Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,139,628 A→T 7.6% R165R (CGT→CGA)  PP_5481 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,139,6280AT 7.6% 189.3 / 5.1 66R165R (CGT→CGA) PP_5481hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base T (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

CTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAA  >  NC_002947/2139480‑2139771
                                                                                                                                                    |                                                                                                                                               
cTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcga                                                                                                                                                 <  5:145917/149‑1 (MQ=255)
cTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcga                                                                                                                                                 >  5:121393/1‑149 (MQ=255)
        ttCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCa                                                                                                                                         <  5:287741/149‑1 (MQ=255)
           tAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTg                                                                                                                                      >  6:364649/1‑149 (MQ=255)
             ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGc                                                                                                                                     <  5:364649/148‑1 (MQ=255)
             ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGcc                                                                                                                                    <  3:306622/149‑1 (MQ=255)
                 tgtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGCCggtg                                                                                                                                <  3:196733/149‑3 (MQ=255)
                   tCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTg                                                                                                                                      <  1:95869/141‑1 (MQ=255)
                   tCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTg                                                                                                                                      >  2:95869/1‑141 (MQ=255)
                     ggATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGCCggtgcaa                                                                                                                             <  6:142252/148‑6 (MQ=255)
                        tatCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCccacccac                                                                                                                         >  1:79178/1‑149 (MQ=255)
                          tCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACccacca                                                                                                                       <  1:212634/149‑1 (MQ=255)
                            aGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgc                                                                                                                     >  5:73443/1‑149 (MQ=255)
                             gCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcg                                                                                                                    >  2:351565/1‑149 (MQ=255)
                             gCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcg                                                                                                                    >  5:146025/1‑149 (MQ=255)
                              cATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcgc                                                                                                                   <  6:212242/149‑1 (MQ=255)
                                   tcACGGCGATCACCTTCATGCTGGTGGCCACTGCGTTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGAATTGGCGACGACTCCACTGCCGGCCCACCCACCAGCGCCAGTa                                                                                                              <  1:160/148‑1 (MQ=255)
                                   gCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTa                                                                                                              <  1:351565/149‑1 (MQ=255)
                                   gCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTa                                                                                                              <  1:6419/149‑1 (MQ=255)
                                     aCGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAg                                                                                                             >  7:163809/1‑148 (MQ=255)
                                         cGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGGCACTGCCGGCCCACCGACCAGCGCCAGTAGAGCAt                                                                                                        >  8:245350/1‑149 (MQ=255)
                                         cGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAt                                                                                                        >  8:245349/1‑149 (MQ=255)
                                          gATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtg                                                                                                       <  3:175308/149‑1 (MQ=255)
                                                cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCAc                                                                                                                                        <  3:56090/110‑1 (MQ=255)
                                                cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCAc                                                                                                                                        >  4:56090/1‑110 (MQ=255)
                                                 ttCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgag                                                                                                 >  4:204912/1‑148 (MQ=255)
                                                   cATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGaa                                                                                               <  2:79178/148‑1 (MQ=255)
                                                   cATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGaa                                                                                               <  3:204912/148‑1 (MQ=255)
                                                    aTGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGTCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGt                                                                                             <  3:113066/149‑1 (MQ=255)
                                                    aTGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAg                                                                                              >  5:199940/1‑148 (MQ=255)
                                                    aTGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGt                                                                                             <  6:73443/149‑1 (MQ=255)
                                                             gCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAgg                                                                                     <  5:139516/148‑1 (MQ=255)
                                                               cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAg                                                                                                                >  6:349285/1‑119 (MQ=255)
                                                               cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAg                                                                                                                <  5:349285/119‑1 (MQ=255)
                                                                    cGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAg                                                                             <  6:146025/149‑1 (MQ=255)
                                                                           ctACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcg                                                                      >  4:148014/1‑149 (MQ=255)
                                                                             aCGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgcc                                                                    >  5:12898/1‑149 (MQ=255)
                                                                              cGTTGTTTGCGTTAGCTTCCTGCTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATCGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgccg                                                                   >  2:163665/1‑149 (MQ=255)
                                                                               gttgttTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgccg                                                                   >  6:91903/1‑148 (MQ=255)
                                                                               gttgttTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACACACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgccg                                                                   >  5:271856/1‑148 (MQ=255)
                                                                                      gCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCAcaccac                                                           >  3:299307/1‑149 (MQ=255)
                                                                                       cGTTCGCTTGCTGGTCAGCGGCCGCTGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCAcaccac                                                           <  7:337305/148‑1 (MQ=255)
                                                                                        gTTCGCTTGCTGGTCAGCGGACGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATAGGAGACGACGCCACTGCCGGCCCACCCACCAGCGACAGTAGAACAGGGTCGAGAAGTAGGCAAGGATGCCCAGCCCCCCCCCGCCcaccc                                                            >  4:12199/1‑145 (MQ=255)
                                                                                         ttCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACaccacc                                                          >  1:356608/1‑147 (MQ=255)
                                                                                              ttGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccga                                                   <  7:245349/149‑1 (MQ=255)
                                                                                              ttGCTGGTCAGCGGCCGCGGCAAGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGACCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccga                                                   <  7:245350/149‑1 (MQ=255)
                                                                                                gCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccga                                                   <  6:121393/147‑1 (MQ=255)
                                                                                                        gCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGGAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAggg                                         >  6:196710/1‑149 (MQ=255)
                                                                                                             cgcgGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGcc                                    <  8:163809/149‑1 (MQ=255)
                                                                                                                      cGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTc                            <  5:91903/148‑1 (MQ=255)
                                                                                                                       gggCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGa                          <  6:215554/149‑1 (MQ=255)
                                                                                                                          ccTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAg                       >  4:132792/1‑149 (MQ=255)
                                                                                                                            tGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCt                     >  6:196935/1‑149 (MQ=255)
                                                                                                                                 ccTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCg                <  3:132792/149‑1 (MQ=255)
                                                                                                                                 ccTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCg                >  5:50823/1‑149 (MQ=255)
                                                                                                                                 ccTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCg                <  4:299307/149‑1 (MQ=255)
                                                                                                                                  cTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCgg               <  2:133580/149‑1 (MQ=255)
                                                                                                                                   ttGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGt              >  6:5855/1‑149 (MQ=255)
                                                                                                                                     gCACCGGCATTGGCGACGACGCCACTGCCGGCCCACACACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTg             <  6:271856/148‑1 (MQ=255)
                                                                                                                                      cACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGAt           >  4:151872/1‑149 (MQ=255)
                                                                                                                                       aCCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc          >  6:137497/1‑149 (MQ=255)
                                                                                                                                        ccGGCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc          >  6:229657/1‑148 (MQ=255)
                                                                                                                                         cGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCa        <  5:196935/149‑1 (MQ=255)
                                                                                                                                          ggCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCAc       <  6:199940/149‑1 (MQ=255)
                                                                                                                                          ggCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCAc       >  1:52312/1‑149 (MQ=255)
                                                                                                                                           gCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACt      >  3:17906/1‑149 (MQ=255)
                                                                                                                                                ggCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGaa  <  2:356608/148‑1 (MQ=255)
                                                                                                                                                 gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGa                                                                                                            >  2:360467/1‑41 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAA  >  NC_002947/2139480‑2139771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑