Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,237,678 C→G 16.3% A302A (GCG→GCC) ‡ PP_1975 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,237,6780CG16.3% 132.1 / 17.7 55A302A (GCG→GCC) ‡PP_1975EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base G (4/5);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

AGGCAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAG  >  NC_002947/2237538‑2237825
                                                                                                                                             |                                                                                                                                                   
aGGCAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑GCGCAGGTa                                                                                                                                             >  5:362115/1‑149 (MQ=255)
aGGCAAAACCCCAGCGCCCATAGTACCATGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTa                                                                                                                                             <  6:350247/149‑1 (MQ=255)
  gCAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTa                                                                                                                                             >  8:286222/1‑147 (MQ=255)
  gCAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTT‑TAAACCATTACCTGCCGCCAgtaacg                                                                                                                                           >  7:101532/1‑144 (MQ=255)
   cAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑GCGCAGGTACAg                                                                                                                                          <  5:154116/149‑1 (MQ=255)
   cAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑GCGCAGGTACAg                                                                                                                                          <  7:75107/149‑1 (MQ=255)
   cAAAACCCCAGCCCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAg                                                                                                                                          <  6:88256/149‑1 (MQ=255)
    aaaaCCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGc                                                                                                                                         >  4:95162/1‑149 (MQ=255)
    aaaaCCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGc                                                                                                                                         >  4:287667/1‑149 (MQ=255)
     aaaCCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑GCGCAGGTACAggg                                                                                                                                        <  6:362115/149‑3 (MQ=255)
     aaaCCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCg                                                                                                                                        <  5:331904/149‑1 (MQ=255)
        ccccAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTa                                                                                                                                     <  4:62478/149‑1 (MQ=255)
        ccccAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTa                                                                                                                                     <  3:95162/149‑1 (MQ=255)
                      gTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑GCGCAGGTACAgg                                                                                                                                         <  2:166955/131‑2 (MQ=255)
                      gTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑GCGCAGGTACAgg                                                                                                                                         >  1:166955/1‑130 (MQ=255)
                         ccTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGcc                                                                                                                     <  7:286222/148‑1 (MQ=255)
                                        ccTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGTTAGATCGAGCCGTACAGGCCCACAcc                                                                                                      >  3:185235/1‑148 (MQ=255)
                                         cTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCa                                                                                                    >  3:321731/1‑149 (MQ=255)
                                                aGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCa                                                                                              <  1:6374/148‑1 (MQ=255)
                                                       gCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCg                                                                                      <  5:207149/149‑1 (MQ=255)
                                                         aCCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGAt                                                                                    >  3:71125/1‑149 (MQ=255)
                                                             gCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTc                                                                                <  4:321731/149‑1 (MQ=255)
                                                                 gATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACa                                                                            <  3:287667/149‑1 (MQ=255)
                                                                  agcAGGAACAGCCGCGGAATACCCATCCACATGACCACCTCCCCAATCTGCAAGGCGTTGGAACCCTGAACCTG‑CGCCAGGTACAGCGGGAGCAGATAGATCGCCCCGTACAGGCCCACACCCCTGCCCAGGCGGGCGATGCTCGACAg                                                                           <  3:217000/147‑1 (MQ=255)
                                                                               gCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCgg                                                              >  6:219878/1‑149 (MQ=255)
                                                                                cGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCg                                                               <  4:71125/147‑1 (MQ=255)
                                                                                       cccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGc                                                                >  4:53456/1‑139 (MQ=255)
                                                                                       cccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGc                                                                <  3:53456/139‑1 (MQ=255)
                                                                                       cccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAg                                                      <  3:78941/149‑1 (MQ=255)
                                                                                        ccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCATATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGACTGGCGATTCTCGACAGGCCAAAGTTGCGGTTGc                                                          <  7:234891/144‑1 (MQ=255)
                                                                                        ccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGACTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGc                                                          >  8:234891/1‑144 (MQ=255)
                                                                                                aTGATCACCTCGCCAATCTGGAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACCCCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTAGCGGTTGCCGAGGCTACGCAg                                             >  5:42327/1‑149 (MQ=255)
                                                                                                   aTCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGTTAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGtt                                          <  4:185235/149‑1 (MQ=255)
                                                                                                   aTCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGtt                                          <  2:40825/149‑1 (MQ=255)
                                                                                                      aCCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGAt                                       >  7:8074/1‑149 (MQ=255)
                                                                                                       ccTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATc                                      <  1:3746/149‑1 (MQ=255)
                                                                                                         tCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAg                                    >  7:234652/1‑149 (MQ=255)
                                                                                                             cAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTg                                                                 <  5:199699/116‑1 (MQ=255)
                                                                                                             cAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTg                                                                 >  6:199699/1‑116 (MQ=255)
                                                                                                                      aGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTg                       <  5:162608/149‑1 (MQ=255)
                                                                                                                      aGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTg                       <  5:219878/149‑1 (MQ=255)
                                                                                                                      aGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTg                       >  6:162608/1‑149 (MQ=255)
                                                                                                                      aGGCGTTGTAACCCTGTACCTG‑CGCCA‑GTACAGCGGTAGCAGATATATAGAGCCGTACAGGCCCACACCCATGCCCCGGCTTGCGATTCACGACATGCCAAATTTGCGGTTGCCGAGGATACCGATGTTTATAAGCGGGGGCGGCTTgg                      >  4:62987/1‑149 (MQ=255)
                                                                                                                      aGGCGTTGTAACCCTGTACCTG‑CGCCA‑GTACAGCGGTAGCAGATAGATAGAGCCGTACAGGCCCACACCCATGCCCATGCTGGCGATGCTAGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCATGTTGAAAAGCGGGTGAGGCTTg                       >  4:68024/1‑148 (MQ=255)
                                                                                                                        gCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTgg                      >  1:68663/1‑148 (MQ=255)
                                                                                                                          gTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCgg                                 >  4:332406/1‑135 (MQ=255)
                                                                                                                          gTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCgg                                 <  3:332406/135‑1 (MQ=255)
                                                                                                                          gTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGaa                    >  4:212292/1‑148 (MQ=255)
                                                                                                                              tAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTg               >  1:198809/1‑149 (MQ=255)
                                                                                                                                  ccTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAg            >  5:208022/1‑148 (MQ=255)
                                                                                                                                   cTGTACCTG‑GCGCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCt                         >  2:350582/1‑134 (MQ=255)
                                                                                                                                   cTGTACCTG‑GCGCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCt                         <  1:350582/134‑1 (MQ=255)
                                                                                                                                   cTGTACCTG‑GCGCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGa          >  6:252084/1‑149 (MQ=255)
                                                                                                                                    tGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGTGCCGTACACGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGATGCTTGGAAAACTGCAGGAt         >  1:258354/1‑149 (MQ=255)
                                                                                                                                       aCCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGAc      >  4:177269/1‑149 (MQ=255)
                                                                                                                                             cgcCA‑GTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAg  <  3:62987/147‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
AGGCAAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTG‑CGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAG  >  NC_002947/2237538‑2237825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑