Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,185,785	C→G	5.6%	R608R (CGG→CGC)	PP_1936 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,185,785	0	C	G	5.6%	213.0 / 3.1	71	R608R (CGG→CGC)	PP_1936	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (37/30);  new base G (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

TGTGTCGCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCAGTTTTAGGTAA  >  NC_002947/2185643‑2185932                                                                                                                                               |                                                                                                                                                     tgtgTCGCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGAGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCa                                                                                                                                                >  7:123795/1‑149 (MQ=255)   tgtCGCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATg                                                                                                                                              <  1:111831/149‑1 (MQ=255)     tCGCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCt                                                                                                                                            >  5:73525/1‑149 (MQ=255)     tCGCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAACGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCGGGCTCATgat                                                                                                                                            <  5:19356/149‑3 (MQ=255)       gCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTg                                                                                                                                          <  2:62014/149‑1 (MQ=255)        cAGGCTGCCATGGAATCGTCCGATAGTTGCATATCGGTGGAGGTATTCAATGAAGGTGTTGATAGCATCTTCAGGGTCAATATTGTTGATGCTGGAAGTGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCttta                                                                                                                                         <  8:123795/149‑3 (MQ=255)        cAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGATGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGa                                                                                                                                         <  7:286324/149‑1 (MQ=255)        cAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGa                                                                                                                                         >  5:272106/1‑149 (MQ=255)         aGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGAt                                                                                                                                        <  3:61362/149‑1 (MQ=255)         aGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGAt                                                                                                                                        <  1:28679/149‑1 (MQ=255)         aGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGAt                                                                                                                                        <  7:72386/149‑1 (MQ=255)                  tCGATTCGTCAGATAGATGCATATCGGGGGATTTATTGAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGaa                                                                                                                               <  6:272106/149‑1 (MQ=255)                  tCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTTATGCTTGATAAGATTGaa                                                                                                                               <  5:175163/149‑1 (MQ=255)                  tCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTCGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGaa                                                                                                                               <  4:305316/149‑1 (MQ=255)                            cGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑tttatctc                                                                                                                     >  1:325190/1‑143 (MQ=255)                                aGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGtt                                                                                                                 >  1:247038/1‑148 (MQ=255)                                    gCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAAt                                                                                                            >  5:140367/1‑149 (MQ=255)                                            ggggAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGa                                                                                                    >  2:237607/1‑149 (MQ=255)                                             gggATGTTTTAAATCAATGTGTTTATATCATATTTAGCGGCAATATTGTTGAAGCTGGAATATCTTTTGACGATCATCCTCCAAAATTGATCATGATCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGc                                                                                                      <  8:232783/146‑1 (MQ=255)                                                 ggTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TGTTTTGATGTTAAAATGTTAGCGAttttt                                                                                               >  8:106899/1‑149 (MQ=255)                                                   tATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGAtttttt                                                                                              >  7:143697/1‑148 (MQ=255)                                                     ttCAATCAAGGTGTTGATAGCATCTTAAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGATTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCAc                                                                                           >  5:363276/1‑149 (MQ=255)                                                        aatcaaGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCAt                                                                                        >  1:305345/1‑149 (MQ=255)                                                        aatcaaGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGATGGAAGATCTGTTGCCGAGCATCCTCCAAAATTGATAATGAGCCCGCTCATGATTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACAAt                                                                                        >  5:80509/1‑149 (MQ=255)                                                          tcaaGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCAtt                                                                                       >  2:61161/1‑148 (MQ=255)                                                           caaGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATtca                                                                                     >  3:150955/1‑149 (MQ=255)                                                              ggTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATtcata                                                                                   >  5:330020/1‑147 (MQ=255)                                                               gtgtTGATAGCATCATCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGAAGTTAAAATGGTAGCGATTTTTTCAACATTCAacac                                                                                 >  8:167054/1‑149 (MQ=255)                                                                 gatGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACt                                                                                <  1:81008/146‑1 (MQ=255)                                                                  ttGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGa                                                                              <  2:219500/149‑1 (MQ=255)                                                                  ttGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGa                                                                              <  6:140367/149‑1 (MQ=255)                                                                      tAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAACAGCTGTAGAAGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGtt                                                                           >  5:82818/1‑148 (MQ=255)                                                                                 gCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTg                                                               <  7:167054/149‑1 (MQ=255)                                                                                           ggTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAgcgc                                                      >  4:42760/1‑148 (MQ=255)                                                                                               gAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGc                                                                     >  6:184189/1‑129 (MQ=255)                                                                                               gAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGc                                                                     <  5:184189/129‑1 (MQ=255)                                                                                               gAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGCAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAg                                                  <  6:73525/148‑1 (MQ=255)                                                                                                 aGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTaa                                                                         <  2:356424/123‑1 (MQ=255)                                                                                                   cTGGAAGAGCTGTTGACGACCAGCCTACAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGTTATc                                             <  1:271463/149‑1 (MQ=255)                                                                                                    tGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATcc                                            >  2:195793/1‑149 (MQ=255)                                                                                                     ggAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCt                                           >  4:353692/1‑149 (MQ=255)                                                                                                      gAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCt                                           <  1:237607/148‑1 (MQ=255)                                                                                                       aaGATCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGa                                         <  5:366089/149‑1 (MQ=255)                                                                                                        agagCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAg                                        <  2:298214/149‑1 (MQ=255)                                                                                                              gTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGa                                         >  6:75656/1‑142 (MQ=255)                                                                                                              gTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGa                                         <  5:75656/142‑1 (MQ=255)                                                                                                              gTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCg                                  >  8:294515/1‑149 (MQ=255)                                                                                                              gTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCg                                  >  8:143270/1‑149 (MQ=255)                                                                                                                 gACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGATTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCgggg                               >  5:101795/1‑149 (MQ=255)                                                                                                                  aCGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGt                              <  8:143697/149‑1 (MQ=255)                                                                                                                    gAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTTGCGATTTTTTCACCATTGATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGATGCTCCGGGTTgg                            >  8:197477/1‑149 (MQ=255)                                                                                                                    gAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTgg                            >  5:230841/1‑149 (MQ=255)                                                                                                                    gAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTgg                            >  1:290355/1‑149 (MQ=255)                                                                                                                    gAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTgg                            >  3:130906/1‑149 (MQ=255)                                                                                                                       cagcCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCTCGAAGGTATCCTGAGGCTCCGGGGTGGCa                          >  4:349059/1‑148 (MQ=255)                                                                                                                         gcCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAAt                       <  2:247038/149‑1 (MQ=255)                                                                                                                            tCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTATTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCt                     >  7:352277/1‑148 (MQ=255)                                                                                                                              cAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTaa                   >  1:5129/1‑148 (MQ=255)                                                                                                                              cAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTaa                   <  6:363276/148‑1 (MQ=255)                                                                                                                               aaaaTTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAAc                  >  1:364389/1‑148 (MQ=255)                                                                                                                                  aTTGATCATGATCCCGCTCATGCTTGATAAGATTTAAG‑TTTTTTGATGTTAAAATGGTAGAGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATc              <  5:58589/149‑1 (MQ=255)                                                                                                                                   ttGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCa             <  2:305345/149‑1 (MQ=255)                                                                                                                                     aagcATGAGCGGGCTCATGCTTGATAAGATTGAAGTTTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTaa                   >  1:66917/4‑142 (MQ=255)                                                                                                                                     aagcATGAGCGGGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTaa                   <  2:66917/138‑1 (MQ=255)                                                                                                                                     aagcATGAGCGGGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCAGt           >  4:142639/4‑149 (MQ=255)                                                                                                                                      aTCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTACACCATTCATCACTGAGATAAAAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGATACGGGGTGGCAAATCTAACAATCAGtt          >  7:104029/1‑149 (MQ=255)                                                                                                                                       tCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCAGttt         <  2:5129/149‑1 (MQ=255)                                                                                                                                       tCATGAGCCCGCTCATGCTTGATAAGATTCAAG‑TTTTTTCATGTTAAAATGGTATCGATTTTTTCACCATTCATCACTGAGTTAACATCTTCTAGGAACACCGCGAAGGTATCCTGAGTCTACGGGGTGGCAAATCTAACAATAAGatt         >  2:351491/1‑149 (MQ=255)                                                                                                                                         aTGAGCCCGCTCATGCATGATAAGATTGAAC‑TTTTTTGATGTTAAAATGGTAGCGAATTTTTCACAATTCATCACTCAGTTAACAGCGTCATGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACCATcctgtt         >  4:69536/1‑142 (MQ=255)                                                                                                                                             gCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCAGTTTTAGGt    <  2:364389/148‑1 (MQ=255)                                                                                                                                             gCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTAATCACTGAGTTAACAGCTTCTTGGTAATGCGCGAAGGTTTCATGTTGATCCGTGTTGGAAAATCTAACATTAAGTTTTAGGt    >  3:229709/1‑148 (MQ=255)                                                                                                                                              cccGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCAGTTTTAGGTaa  <  1:61161/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     TGTGTCGCAGGCTGCCATCGAATCGTCCGATAGATGCATATCGGGGGAGGTATTCAATCAAGGTGTTGATAGCATCTTCAGCGGCAATATGGTTGAAGCTGGAAGAGCTGTTGACGAGCAGCCTCCAAAATTGATCATGAGCCCGCTCATGCTTGATAAGATTGAAG‑TTTTTTGATGTTAAAATGGTAGCGATTTTTTCACCATTCATCACTGAGTTAACAGCTTCTTGGTACAGCGCGAAGGTATCCTGAGGCTCCGGGGTGGCAAATCTAACAATCAGTTTTAGGTAA  >  NC_002947/2185643‑2185932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑