Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,147,209 T→C 5.0% intergenic (+82/+35) murB → / ← rne UDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,147,2090TC 5.0% 297.8 / 2.3 98intergenic (+82/+35)murB/rneUDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (49/44);  new base C (3/2);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGA  >  NC_002947/2147064‑2147355
                                                                                                                                                  |                                                                                                                                                  
gATTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCa                                                                                                                                                 <  7:101319/149‑1 (MQ=255)
 aTTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCAc                                                                                                                                                >  7:300412/1‑149 (MQ=255)
      ggCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCg                                                                                                                                           >  4:278446/1‑149 (MQ=255)
      ggCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCg                                                                                                                                           >  5:5795/1‑149 (MQ=255)
       gCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCgg                                                                                                                                          >  1:243031/1‑149 (MQ=255)
       gCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCgg                                                                                                                                          >  4:124837/1‑149 (MQ=255)
        cGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         <  4:247545/149‑1 (MQ=255)
        cGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCggg                                                                                                                                         <  6:213227/149‑1 (MQ=255)
        cGGATATCTTCGAGCGGGTTGGGCTTGAGCTGGAGCTGGAGCCAAATCTTTACTGATTTGCCGGCTGCGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         <  4:278525/149‑1 (MQ=39)
          gATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGc                                                                                                                                       >  1:252123/1‑149 (MQ=255)
                ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAAGGCCCCGAAGAAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACAAAAAAAACCCCCCCCAGTTTGAACTGGCGGGGCtttt                                                                                                                                   >  1:302704/1‑148 (MQ=255)
                ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCttttt                                                                                                                                  >  6:95298/1‑148 (MQ=255)
                  cGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCtttt                                                                                                                                   <  5:101045/145‑1 (MQ=255)
                          ttGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAACCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCttt                                                                                                                       >  5:320184/1‑149 (MQ=255)
                            ggggTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTgg                                                                                                                     >  8:18971/1‑149 (MQ=255)
                             gggTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGc                                                                                                                    >  7:316886/1‑149 (MQ=255)
                                  gAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATc                                                                                                                <  1:187782/148‑1 (MQ=255)
                                   aGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAg                                                                                                              <  8:316886/149‑1 (MQ=255)
                                      tGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGcc                                                                                                            >  2:112253/1‑148 (MQ=255)
                                        gagaTGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGt                                                                                                          <  5:95298/148‑1 (MQ=255)
                                        gagaTGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGt                                                                                                          >  3:170599/1‑148 (MQ=255)
                                        gagaTGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACC‑AAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGtg                                                                                                         <  4:341925/148‑1 (MQ=255)
                                            tGGAGCCTAATCTTTACTGATTTGCCGTCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGtgatga                                                                                                     <  3:230460/149‑1 (MQ=255)
                                            tGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGtgatga                                                                                                     <  2:252123/149‑1 (MQ=255)
                                             ggAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAg                                                                                                    >  5:327782/1‑149 (MQ=255)
                                             ggAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAg                                                                                                    >  8:117573/1‑149 (MQ=255)
                                              gAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAg                                                                                                    <  8:300412/148‑1 (MQ=255)
                                               aGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGt                                                                                                  >  4:189691/1‑149 (MQ=255)
                                                 ccTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                >  5:199156/1‑149 (MQ=255)
                                                 ccTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                <  3:124837/149‑1 (MQ=255)
                                                 ccTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                >  1:168853/1‑149 (MQ=255)
                                                 ccTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                <  6:176716/149‑1 (MQ=255)
                                                   tAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgt                                                                                              >  4:83455/1‑149 (MQ=255)
                                                   tAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgt                                                                                              >  8:43848/1‑149 (MQ=255)
                                                      tCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGTTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCt                                                                                           >  8:267553/1‑149 (MQ=255)
                                                         ttACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCAt                                                                                        >  2:327156/1‑149 (MQ=255)
                                                          tACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCAt                                                                                        <  6:327782/148‑1 (MQ=255)
                                                          tACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATc                                                                                       >  4:285162/1‑149 (MQ=255)
                                                               aTTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATcggcg                                                                                   <  7:89016/148‑1 (MQ=255)
                                                               aTTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATcggc                                                                                    <  4:170599/147‑1 (MQ=255)
                                                                    ccGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAACGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTcc                                                                             <  3:214577/149‑1 (MQ=255)
                                                                     cGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCa                                                                            <  1:327156/149‑1 (MQ=255)
                                                                      ggCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAg                                                                           >  5:192884/1‑149 (MQ=255)
                                                                       gCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAgg                                                                          <  3:269232/149‑1 (MQ=255)
                                                                           tgAA‑CCCCCGTAGGAGCTGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGc                                                                        <  5:207319/147‑1 (MQ=255)
                                                                           tgAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                       <  3:189691/148‑1 (MQ=255)
                                                                           tgAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                      <  2:168853/149‑1 (MQ=255)
                                                                             aa‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGGCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACGGGGGGGGGTGTTTGATTGAGCTTTGGGTATCAGCCGTGAGGGGGGTTGGGGTGATCGGGGGGTTCGCGGGCGGGt                                                                    >  2:319335/1‑149 (MQ=255)
                                                                             aa‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGt                                                                    <  7:43848/149‑1 (MQ=255)
                                                                                cccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTc                                                                  >  5:104995/1‑149 (MQ=255)
                                                                                  cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         >  4:108095/1‑76 (MQ=255)
                                                                                  cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         <  3:108095/76‑1 (MQ=255)
                                                                                  cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCt                                                                >  8:231438/1‑149 (MQ=255)
                                                                                  cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCt                                                                <  6:5795/149‑1 (MQ=255)
                                                                                   ccGTAGGAGCTGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTg                                                               >  8:218456/1‑149 (MQ=255)
                                                                                   ccGTAGGAGCGGGTTTACCCGCTAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTg                                                               <  3:62070/149‑1 (MQ=255)
                                                                                   ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCTGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTg                                                               <  3:278446/149‑1 (MQ=255)
                                                                                   ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTg                                                               >  2:166505/1‑149 (MQ=255)
                                                                                    cGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTgg                                                              <  7:290112/149‑1 (MQ=255)
                                                                                    cGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTgg                                                              >  5:252301/1‑149 (MQ=255)
                                                                                    cGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATACAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTgg                                                              >  3:354884/1‑149 (MQ=255)
                                                                                          aGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCt                                                        <  6:252301/149‑1 (MQ=255)
                                                                                               gTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagc                                                   <  8:294843/149‑1 (MQ=255)
                                                                                               gTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcag                                                    >  2:106995/1‑148 (MQ=255)
                                                                                                    cccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGTTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCt                                               <  7:267553/148‑1 (MQ=255)
                                                                                                    cccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTGCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCtt                                              >  7:15419/1‑149 (MQ=255)
                                                                                                    cccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCtt                                              >  3:305765/1‑149 (MQ=255)
                                                                                                    cccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCtt                                              >  2:1797/1‑149 (MQ=255)
                                                                                                       gcgAAGAGGCCGGTCAGGCAAACCAAAATAAGCCCCGCCAGGTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                           <  1:163753/149‑1 (MQ=255)
                                                                                                       gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                           <  3:285162/149‑1 (MQ=255)
                                                                                                       gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                           >  3:10633/1‑149 (MQ=255)
                                                                                                       gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                           >  6:57773/1‑149 (MQ=255)
                                                                                                       gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                           >  1:25235/1‑149 (MQ=255)
                                                                                                        cgAAGCGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCTGCAGCTTccca                                          <  7:218456/149‑3 (MQ=255)
                                                                                                               gCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATcggc                                                                                    >  8:284755/1‑100 (MQ=255)
                                                                                                                cccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCtt                                  <  1:112253/149‑1 (MQ=255)
                                                                                                                 ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagc                                                   <  5:369464/131‑1 (MQ=255)
                                                                                                                 ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagc                                                   >  6:369464/1‑131 (MQ=255)
                                                                                                                 ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCttt                                 >  2:102654/1‑149 (MQ=255)
                                                                                                                 ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCttt                                 <  6:320184/149‑1 (MQ=255)
                                                                                                                 ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCttt                                 <  3:224670/149‑1 (MQ=255)
                                                                                                                 ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCttt                                 <  6:199156/149‑1 (MQ=255)
                                                                                                                   gTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                               <  4:10633/149‑1 (MQ=255)
                                                                                                                       ggCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcag                            >  1:12981/1‑148 (MQ=255)
                                                                                                                        gCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcagca                          <  3:83455/149‑1 (MQ=255)
                                                                                                                           aaCCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGcc                       >  4:175240/1‑149 (MQ=255)
                                                                                                                             ccAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTc                     <  5:57773/149‑1 (MQ=255)
                                                                                                                              cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                    >  6:26042/1‑149 (MQ=255)
                                                                                                                              cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATACAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                    <  4:354884/149‑1 (MQ=255)
                                                                                                                               aaaaaaaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCgg                   >  7:188690/1‑149 (MQ=255)
                                                                                                                                       ccccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGc           <  6:104995/149‑1 (MQ=255)
                                                                                                                                        cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt          >  6:35425/1‑149 (MQ=255)
                                                                                                                                        cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                    <  2:40851/139‑1 (MQ=255)
                                                                                                                                        cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                    >  1:40851/1‑139 (MQ=255)
                                                                                                                                        cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt          >  8:308316/1‑149 (MQ=255)
                                                                                                                                        cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt          >  1:261896/1‑149 (MQ=255)
                                                                                                                                         ccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCtt         >  8:210963/1‑149 (MQ=255)
                                                                                                                                         ccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCtt         >  3:353471/1‑149 (MQ=255)
                                                                                                                                                tttGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGa  <  7:117573/149‑1 (MQ=255)
                                                                                                                                                tttGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGa  <  4:305765/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GATTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAA‑CCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGA  >  NC_002947/2147064‑2147355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑