Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,246,241	C→T	9.2%	intergenic (+30/+61)	ibpA → / ← PP_1983	small heat shock protein IbpA/PAS/PAC/GAF sensor‑containing diguanylate cyclase/phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,246,241	0	C	T	9.2%	180.0 / 6.7	65	intergenic (+30/+61)	ibpA/PP_1983	small heat shock protein IbpA/PAS/PAC/GAF sensor‑containing diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base T (3/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01

AAGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTACAGAT  >  NC_002947/2246098‑2246387                                                                                                                                                |                                                                                                                                                     aaGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAgg                                                                                                                                                 >  1:192195/1‑149 (MQ=255)      cgccgcCCGGGCCAATGGCCGGCTCAGCATCGACCTGCTGCGCATCCTCCCTGAAGAAGCCACGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGGAACGAAAAAAAGGGCACCTCCAGAGGTGccc                                                                                                                                            <  4:195751/149‑1 (MQ=255)      cgccgGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGccc                                                                                                                                            >  4:120201/1‑149 (MQ=255)       gccgGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑t                                                                                                                                         <  6:199892/149‑1 (MQ=255)           gCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑ttttt                                                                                                                                     <  6:249632/149‑1 (MQ=255)           gCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑ttttt                                                                                                                                     <  2:192195/149‑1 (MQ=255)            ccTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTc                                                                                                                                    <  4:30133/149‑1 (MQ=255)             cTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCg                                                                                                                                   >  3:259846/1‑149 (MQ=255)                gCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCTGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGt                                                                                                                                  <  4:355971/147‑1 (MQ=255)                gCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACG‑‑AAAAAGGGCACCTCTGGAGGTGCCC‑‑tttttttcgtt                                                                                                                               <  6:38286/148‑7 (MQ=38)                  cAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCtt                                                                                                                              >  1:21821/1‑149 (MQ=255)                    aTGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTc                                                                                                                                    >  6:132639/1‑141 (MQ=255)                    aTGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTc                                                                                                                                    <  5:132639/141‑1 (MQ=255)                      ggCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCa                                                                                                                          >  8:105077/1‑149 (MQ=255)                       gCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCAc                                                                                                                         <  1:208649/149‑1 (MQ=255)                           gCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAg                                                                                                                     >  1:234511/1‑149 (MQ=255)                              cAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCTGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGAAGTcc                                                                                                                  >  5:317050/1‑149 (MQ=255)                                 cATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCcgc                                                                                                               >  7:144878/1‑149 (MQ=255)                                     gACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTa                                                                                                            <  5:88591/148‑1 (MQ=255)                                     gACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTa                                                                                                            <  5:63319/148‑1 (MQ=255)                                      aCCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAgg                                                                                                          <  4:259846/149‑1 (MQ=255)                                               gcATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGcc                                                                                                 <  2:234511/149‑1 (MQ=255)                                                 aTCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGt                                                                                               >  1:285634/1‑149 (MQ=255)                                                  tCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTc                                                                                              <  8:144878/149‑1 (MQ=255)                                                    gTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGTCCGTCAt                                                                                            <  2:21821/149‑1 (MQ=255)                                                       ccTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGc                                                                                          >  7:95324/1‑148 (MQ=255)                                                                   aaggccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTg                                                                             >  1:357593/1‑149 (MQ=255)                                                                      gccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCagag                                                                                                                                                  <  1:107346/79‑1 (MQ=255)                                                                      gccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCagag                                                                                                                                                  >  2:107346/1‑79 (MQ=255)                                                                           gcgcATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑‑TTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGgcgc                                                                      <  6:195914/147‑1 (MQ=255)                                                                           gcgcATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑‑TTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGgcgc                                                                      >  5:195914/1‑147 (MQ=255)                                                                                ttCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGcc                                                                                                 >  4:140317/1‑116 (MQ=255)                                                                                ttCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGcc                                                                                                 <  3:140317/116‑1 (MQ=255)                                                                                         aTGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTg                                                       >  8:18021/1‑149 (MQ=255)                                                                                         aTGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTg                                                       >  3:240529/1‑149 (MQ=255)                                                                                              gAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCg                                                    <  3:181867/147‑1 (MQ=255)                                                                                              gAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCg                                                    >  4:181867/1‑147 (MQ=255)                                                                                              gAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGa                                                   >  3:238132/1‑148 (MQ=255)                                                                                                      aGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcgg                                           >  7:285241/1‑148 (MQ=255)                                                                                                        cgcTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaa                                        <  5:266254/149‑1 (MQ=255)                                                                                                           tGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaacgg                                     >  6:81706/1‑149 (MQ=255)                                                                                                             aaCTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCTAGCAACATCTGCCGAAACTCcggtaacgg                                     <  2:357593/147‑1 (MQ=255)                                                                                                                    cGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGc                                                               >  5:225099/1‑114 (MQ=255)                                                                                                                    cGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGc                                                               <  6:225099/114‑1 (MQ=255)                                                                                                                    cGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCAC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTACAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCg                            >  3:73093/1‑149 (MQ=255)                                                                                                                            aaCGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCa                    <  5:191009/149‑1 (MQ=255)                                                                                                                             aCGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCtgtg                      >  6:304815/1‑146 (MQ=255)                                                                                                                             aCGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCtgtg                      >  2:8184/1‑146 (MQ=255)                                                                                                                               gAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGAt                 <  7:105077/149‑1 (MQ=255)                                                                                                                               acgaaaaaGGGCACCTCTGGAGGTGCCCTTTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGc                              >  1:54537/4‑138 (MQ=38)                                                                                                                               acgaaaaaGGGCACCTCTGGAGGTGCCCTTTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGc                              <  2:54537/135‑1 (MQ=38)                                                                                                                                      aGGGCACCTCTGGAGGTGCCCTTTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGt                                                                                   >  3:7169/1‑78 (MQ=25)                                                                                                                                      aGGGCACCTCTGGAGGTGCCCTTTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGt                                                                                   <  4:7169/78‑1 (MQ=25)                                                                                                                                      aGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTg          <  8:285241/149‑1 (MQ=255)                                                                                                                                       gggCACCTCTGGAGGTGCCCTTTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCACCATCTGCCGCAACACCGGTAACGGTAATGGc                              >  8:161908/1‑130 (MQ=255)                                                                                                                                       gggCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCcgcg                                                                                                              >  6:230582/1‑48 (MQ=255)                                                                                                                                       gggCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCcgcg                                                                                                              <  5:230582/48‑1 (MQ=255)                                                                                                                                       gggCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTgg         <  2:285634/149‑1 (MQ=255)                                                                                                                                        ggCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaacg                                      <  8:345635/119‑1 (MQ=255)                                                                                                                                        ggCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaacg                                      >  7:345635/1‑119 (MQ=255)                                                                                                                                         gCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGt                                                                                               >  4:102017/1‑61 (MQ=255)                                                                                                                                         gCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGt                                                                                               <  3:102017/61‑1 (MQ=255)                                                                                                                                         gCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACCTCTGCCGAAACTCCGGTAACGGTAATGCCCGGCTGTGCAGATAACCCTGGTa       <  1:99502/149‑1 (MQ=255)                                                                                                                                             cTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTa       >  4:241191/1‑145 (MQ=255)                                                                                                                                             cTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTa       >  3:150808/1‑145 (MQ=255)                                                                                                                                               ccAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCa                    >  7:259307/1‑130 (MQ=255)                                                                                                                                               ccAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCa                    <  8:259307/130‑1 (MQ=255)                                                                                                                                                cAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTACAGAt  <  5:81706/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     AAGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCC‑‑TTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTACAGAT  >  NC_002947/2246098‑2246387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑