Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 225,625 G→C 7.6% S12S (TCC→TCG)  PP_0173 ← transcriptional factor‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947225,6250GC 7.6% 228.6 / 6.4 76S12S (TCC→TCG) PP_0173transcriptional factor‑like protein
Reads supporting (aligned to +/- strand):  ref base G (33/36);  new base C (2/4);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CGTCACGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGCC  >  NC_002947/225477‑225761
                                                                                                                                                    |                                                                                                                                        
cGTCACGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAg                                                                                                                                          <  5:286272/149‑1 (MQ=255)
    aCGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACAGTCGGCTGAGCGTGGCGCGACTGGTGGCGAACGGCAAAAGATAACCTCCTTGTCTAGGCTTGCCGCAATACGAtc                                                                                                                                      >  6:227750/1‑148 (MQ=255)
     cGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATgg                                                                                                                                     <  4:47349/149‑1 (MQ=255)
           cgcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCaa                                                                                                                               <  1:167128/149‑2 (MQ=255)
            gcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATg                                                                                                                              >  4:360696/1‑149 (MQ=255)
            gcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATg                                                                                                                              >  3:290502/1‑149 (MQ=255)
            gcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATg                                                                                                                              >  6:327428/1‑149 (MQ=255)
             ccAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGc                                                                                                                             <  7:109047/149‑1 (MQ=255)
              cAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGcc                                                                                                                            <  4:229236/149‑2 (MQ=255)
                   cAGCTCGGCGCTGGTTATGCCTGGGGATCGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTggg                                                                                                                        <  5:227750/148‑1 (MQ=255)
                   cAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGcctagac                                                                                                                       <  8:152115/149‑7 (MQ=255)
                   cAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGcctaga                                                                                                                        >  7:152115/1‑143 (MQ=255)
                      cTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCAt                                                                                                                    <  7:332368/149‑1 (MQ=255)
                        cGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATgg                                                                                                                  >  3:266453/1‑149 (MQ=255)
                         ggCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATg                                                                                                                   <  7:313979/147‑1 (MQ=255)
                             cTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAAtt                                                                                                             <  1:26922/149‑1 (MQ=255)
                             cTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGGCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAAtt                                                                                                             <  8:200317/149‑1 (MQ=255)
                              tGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGAGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTg                                                                                                            >  2:379221/1‑149 (MQ=255)
                                gTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTgg                                                                                                           <  5:12068/148‑1 (MQ=255)
                                gTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGc                                                                                                          <  5:186512/149‑1 (MQ=255)
                                    tGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCcac                                                                                                       <  5:54128/148‑1 (MQ=255)
                                      ccTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGc                                                                                                    >  1:138655/1‑149 (MQ=255)
                                      ccTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGc                                                                                                    <  4:266453/149‑1 (MQ=255)
                                       cTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGcc                                                                                                   >  3:252427/1‑149 (MQ=255)
                                       cTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGcc                                                                                                   >  1:191959/1‑149 (MQ=255)
                                           ggAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCt                                                                                               <  1:89383/149‑1 (MQ=255)
                                             aaCGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCa                                                                                             <  6:264486/149‑1 (MQ=255)
                                                ggCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGa                                                                                           >  3:220080/1‑148 (MQ=255)
                                                ggCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGa                                                                                           >  2:316791/1‑148 (MQ=255)
                                                  cTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTc                                                                                              <  3:37189/143‑1 (MQ=255)
                                                  cTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTc                                                                                              >  4:37189/1‑143 (MQ=255)
                                                    gggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                      >  1:175142/1‑149 (MQ=255)
                                                    gggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGAGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGACACAGCCTGCTAAGATCAAg                                                                                      >  8:244473/1‑149 (MQ=255)
                                                     ggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                      <  4:290502/148‑1 (MQ=255)
                                                     ggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                      <  1:61753/148‑1 (MQ=255)
                                                      gCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCATGCCGCAATAGGATTGCGGCATGCGTGGGGCAAGGCAATTGGCCACAGCCTGCACAGATCAAgg                                                                                     >  7:260827/1‑148 (MQ=255)
                                                       cAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGaa                                                                                   <  4:252427/149‑1 (MQ=255)
                                                       cAAATGGAGCAGCAGCGTCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATATGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCATGGaa                                                                                   <  2:356975/149‑1 (MQ=255)
                                                         aCTGGCGCAGCAGCGCCCACTGTCTGCTGAGGGTGTCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGAATGGCATTTGGCCACAGCCTGCTCAGATCAAGGAAGt                                                                                 <  5:10102/149‑1 (MQ=255)
                                                          cTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTc                                                                                <  2:303138/149‑1 (MQ=255)
                                                                 agcagcGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTg                                                                         >  6:182309/1‑149 (MQ=255)
                                                                      cgcCCACTGTCGGCTGAGGGTGGCGCGAGAGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGc                                                                    <  1:379221/149‑1 (MQ=255)
                                                                         ccACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTc                                                                 >  3:82459/1‑149 (MQ=255)
                                                                          cACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCa                                                                                        <  8:96861/125‑1 (MQ=255)
                                                                          cACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCa                                                                                        >  7:96861/1‑125 (MQ=255)
                                                                                 ggCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCgg                                                          <  3:360696/148‑1 (MQ=255)
                                                                                 ggCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgt                                                         <  4:82459/149‑1 (MQ=255)
                                                                                  gCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgtt                                                        >  3:202984/1‑149 (MQ=255)
                                                                                   cTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttc                                                       <  5:327428/149‑1 (MQ=255)
                                                                                   cTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttc                                                       <  7:186391/149‑1 (MQ=255)
                                                                                    tGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttcg                                                      >  8:273689/1‑149 (MQ=255)
                                                                                       gggTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                      >  4:12191/1‑114 (MQ=255)
                                                                                       gggTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttcgttc                                                   <  8:106293/149‑1 (MQ=255)
                                                                                        ggTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttcgttc                                                   <  2:138655/148‑1 (MQ=255)
                                                                                         gTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTcgc                                                 >  8:240583/1‑149 (MQ=255)
                                                                                          tGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTcgc                                                 >  4:237793/1‑148 (MQ=255)
                                                                                             cgcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGa                                               >  8:26706/1‑147 (MQ=255)
                                                                                             cgcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACa                                             <  6:321792/149‑1 (MQ=255)
                                                                                              gcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGAt                                                                                          <  1:285601/103‑1 (MQ=255)
                                                                                              gcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGAt                                                                                          >  2:285601/1‑103 (MQ=255)
                                                                                                  gtggtgGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGttt                                        <  7:93546/149‑1 (MQ=255)
                                                                                                   tggttgCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGAATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCTACAGGttt                                        <  7:244473/148‑1 (MQ=255)
                                                                                                          gAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGa                                >  2:247608/1‑149 (MQ=255)
                                                                                                           aaCGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGAc                               <  7:273689/149‑1 (MQ=255)
                                                                                                            aCGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACa                              >  1:340018/1‑149 (MQ=255)
                                                                                                                     gATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAg                      >  2:253138/1‑148 (MQ=255)
                                                                                                                      aTAACGTCCTAATCTAGGCTTCCCGCAATAGGATGGCGGCATGCGTCGGGAATGGCAATTGGCCCCAGCCTGCTCAGATCATGTAAGTCGCATTTGGTTGCCTCGTGCCGGTACGTTCGCGAAAGCTTTTGACGAGGCATCACGCAGaa                    >  4:279202/1‑149 (MQ=255)
                                                                                                                         aCGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATc                  <  4:220080/148‑1 (MQ=255)
                                                                                                                          cGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTTCTGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCAc                <  1:316791/149‑1 (MQ=255)
                                                                                                                           gTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACg               >  6:293713/1‑149 (MQ=255)
                                                                                                                                 gTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcat         >  2:2227/1‑149 (MQ=255)
                                                                                                                                   cTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcatc        <  4:202984/148‑1 (MQ=255)
                                                                                                                                    tAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcatca       >  5:93780/1‑148 (MQ=255)
                                                                                                                                      ggCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcatca       <  3:92130/146‑1 (MQ=255)
                                                                                                                                       gCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGc                                                                    >  8:17408/1‑84 (MQ=255)
                                                                                                                                       gCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGc                                                                    <  7:17408/84‑1 (MQ=255)
                                                                                                                                         ttGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGcc  >  4:122008/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
CGTCACGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGCC  >  NC_002947/225477‑225761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑