Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,241,291 C→G 10.7% intergenic (+38/‑243) gltX → / → PP_t25 glutamate‑‑tRNA ligase/tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,241,2910CG10.7% 128.0 / 7.6 47intergenic (+38/‑243)gltX/PP_t25glutamate‑‑tRNA ligase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (13/29);  new base G (2/3);  total (15/32)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

CCGACCTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCC  >  NC_002947/2241150‑2241439
                                                                                                                                             |                                                                                                                                                     
ccGACCTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATg                                                                                                                                                <  2:6507/149‑1 (MQ=255)
 cGACCTGACGCACTACCGCCTGGGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGc                                                                                                                                               <  5:315398/149‑1 (MQ=255)
     cTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATg                                                                                                                                           <  6:7266/149‑1 (MQ=255)
       gACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGt                                                                                                                                         <  7:151526/149‑1 (MQ=255)
        aCGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGt                                                                                                                                         >  3:350066/1‑148 (MQ=255)
          gcgcTACCGCCTGCGCCAGGCTCTGGTCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGAACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTaaa                                                                                                                                      <  3:228116/149‑1 (MQ=255)
          gcgcTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGAtattgaa                                                                                                                                      >  8:24419/1‑143 (MQ=255)
          gcgcTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGAtattgaa                                                                                                                                      >  8:30170/1‑143 (MQ=255)
             cTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTc                                                                                                                                   <  3:138312/149‑1 (MQ=255)
                ccGCCTGCGCCAGGCTCTGGACCTGGTGGGGGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGATAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCGCGCGAATGCAATGGTAAATTCaa                                                                                                                                 <  5:52368/148‑1 (MQ=255)
                ccGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAat                                                                                                                                <  4:350066/149‑1 (MQ=255)
                   ccTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                             <  3:364785/149‑1 (MQ=255)
                             gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGcgg                                                                                                                   <  7:24419/149‑3 (MQ=25)
                             gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGcgg                                                                                                                   <  7:30170/149‑3 (MQ=25)
                                 tGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCAc                                                                                                               >  6:290108/1‑149 (MQ=255)
                                   gACCTGCTGGGTGGGGTGTCGTAGAAAGAAAACAAAGAGGGGGTAAAGCTGTTGGCTTCCATCGCCTGATTGAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCGGCGAATGCTATATTGAATTTACCATTGCATTCGCGGGCa                                                                                                                <  3:242731/146‑1 (MQ=21)
                                    aCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGcc                                                                                                            <  5:290108/149‑1 (MQ=255)
                                     ccTGCTTGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAt                                                                                                                                            <  8:78614/116‑1 (MQ=255)
                                      ctgctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGGCCCCGAAAGGAAATGTAAATTAAATTTATCATTAGCCGGCACGcccc                                                                                                          >  4:205539/1‑148 (MQ=255)
                                         ctgGGTGGGGTGGCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGc                                                                                                         <  7:127817/147‑1 (MQ=255)
                                            ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCca                                                                                                    <  3:125254/149‑1 (MQ=255)
                                             gtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATgg                                                                                                                                          >  8:239081/1‑110 (MQ=255)
                                             gtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATgg                                                                                                                                          <  7:239081/110‑1 (MQ=255)
                                               ggggtgTCGAAGAAAGAAACCAAAGAGGGGGAAAAGCTGGGGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAg                                                                                                 <  3:42111/149‑1 (MQ=255)
                                                 ggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGc                                                                                               <  1:58295/149‑1 (MQ=255)
                                                     tCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATCGTAAAATCAATATCGCAATAGCCGGCACGCCCGCGCCCACAAGCCaa                                                                                            >  4:237986/1‑148 (MQ=255)
                                                                    aaaGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGTTTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGGCCCCGCGGGGAAGGGGAAAATTACTATTGCCTTCGGCGGCTAGCCCCGGCCCCCGCGGCGACGAAGCCCC‑aaaacaa                                                                           >  8:208542/1‑146 (MQ=255)
                                                                      tgagTGGGAAAAGCTGGTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATTGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCg                                                                         <  3:237986/148‑1 (MQ=255)
                                                                             gAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑aaaaaa                                                                            >  1:250121/1‑139 (MQ=255)
                                                                             gAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑aaaaaa                                                                            <  2:250121/139‑1 (MQ=255)
                                                                               aaaGCTGTTGGCTTCCATCGCTTGATTAAGATTTGCACATAACCTGTGGTAGCGGGGGTGCCCGCTAATGAAATGTTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAggg                                                                 <  8:129869/148‑1 (MQ=255)
                                                                                aaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCg                                                               <  7:342710/149‑1 (MQ=255)
                                                                                                   ccTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCtgt                                            <  2:305064/149‑1 (MQ=255)
                                                                                                     tGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTTAATTAAATATAGCATTAGGCCGCCCCCCCGCACCCCAAGGCCAACTAAACCCCAAAAAAAACGGGGTGGGGC‑GTATAGGATTGTTATgtgtt                                           >  8:290687/1‑147 (MQ=255)
                                                                                                      gATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTAAATATCGCATTCGCCGGCACGCCCGCTCCCCCAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATcgttga                                          >  7:270103/1‑143 (MQ=255)
                                                                                                       aTTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAAGGTAAATTAAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGGAAGTGATTGTTATCTGTGaa                                         >  3:263933/1‑148 (MQ=255)
                                                                                                          cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCCCCCAATGCAAAGGTAAATTAAATAAACCAATAGCCGGCAACCCCGCAACCACAAGCCAAAAAAACCCC‑AAAAAACCGGGGGTAGGCGGGAACTGTATGGTATATGTGaaaaa                                      >  4:232858/1‑148 (MQ=255)
                                                                                                                tGCACCTAACCTGTGGGAGCGCGCGTGCCCGCGAATGCAATTGTTAATTCAATATCGCATGCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                <  6:70737/148‑1 (MQ=255)
                                                                                                                tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGTTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                <  5:206679/148‑1 (MQ=255)
                                                                                                                tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                <  3:185923/148‑1 (MQ=255)
                                                                                                                         ccATTGGGAGCGGGCGTGCCCGCTAATGAAATGTTAATTTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATAttttt                      <  7:144216/149‑1 (MQ=255)
                                                                                                                                    gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                <  2:358529/128‑1 (MQ=255)
                                                                                                                                    gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                >  1:358529/1‑128 (MQ=255)
                                                                                                                                     ggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCAAAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGttt          <  7:290687/149‑1 (MQ=255)
                                                                                                                                     ggCGTGCCCGCGAATCCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGaaaaa                                      <  6:292066/121‑1 (MQ=255)
                                                                                                                                     ggCGTGCCCGCGAATCCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGaaaaa                                      >  5:292066/1‑121 (MQ=255)
                                                                                                                                       cGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa        <  2:239420/149‑1 (MQ=255)
                                                                                                                                            ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTg                                                    <  4:339242/100‑1 (MQ=255)
                                                                                                                                            ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTg                                                    >  3:339242/1‑100 (MQ=255)
                                                                                                                                            ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc   >  1:101798/1‑149 (MQ=255)
                                                                                                                                             cgcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc  <  2:101798/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
CCGACCTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCC  >  NC_002947/2241150‑2241439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑