Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 225,629 G→T 7.8% P11Q (CCA→CAA)  PP_0173 ← transcriptional factor‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947225,6290GT 7.8% 224.7 / 6.0 75P11Q (CCA→CAA) PP_0173transcriptional factor‑like protein
Reads supporting (aligned to +/- strand):  ref base G (32/37);  new base T (2/4);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGCCGAAGGTTGGGGTTGT  >  NC_002947/225482‑225776
                                                                                                                                                   |                                                                                                                                                   
cGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATgg                                                                                                                                                    <  4:47349/149‑1 (MQ=255)
      cgcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCaa                                                                                                                                              <  1:167128/149‑2 (MQ=255)
       gcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATg                                                                                                                                             >  4:360696/1‑149 (MQ=255)
       gcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATg                                                                                                                                             >  3:290502/1‑149 (MQ=255)
       gcCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATg                                                                                                                                             >  6:327428/1‑149 (MQ=255)
        ccAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGc                                                                                                                                            <  7:109047/149‑1 (MQ=255)
         cAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGcc                                                                                                                                           <  4:229236/149‑2 (MQ=255)
              cAGCTCGGCGCTGGTTATGCCTGGGGATCGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTggg                                                                                                                                       <  5:227750/148‑1 (MQ=255)
              cAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGcctagac                                                                                                                                      <  8:152115/149‑7 (MQ=255)
              cAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCCATCCTATTGCGGCAAGcctaga                                                                                                                                       >  7:152115/1‑143 (MQ=255)
                 cTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCAt                                                                                                                                   <  7:332368/149‑1 (MQ=255)
                   cGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATgg                                                                                                                                 >  3:266453/1‑149 (MQ=255)
                    ggCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATg                                                                                                                                  <  7:313979/147‑1 (MQ=255)
                        cTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAAtt                                                                                                                            <  1:26922/149‑1 (MQ=255)
                        cTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGGCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAAtt                                                                                                                            <  8:200317/149‑1 (MQ=255)
                         tGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGAGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTg                                                                                                                           >  2:379221/1‑149 (MQ=255)
                           gTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTgg                                                                                                                          <  5:12068/148‑1 (MQ=255)
                           gTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGc                                                                                                                         <  5:186512/149‑1 (MQ=255)
                               tGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCcac                                                                                                                      <  5:54128/148‑1 (MQ=255)
                                 ccTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGc                                                                                                                   >  1:138655/1‑149 (MQ=255)
                                 ccTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGc                                                                                                                   <  4:266453/149‑1 (MQ=255)
                                  cTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGcc                                                                                                                  >  3:252427/1‑149 (MQ=255)
                                  cTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGcc                                                                                                                  >  1:191959/1‑149 (MQ=255)
                                      ggAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCt                                                                                                              <  1:89383/149‑1 (MQ=255)
                                        aaCGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCa                                                                                                            <  6:264486/149‑1 (MQ=255)
                                           ggCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGa                                                                                                          >  3:220080/1‑148 (MQ=255)
                                           ggCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGa                                                                                                          >  2:316791/1‑148 (MQ=255)
                                             cTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTc                                                                                                             >  4:37189/1‑143 (MQ=255)
                                             cTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTc                                                                                                             <  3:37189/143‑1 (MQ=255)
                                               gggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                                     >  1:175142/1‑149 (MQ=255)
                                               gggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGAGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGACACAGCCTGCTAAGATCAAg                                                                                                     >  8:244473/1‑149 (MQ=255)
                                                ggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                                     <  4:290502/148‑1 (MQ=255)
                                                ggCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                                     <  1:61753/148‑1 (MQ=255)
                                                 gCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCATGCCGCAATAGGATTGCGGCATGCGTGGGGCAAGGCAATTGGCCACAGCCTGCACAGATCAAgg                                                                                                    >  7:260827/1‑148 (MQ=255)
                                                  cAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGaa                                                                                                  <  4:252427/149‑1 (MQ=255)
                                                  cAAATGGAGCAGCAGCGTCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATATGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCATGGaa                                                                                                  <  2:356975/149‑1 (MQ=255)
                                                    aCTGGCGCAGCAGCGCCCACTGTCTGCTGAGGGTGTCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGAATGGCATTTGGCCACAGCCTGCTCAGATCAAGGAAGt                                                                                                <  5:10102/149‑1 (MQ=255)
                                                     cTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTc                                                                                               <  2:303138/149‑1 (MQ=255)
                                                            agcagcGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTg                                                                                        >  6:182309/1‑149 (MQ=255)
                                                                 cgcCCACTGTCGGCTGAGGGTGGCGCGAGAGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGc                                                                                   <  1:379221/149‑1 (MQ=255)
                                                                    ccACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTc                                                                                >  3:82459/1‑149 (MQ=255)
                                                                     cACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCa                                                                                                       >  7:96861/1‑125 (MQ=255)
                                                                     cACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCa                                                                                                       <  8:96861/125‑1 (MQ=255)
                                                                            ggCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCgg                                                                         <  3:360696/148‑1 (MQ=255)
                                                                            ggCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgt                                                                        <  4:82459/149‑1 (MQ=255)
                                                                             gCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgtt                                                                       >  3:202984/1‑149 (MQ=255)
                                                                              cTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttc                                                                      <  5:327428/149‑1 (MQ=255)
                                                                              cTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttc                                                                      <  7:186391/149‑1 (MQ=255)
                                                                               tGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttcg                                                                     >  8:273689/1‑149 (MQ=255)
                                                                                  gggTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAg                                                                                                     >  4:12191/1‑114 (MQ=255)
                                                                                  gggTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttcgttc                                                                  <  8:106293/149‑1 (MQ=255)
                                                                                   ggTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGgttcgttc                                                                  <  2:138655/148‑1 (MQ=255)
                                                                                    gTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTcgc                                                                >  8:240583/1‑149 (MQ=255)
                                                                                     tGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTcgc                                                                >  4:237793/1‑148 (MQ=255)
                                                                                        cgcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGa                                                              >  8:26706/1‑147 (MQ=255)
                                                                                        cgcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACa                                                            <  6:321792/149‑1 (MQ=255)
                                                                                         gcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGAt                                                                                                         <  1:285601/103‑1 (MQ=255)
                                                                                         gcgAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGAt                                                                                                         >  2:285601/1‑103 (MQ=255)
                                                                                             gtggtgGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGttt                                                       <  7:93546/149‑1 (MQ=255)
                                                                                              tggttgCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGAATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCTACAGGttt                                                       <  7:244473/148‑1 (MQ=255)
                                                                                                     gAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGa                                               >  2:247608/1‑149 (MQ=255)
                                                                                                      aaCGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGAc                                              <  7:273689/149‑1 (MQ=255)
                                                                                                       aCGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACa                                             >  1:340018/1‑149 (MQ=255)
                                                                                                                gATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAg                                     >  2:253138/1‑148 (MQ=255)
                                                                                                                 aTAACGTCCTAATCTAGGCTTCCCGCAATAGGATGGCGGCATGCGTCGGGAATGGCAATTGGCCCCAGCCTGCTCAGATCATGTAAGTCGCATTTGGTTGCCTCGTGCCGGTACGTTCGCGAAAGCTTTTGACGAGGCATCACGCAGaa                                   >  4:279202/1‑149 (MQ=255)
                                                                                                                    aCGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATc                                 <  4:220080/148‑1 (MQ=255)
                                                                                                                     cGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTTCTGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCAc                               <  1:316791/149‑1 (MQ=255)
                                                                                                                      gTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACg                              >  6:293713/1‑149 (MQ=255)
                                                                                                                            gTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcat                        >  2:2227/1‑149 (MQ=255)
                                                                                                                              cTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcatc                       <  4:202984/148‑1 (MQ=255)
                                                                                                                               tAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcatca                      >  5:93780/1‑148 (MQ=255)
                                                                                                                                 ggCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCtcatca                      <  3:92130/146‑1 (MQ=255)
                                                                                                                                  gCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGc                                                                                   >  8:17408/1‑84 (MQ=255)
                                                                                                                                  gCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGc                                                                                   <  7:17408/84‑1 (MQ=255)
                                                                                                                                    ttGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGcc                 >  4:122008/1‑148 (MQ=255)
                                                                                                                                                  tGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGCCGAAGGTTGGGGTtgt  <  3:237793/149‑1 (MQ=255)
                                                                                                                                                   ggcggcATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGCCGAAGGTTGGGGTtgt  <  6:93780/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCAGGCGCCAGACCAGCTCGGCGCTGGTTATGCCTGGGGAACGGCTGGGCAACTGGCGCAGCAGCGCCCACTGTCGGCTGAGGGTGGCGCGAGTGGTGGCGAACGGCAAAAGATAACGTCCTTGTCTAGGCTTGCCGCAATAGGATGGCGGCATGCGTGGGGCATGGCAATTGGCCACAGCCTGCTCAGATCAAGGAAGTCGCATTTGGTTGCCTCGTGCCGGTTCGTTCGCGACAGGTTTTGACGTGACATCACGCAGAATCACGCCTCATCACAGCCGAAGGTTGGGGTTGT  >  NC_002947/225482‑225776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑