Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,191,853 A→G 11.7% Q907R (CAG→CGG)  PP_1937 → helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,191,8530AG11.7% 126.4 / 8.1 51Q907R (CAG→CGG) PP_1937helicase
Reads supporting (aligned to +/- strand):  ref base A (25/20);  new base G (3/3);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

ATGAGCTTTATCGAAACGAAACTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGCTACTGC  >  NC_002947/2191712‑2191999
                                                                                                                                             |                                                                                                                                                  
aTGAGCTTTATCGAAACGAAACTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCGGCTGGt                                                                                                                                              >  8:27012/1‑148 (MQ=255)
aTGAGCTTTATCGAAACGAAACTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCGGCTGGt                                                                                                                                              <  7:27012/148‑1 (MQ=255)
 tGAGCTTTATCGAAACGAAACTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGGCCAGCCGGtca                                                                                                                                            <  4:14259/149‑1 (MQ=255)
         aTCGAAACGAAACTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACAAGCCGGTCATCAAtctc                                                                                                                                    >  5:116174/1‑149 (MQ=255)
           cgaaacgaaaCTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCGGCTGGtcatcag                                                                                                                                        >  8:28832/1‑142 (MQ=255)
           cgaaacgaaaCTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCGGCTGGtcatcag                                                                                                                                        <  7:28832/143‑2 (MQ=255)
              aacgaaacTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCcgc                                                                                                                                 >  8:184211/1‑147 (MQ=255)
                 aaaacTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGAt                                                                                                                            <  6:110296/148‑1 (MQ=255)
                  aaacTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTCATGACCAGCAGGTCATCAATCTCCGCGAGAtt                                                                                                                           >  6:263031/1‑149 (MQ=255)
                       aaCAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAAc                                                                                                                      >  5:106153/1‑149 (MQ=255)
                            ggTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGc                                                                                                                 >  8:349852/1‑149 (MQ=255)
                                 tGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAgg                                                                                                            <  7:219775/149‑1 (MQ=255)
                                   tCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTGTGCTTGGACGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCATGTCCGGATCTGATGAGAAGCCGGTCTTCAATCTCCGCTTGATTTCATCGTAGATGCggt                                                                                                           >  8:347058/1‑147 (MQ=255)
                                               gTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGa                                                                                              <  5:129326/149‑1 (MQ=255)
                                                   tCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGAt                                                                                          >  3:153467/1‑149 (MQ=255)
                                                      cTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTg                                                                                       <  7:184897/149‑1 (MQ=255)
                                                      cTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTg                                                                                       <  6:40001/149‑1 (MQ=255)
                                                         ccAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGc                                                                                      <  7:99627/147‑1 (MQ=255)
                                                                     gggTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAAcc                                                                          <  6:116174/147‑1 (MQ=255)
                                                                       gtgtTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGa                                                                       >  6:311777/1‑148 (MQ=255)
                                                                       gtgtTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGa                                                                       <  5:311777/148‑1 (MQ=255)
                                                                            tctcTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAggg                                                                 >  5:47295/1‑149 (MQ=255)
                                                                              tctGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCt                                                               >  7:150981/1‑149 (MQ=255)
                                                                              tctGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCt                                                               >  4:246558/1‑149 (MQ=255)
                                                                              tctGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGACCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCt                                                               >  5:105115/1‑149 (MQ=255)
                                                                                tGCGTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGc                                                                <  2:101370/146‑1 (MQ=255)
                                                                                      gCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATgg                                                        <  6:106153/148‑1 (MQ=255)
                                                                                        cGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCa                                                     >  3:342063/1‑149 (MQ=255)
                                                                                           tGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGAtt                                                                                         <  4:106511/110‑1 (MQ=255)
                                                                                           tGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGAtt                                                                                         >  3:106511/1‑110 (MQ=255)
                                                                                              gaATGCGCGCGCACCAAGGTGGATTTTCCTGTCCTGCTCTGATGACCATCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATATGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCAtgctgc                                               <  5:263031/149‑1 (MQ=255)
                                                                                                                gTGGATTTTCCTGTCCAGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGc                             >  4:219546/1‑149 (MQ=255)
                                                                                                                   gATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTgg                          <  7:184211/149‑1 (MQ=255)
                                                                                                                     ttttCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCGAGGCTGGGc                        >  3:13051/1‑149 (MQ=255)
                                                                                                                           tGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAAc                                                                                                                      >  7:61748/1‑49 (MQ=255)
                                                                                                                           tGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAAc                                                                                                                      <  8:61748/49‑1 (MQ=255)
                                                                                                                           tGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATg                  >  4:334579/1‑149 (MQ=255)
                                                                                                                                ggCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGgaga              >  2:20860/1‑148 (MQ=255)
                                                                                                                                 gCTCTGCTGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGaaa            <  4:153467/149‑1 (MQ=255)
                                                                                                                                 gCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGaa             >  1:318234/1‑148 (MQ=255)
                                                                                                                                  ctctGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCa                                              >  7:63019/1‑114 (MQ=255)
                                                                                                                                  ctctGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCa                                              <  8:63019/114‑1 (MQ=255)
                                                                                                                                   attgatgaCCGGCTTTTCATCAATCTCAGCTTGATTGCAAGGCATCTGAGGGCACGGTTATCAGACTATTTGCAGAAGCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTTGGCGAGATGGAGaaaa           <  2:226222/146‑1 (MQ=255)
                                                                                                                                   attgatgaCCGGCTGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTTCAGAACCTCAACCCGACAAGTGCTGCAATGGGCAAGCTGCAATCTGTTCTGGCCAGGCAGGGCGAGATGGAGaaaa           >  1:226222/3‑148 (MQ=255)
                                                                                                                                     tgatgaCCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGc        >  8:179699/1‑149 (MQ=255)
                                                                                                                                     tgatgaCCAGCCGGTCATCAATCTCCGCGAGATTCCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGc        >  2:68493/1‑149 (MQ=255)
                                                                                                                                       atgaCCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATg         <  3:219546/146‑1 (MQ=255)
                                                                                                                                       atgaCCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGc        <  6:47295/147‑1 (MQ=255)
                                                                                                                                         gaCCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGCTACt    >  8:193714/1‑149 (MQ=255)
                                                                                                                                           ccAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGCTACTGc  <  8:150981/149‑1 (MQ=255)
                                                                                                                                           ccAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGCTACTGc  >  4:371212/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
ATGAGCTTTATCGAAACGAAACTAACAAGGTCATGTCGCGTGTGAGCGTTGTCCCTTCCAGTGAACCTTGGGTGTTTCTCTGCTTGGCCGGTGAGAATGCGCGCGCACCAAGGTGGATTTTCCTGTCCGGCTCTGATGACCAGCCGGTCATCAATCTCCGCGAGATTGCAACGCAGCTGAGGGCACGGTTATCAGACGATTTGCAGAACCTCAACCCGACAAGGGCTGCAATGGGCATGCTGCAATCGTTTCTGGCCAGGCTGGGCGAGATGGAGAAAATGCTACTGC  >  NC_002947/2191712‑2191999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑