BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	226,226	T→A	5.4%	L151Q (CTG→CAG)	PP_0174 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	226,226	0	T	A	5.4%	331.7 / 5.5	109	L151Q (CTG→CAG)	PP_0174	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (54/49); new base A (2/4); total (56/53)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 113 total aligned reads displayed.

GCCGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAC  >  NC_002947/226078‑226374

gCCGGGCACCTGTTGCGCATGGAGGCCCTGCAGGGCAATGCTGCAGCCTGGTTAAACCTTGGCAATCAGCACAACTTCGGCAAAGGCATCGACCCTTCCAATGTGCAGGCGGCGGAGTCTTACCGCCAGCCCTTCGACAGCGGCAtgct                                                                                                                                                      >  2:325706/1‑149 (MQ=255)

     gCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATg                                                                                                                                                 >  2:342085/1‑149 (MQ=255)

      cACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgc                                                                                                                                                >  5:123727/1‑149 (MQ=255)

          tGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgcc                                                                                                                                            >  4:293402/1‑149 (MQ=255)

            ttGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgccg                                                                                                                                           <  6:123727/148‑1 (MQ=255)

            ttGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcg                                                                                                                                            <  6:248160/147‑2 (MQ=255)

            ttGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTGCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcg                                                                                                                                            <  5:311542/147‑2 (MQ=255)

                gcATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcg                                                                                                                                            <  3:32670/143‑2 (MQ=255)

                gcATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcg                                                                                                                                            >  4:32670/1‑142 (MQ=255)

                  aTGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACt                                                                                                                                    >  3:3733/1‑149 (MQ=255)

                     gcggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTggg                                                                                                                                 <  7:228363/149‑1 (MQ=255)

                     gcggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTggg                                                                                                                                 >  8:65078/1‑149 (MQ=255)

                     gcggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTgg                                                                                                                                  <  4:24706/148‑1 (MQ=255)

                     gcggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTgg                                                                                                                                  <  8:59936/148‑1 (MQ=255)

                         cccTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGAc                                                                                                                             <  8:19188/149‑1 (MQ=255)

                          ccTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGAcc                                                                                                                            >  2:270110/1‑149 (MQ=255)

                            tGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTg                                                                                                                          >  5:205401/1‑149 (MQ=255)

                                gggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTAt                                                                                                                       >  3:178234/1‑148 (MQ=255)

                                 ggaaaTGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTGGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATga                                                                                                                     <  4:3733/146‑1 (MQ=255)

                                 ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTAt                                                                                                                       >  2:81547/1‑147 (MQ=255)

                                 ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATga                                                                                                                     <  7:66618/149‑1 (MQ=255)

                                 ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATga                                                                                                                     <  4:182607/149‑1 (MQ=255)

                                 ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATga                                                                                                                     <  4:178234/149‑1 (MQ=255)

                                  gCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    <  8:252627/149‑1 (MQ=255)

                                   cAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTAt                                                                                                                       <  6:217348/145‑1 (MQ=255)

                                   cAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTAt                                                                                                                       >  5:217348/1‑145 (MQ=255)

                                     aTGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggag                                                                                                                 <  6:101555/149‑1 (MQ=255)

                                         tgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGaggaggagg                                                                                                             >  7:80001/1‑149 (MQ=255)

                                            tgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    <  8:54829/139‑1 (MQ=255)

                                              ccTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGc                                                                                                        >  7:274232/1‑149 (MQ=255)

                                              ccTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGc                                                                                                        >  4:304551/1‑149 (MQ=255)

                                               cTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGc                                                                                                        >  1:112167/1‑148 (MQ=255)

                                                 ggTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGAc                                                                                                     >  7:106006/1‑149 (MQ=255)

                                                    tCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGt                                                                                                  >  3:135553/1‑149 (MQ=255)

                                                       aCCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGAc                                                                                               >  6:136790/1‑149 (MQ=255)

                                                         cTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                             >  7:216645/1‑149 (MQ=255)

                                                         cTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                             <  6:205401/149‑1 (MQ=255)

                                                            ggCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTg                                                                                            <  3:46983/147‑1 (MQ=255)

                                                             gCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTCTGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGTAGGTGTGCGACGGTGACCTGGa                                                                                          <  7:237707/148‑1 (MQ=255)

                                                                   agcaCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAg                                                                                   >  6:102602/1‑149 (MQ=255)

                                                                     caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGAc                                                                                               >  6:179806/1‑135 (MQ=255)

                                                                     caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGAc                                                                                               <  5:179806/135‑1 (MQ=255)

                                                                     caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTg                                                                                            >  7:310448/1‑138 (MQ=255)

                                                                     caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTg                                                                                            <  8:310448/138‑1 (MQ=255)

                                                                     caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGt                                                                                 >  7:144863/1‑149 (MQ=255)

                                                                     caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGt                                                                                 <  1:270110/149‑1 (MQ=255)

                                                                        tactTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGa                                                                              >  3:347128/1‑149 (MQ=255)

                                                                           ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGCCCTGTATGAGGAGGAGGTGTGCGCCGGTGACCTGGAATGGCAGGTTGACCt                                                                           >  3:115547/1‑149 (MQ=255)

                                                                           ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCt                                                                           <  7:65078/149‑1 (MQ=255)

                                                                           ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCt                                                                           >  6:315150/1‑149 (MQ=255)

                                                                           ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCt                                                                           >  1:379796/1‑149 (MQ=255)

                                                                           ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCt                                                                           >  5:74002/1‑149 (MQ=255)

                                                                              ggCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTgg                                                                         <  8:216645/148‑1 (MQ=255)

                                                                                cAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTg                                                                       >  5:213259/1‑148 (MQ=255)

                                                                                 aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGATTGGCAGGTTGACATGGTgaa                                                                     >  4:101204/1‑147 (MQ=255)

                                                                                   aGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAg                                                                   <  2:85325/149‑1 (MQ=255)

                                                                                   aGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAg                                                                   <  1:342085/149‑1 (MQ=255)

                                                                                        tCGACCCTTCGTAGGTTCAGGCGTCGTAGTTTTACCGGCAGGGCTTCGAGCGCGGAATGCTGCATGCCGCAGCCTCACTGGGTGACGTGTATGAGGATTATGTTTGCGACGGTGACCTGTAATGGCAGGTTGACCTGGTGCAAGCCTAt                                                              <  1:325706/149‑1 (MQ=255)

                                                                                          gACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCa                                                            <  5:315150/149‑1 (MQ=255)

                                                                                           aCCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAg                                                           >  8:3248/1‑149 (MQ=255)

                                                                                              cTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                          <  4:336846/131‑1 (MQ=255)

                                                                                              cTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                          >  3:336846/1‑131 (MQ=255)

                                                                                              cTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTgg                                                        >  4:78421/1‑149 (MQ=255)

                                                                                              cTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTgg                                                        <  8:274232/149‑1 (MQ=255)

                                                                                               ttCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGt                                                       >  1:15409/1‑149 (MQ=255)

                                                                                                 ccTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGtt                                                      <  2:379796/148‑1 (MQ=255)

                                                                                                 ccTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGt                                                       <  8:106006/147‑1 (MQ=255)

                                                                                                   tATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAgg                                                                                  >  3:80103/1‑118 (MQ=255)

                                                                                                   tATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAgg                                                                                  <  4:80103/118‑1 (MQ=255)

                                                                                                     tgtgCAGGCGGCGGAGTGGTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCtt                                                   <  8:220695/147‑1 (MQ=255)

                                                                                                        gCAGGCGGCGGAGTGTTACCTGCAGGCCTTCGACCGCGGAATGCTGCATGCCGCCGCAGCACTGGGTGAACTGTATCAGATGGAGGTTTGCGACGTTCACATGAAATGCAAGGTTGACCTGGTCAAACCAAATCATTGGTTGTTGCGTg                                              >  4:194293/1‑149 (MQ=255)

                                                                                                          aGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGgc                                            >  6:382868/1‑149 (MQ=255)

                                                                                                          aGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGgc                                            >  2:226489/1‑149 (MQ=255)

                                                                                                            gcggcgGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGgcgc                                          >  6:41190/1‑149 (MQ=255)

                                                                                                            gcggcgGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGgcgc                                          >  6:83479/1‑149 (MQ=255)

                                                                                                              ggcggAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGcc                                         >  6:196742/1‑148 (MQ=255)

                                                                                                                     tgtTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAggg                                 >  4:161561/1‑149 (MQ=255)

                                                                                                                     tgtTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAgg                                  <  2:112167/148‑1 (MQ=255)

                                                                                                                         aCCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGgt                                                                                                            <  1:162814/70‑1 (MQ=255)

                                                                                                                         aCCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGgt                                                                                                            >  2:162814/1‑70 (MQ=255)

                                                                                                                           cGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGgc                           >  3:111801/1‑149 (MQ=255)

                                                                                                                           cGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGgc                           <  1:81547/149‑1 (MQ=255)

                                                                                                                             ggaGGGCTTCGACCGCGGGATGGTGGACGCCGCCGGCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGgcgc                         <  3:194293/147‑1 (MQ=255)

                                                                                                                                ggCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGtt                      >  7:119828/1‑149 (MQ=255)

                                                                                                                                 gCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACATGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTg                     >  7:191992/1‑149 (MQ=255)

                                                                                                                                   cTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                   <  3:304551/149‑1 (MQ=255)

                                                                                                                                   cTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                   <  3:345556/149‑1 (MQ=255)

                                                                                                                                   cTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                   >  4:261275/1‑149 (MQ=255)

                                                                                                                                    ttCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCg                  >  5:230727/1‑149 (MQ=255)

                                                                                                                                       gACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGttt               <  1:276245/149‑1 (MQ=255)

                                                                                                                                       gACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGttt               <  6:241612/149‑1 (MQ=255)

                                                                                                                                       caCCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGttt               >  3:141666/2‑149 (MQ=255)

                                                                                                                                        aCCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGCCCTGGTGCTAGCCTAGGAGTGGTTCTTGCGTGGCGGCGCACAGGGGGCGGCGCGTGGTCGgttg              >  4:292517/1‑148 (MQ=255)

                                                                                                                                        aCCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGtttt              <  5:136790/149‑1 (MQ=255)

                                                                                                                                        aCCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGtttt              <  8:124833/149‑1 (MQ=255)

                                                                                                                                              gCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATg        <  5:41190/149‑1 (MQ=255)

                                                                                                                                              gCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATg        <  5:83479/149‑1 (MQ=255)

                                                                                                                                                aTGCAGCATGCCGCGGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATggg      >  5:94377/1‑149 (MQ=255)

                                                                                                                                                  gctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGc     >  7:188550/1‑148 (MQ=255)

                                                                                                                                                    tgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAc  <  6:74002/149‑1 (MQ=255)

GCCGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAC  >  NC_002947/226078‑226374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`