| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,223,369 | G→C | 10.4% | P393R (CCG→CGG) | PP_1962 ← | site‑specific recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,223,369 | 0 | G | C | 10.4% | 162.6 / 6.0 | 57 | P393R (CCG→CGG) | PP_1962 | site‑specific recombinase |
| Reads supporting (aligned to +/- strand): ref base G (25/26); new base C (3/3); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
ATAGGAATGGTGGCCGAACAGCAGACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTAGAGG > NC_002947/2223221‑2223505 | aTAGGAATGGTGGCCGAACAGCAGACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCg > 6:291623/1‑149 (MQ=255) agcagACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCCGCAGCTGCCGAacacaatc < 1:242643/149‑5 (MQ=255) agACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTg < 5:81259/147‑1 (MQ=255) agACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTg > 6:81259/1‑147 (MQ=255) agACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCCGCAGCTGCCGAacacaatcatt < 6:180442/149‑8 (MQ=255) agACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCCGCAGCTGCCGAacacaatca < 1:115936/147‑6 (MQ=255) agACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCCGCAGCTGCCGAacacaatca > 2:115936/1‑142 (MQ=255) agACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGAGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCCGCAGCTGCCGAacacaatcatt > 5:180442/1‑142 (MQ=255) gACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGt > 2:193761/1‑148 (MQ=255) aCGGATCAGTTCAATATTTCTTTCGCTCCGATTGATCAGGATTTCTTCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTa < 2:340669/149‑1 (MQ=255) gATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCt < 3:173765/149‑1 (MQ=255) gTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCTATGCGGATGGAGGCGCTCGATCACCGTCAC‑‑TTCTTAGAGATAATCAATGA‑TTGTGTTCGGAAGCTGCGGAAAACCTTTGGTTAACTTGATc > 5:317414/1‑148 (MQ=255) gTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGAAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATc > 6:380650/1‑148 (MQ=255) tCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACATTTGGTTACCTTGATCga > 4:360974/1‑149 (MQ=255) tttCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGatgatg > 3:42649/1‑148 (MQ=255) cttcgttcgCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGatgatg > 3:21479/2‑148 (MQ=255) cgttcgCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGACTTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGaa < 1:94186/148‑1 (MQ=255) ttcgCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCAc > 4:91527/1‑149 (MQ=255) aTTGATCAGGATTTGTGCAATCGTTTTCCGAAAGCGTTGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTAGAGATAATCAATGA‑TTGTTTTCGGCAGCTGCGGAACACCTTTGTTTACCTTGATCGATGATGAAGCACT‑CTGaaaaa < 3:360974/149‑1 (MQ=255) aTTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGaaaaa < 2:300651/149‑1 (MQ=255) aTTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGaaaaa < 8:62070/149‑1 (MQ=255) tCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCACAGGTCTTCGAGAAAAACAATGA‑TTTTTTACGGCAGATGCGGAACACATTTGTTTACCTTGATCCATAATGAAGAAAT‑ATGAAAAAtc > 1:109049/1‑147 (MQ=255) tCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTGGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCAGT‑CTGAAAAATGaa < 4:42649/148‑1 (MQ=255) tCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATg > 8:243807/1‑146 (MQ=255) tCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGaa < 7:243807/148‑1 (MQ=255) tCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAg > 1:112349/1‑149 (MQ=255) gatgATTTCTGCAATTGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGTTCCCCGTCTC‑‑TTCTTCGAGATTATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATTGATGATGTAGCACT‑CTGAAAAATGAAg < 2:109049/145‑1 (MQ=255) aGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGcc > 2:62301/1‑149 (MQ=255) gATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGccct > 1:242720/1‑149 (MQ=255) aTTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGccctc < 2:242720/149‑1 (MQ=255) tGCAATCGTTTTCCGAAAGCGATGCGTATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGATATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGt < 3:91527/149‑1 (MQ=255) cAATCGTTTTCCGAAAGCGATGCCGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCTAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTa < 6:364697/148‑1 (MQ=255) aaTCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGaaaaa < 4:153671/131‑1 (MQ=255) aaTCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGaaaaa > 3:153671/1‑131 (MQ=255) aaTCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAg > 3:128637/1‑148 (MQ=255) tCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAg < 5:291623/149‑1 (MQ=255) ttttCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTc > 3:307806/1‑149 (MQ=255) ttttCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTc > 8:347894/1‑149 (MQ=255) gAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACg > 6:295433/1‑147 (MQ=255) aaaGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACTCCTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTa < 1:62301/149‑1 (MQ=255) aaaGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACg > 2:162069/1‑146 (MQ=255) cGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAgagcggagcg < 3:295199/149‑1 (MQ=255) ggAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTtct < 5:295433/148‑1 (MQ=255) tCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGaa < 3:221567/101‑1 (MQ=255) tCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGaa > 4:221567/1‑101 (MQ=255) gATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTaa > 3:236412/1‑149 (MQ=255) aC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATTAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCAc < 5:380650/148‑1 (MQ=255) aC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCAc < 6:317414/148‑1 (MQ=255) gaTAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTa < 4:21479/149‑1 (MQ=255) aTAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAg < 4:128637/149‑1 (MQ=255) aaTCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAgg > 6:58903/1‑148 (MQ=255) aTCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTtct < 4:286856/114‑1 (MQ=255) aTCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTa < 2:112349/149‑1 (MQ=255) gA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTAgagg > 2:301552/1‑147 (MQ=255) gA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTAgagg > 7:225470/1‑147 (MQ=255) gA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTAgagg > 7:250136/1‑147 (MQ=255) aaaggtgtTCCGCAGCTGCCGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTCTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTAgagg > 2:288916/5‑147 (MQ=255) ttGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAgagcgga < 6:362560/99‑1 (MQ=255) | ATAGGAATGGTGGCCGAACAGCAGACGGATCAGGTCAATATTTCGTTCGCTCCGATTGATCAGGATTTCTGCAATCGTTTTCCGAAAGCGATGCGGATGGAGGCGCTCGATCCCCGTCAC‑‑TTCTTCGAGATAATCAATGA‑TTGTGTTCGGCAGCTGCGGAACACCTTTGGTTACCTTGATCGATGATGAAGCACT‑CTGAAAAATGAAGCCCTCCCTGTAGAAGGTCCACGTAGAGCGGAGCGTTCTCAGAGATTTAATGGTTTCACCAACAAATTTAGGTAGAGG > NC_002947/2223221‑2223505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |