Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,241,300 A→G 8.0% intergenic (+47/‑234) gltX → / → PP_t25 glutamate‑‑tRNA ligase/tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,241,3000AG 8.0% 136.1 / 3.1 51intergenic (+47/‑234)gltX/PP_t25glutamate‑‑tRNA ligase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base A (16/30);  new base G (2/2);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

CTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCC  >  NC_002947/2241155‑2241447
                                                                                                                                                 |                                                                                                                                                     
cTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATg                                                                                                                                                    <  6:7266/149‑1 (MQ=255)
  gACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGt                                                                                                                                                  <  7:151526/149‑1 (MQ=255)
   aCGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGt                                                                                                                                                  >  3:350066/1‑148 (MQ=255)
     gcgcTACCGCCTGCGCCAGGCTCTGGTCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGAACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTaaa                                                                                                                                               <  3:228116/149‑1 (MQ=255)
     gcgcTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGAtattgaa                                                                                                                                               >  8:30170/1‑143 (MQ=255)
     gcgcTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGAtattgaa                                                                                                                                               >  8:24419/1‑143 (MQ=255)
        cTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTc                                                                                                                                            <  3:138312/149‑1 (MQ=255)
           ccGCCTGCGCCAGGCTCTGGACCTGGTGGGGGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGATAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCGCGCGAATGCAATGGTAAATTCaa                                                                                                                                          <  5:52368/148‑1 (MQ=255)
           ccGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAat                                                                                                                                         <  4:350066/149‑1 (MQ=255)
              ccTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                                      <  3:364785/149‑1 (MQ=255)
                        gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGC‑ATTCGcgg                                                                                                                           <  7:30170/149‑3 (MQ=25)
                        gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGC‑ATTCGcgg                                                                                                                           <  7:24419/149‑3 (MQ=25)
                            tGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCAc                                                                                                                       >  6:290108/1‑149 (MQ=255)
                              gACCTGCTGGGTGGGGTGTCGTAGAAAGAAAACAAAGAGGGGGTAAAGCTGTTGGCTTCCATCGCCTGATTGAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCGGCGAATGCTATATTGAATTTACCATTGC‑ATTCGCGGGCa                                                                                                                        <  3:242731/146‑1 (MQ=21)
                               aCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGcc                                                                                                                    <  5:290108/149‑1 (MQ=255)
                                ccTGCTTGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAt                                                                                                                                                     <  8:78614/116‑1 (MQ=255)
                                 ctgctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGGCCCCGAAAGGAAATGTAAATTAAATTTATC‑ATTAGCCGGCACGcccc                                                                                                                  >  4:205539/1‑148 (MQ=255)
                                    ctgGGTGGGGTGGCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGc                                                                                                                 <  7:127817/147‑1 (MQ=255)
                                       ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCca                                                                                                            <  3:125254/149‑1 (MQ=255)
                                        gtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATgg                                                                                                                                                   >  8:239081/1‑110 (MQ=255)
                                        gtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATgg                                                                                                                                                   <  7:239081/110‑1 (MQ=255)
                                          ggggtgTCGAAGAAAGAAACCAAAGAGGGGGAAAAGCTGGGGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAg                                                                                                         <  3:42111/149‑1 (MQ=255)
                                            ggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGc                                                                                                       <  1:58295/149‑1 (MQ=255)
                                                tCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATCGTAAAATCAATATCGC‑AATAGCCGGCACGCCCGCGCCCACAAGCCaa                                                                                                    >  4:237986/1‑148 (MQ=255)
                                                               aaaGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGTTTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGGCCCCGCGGGGAAGGGGAAAATTACTATTGC‑CTTCGGCGGCTAGCCCCGGCCCCCGCGGCGACGAAGCCCC‑aaaacaa                                                                                   >  8:208542/1‑146 (MQ=255)
                                                                 tgagTGGGAAAAGCTGGTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATTGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCg                                                                                 <  3:237986/148‑1 (MQ=255)
                                                                        gAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑aaaaaa                                                                                    >  1:250121/1‑139 (MQ=255)
                                                                        gAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑aaaaaa                                                                                    <  2:250121/139‑1 (MQ=255)
                                                                          aaaGCTGTTGGCTTCCATCGCTTGATTAAGATTTGCACATAACCTGTGGTAGCGGGGGTGCCCGCTAATGAAATGTTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAggg                                                                         <  8:129869/148‑1 (MQ=255)
                                                                           aaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCg                                                                       <  7:342710/149‑1 (MQ=255)
                                                                                              ccTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCtgt                                                    <  2:305064/149‑1 (MQ=255)
                                                                                                tGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTTAATTAAATATAGC‑ATTAGGCCGCCCCCCCGCACCCCAAGGCCAACTAAACCCCAAAAAAAACGGGGTGGGGC‑GTATAGGATTGTTATgtgtt                                                   >  8:290687/1‑147 (MQ=255)
                                                                                                 gATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTAAATATCGC‑ATTCGCCGGCACGCCCGCTCCCCCAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATcgttga                                                  >  7:270103/1‑143 (MQ=255)
                                                                                                  aTTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAAGGTAAATTAAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGGAAGTGATTGTTATCTGTGaa                                                 >  3:263933/1‑148 (MQ=255)
                                                                                                     cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCCCCCAATGCAAAGGTAAATTAAATAAACC‑AATAGCCGGCAACCCCGCAACCACAAGCCAAAAAAACCCC‑AAAAAACCGGGGGTAGGCGGGAACTGTATGGTATATGTGaaaaa                                              >  4:232858/1‑148 (MQ=255)
                                                                                                           tGCACCTAACCTGTGGGAGCGCGCGTGCCCGCGAATGCAATTGTTAATTCAATATCGC‑ATGCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                        <  6:70737/148‑1 (MQ=255)
                                                                                                           tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGTTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                        <  5:206679/148‑1 (MQ=255)
                                                                                                           tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                        <  3:185923/148‑1 (MQ=255)
                                                                                                                    ccATTGGGAGCGGGCGTGCCCGCTAATGAAATGTTAATTTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATAttttt                              <  7:144216/149‑1 (MQ=255)
                                                                                                                               gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                        <  2:358529/128‑1 (MQ=255)
                                                                                                                               gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                        >  1:358529/1‑128 (MQ=255)
                                                                                                                                ggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCAAAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGttt                  <  7:290687/149‑1 (MQ=255)
                                                                                                                                ggCGTGCCCGCGAATCCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGaaaaa                                              <  6:292066/121‑1 (MQ=255)
                                                                                                                                ggCGTGCCCGCGAATCCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGaaaaa                                              >  5:292066/1‑121 (MQ=255)
                                                                                                                                  cGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa                <  2:239420/149‑1 (MQ=255)
                                                                                                                                       ccGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTg                                                            <  4:339242/100‑1 (MQ=255)
                                                                                                                                       ccGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTg                                                            >  3:339242/1‑100 (MQ=255)
                                                                                                                                       ccGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc           >  1:101798/1‑149 (MQ=255)
                                                                                                                                        cgcgAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc          <  2:101798/149‑1 (MQ=255)
                                                                                                                                         gcgAATGCAATGGTAAATTCAATATCGCGATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc           >  1:22365/1‑148 (MQ=255)
                                                                                                                                         gcgAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc           <  2:22365/147‑1 (MQ=255)
                                                                                                                                                 aaTGGTAAATTCAATATCGC‑ATTCGCCGGCACGCACGCTCCCACAGGCCAACTAAGCCCC‑AAAAAAACGGCGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc  >  8:39765/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CTGACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGC‑ATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCC‑AAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCC  >  NC_002947/2241155‑2241447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑