTCGCTCCAACCAGACGCAAAACAACGCCTGGATGGTTTAACCGTga           >  3:55590/1‑146 (MQ=255)
                                                                                                                                            cTGTTTACCTAGATGGTAAGCATCAATAACGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTGATCGTGTAACTCGCTGCATCCAGACGCACAACTACGCCTTGATTGTTTAACCGTGCAAGGa      <  4:55590/148‑1 (MQ=255)
                                                                                                                                            cTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGCAAGGAc     >  7:131458/1‑149 (MQ=255)
                                                                                                                                             tGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGc           >  2:258522/1‑142 (MQ=255)
                                                                                                                                             tGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGc           <  1:258522/142‑1 (MQ=255)
                                                                                                                                              gTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGCAAGGACTg   >  5:243905/1‑149 (MQ=255)
                                                                                                                                                ttACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGCAAGGACTGc  <  8:68690/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCGTATTGTCGTCGGCGTCCGTATTTATCCACGCCTGCACGGCATAGATGCTTTTCGATGGGTTTCGAACAACGACGGATGTACTGCGTTTGTCGCCGTTGAAAACGAGGCGCGTGTTGTTTAATGTCAAGGCGGCATGACTGTTTACCGAGAGGGTAAGCATCAATAAGGT‑CAGGCAGACGCAAACAACGACGCGGGCGGGTATCGTGAATGTTGACGGATTGGTCATCGGGTAACTCGCTGCATCCAGACGCACAACTACGCCTGGATGGTTTAACCGTGCAAGGACTGC  >  NC_002947/2132756‑2133047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑