Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,264,419	G→A	8.9%	P123P (CCG→CCA)	accD →	acetyl‑CoA carboxylase carboxyltransferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,264,419	0	G	A	8.9%	186.3 / 6.6	67	P123P (CCG→CCA)	accD	acetyl‑CoA carboxylase carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base A (3/3);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

TGCCGAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCGGTGCGCGCATGCAG  >  NC_002947/2264272‑2264566                                                                                                                                                     |                                                                                                                                                     tGCCGAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGa                                                                                                                                                     <  2:46997/149‑1 (MQ=255)  gCCGAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGAt                                                                                                                                                    >  5:244530/1‑149 (MQ=255)  gCCGAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCAAt                                                                                                                                                    >  6:310729/1‑149 (MQ=255)     gAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCAATCGt                                                                                                                                                 >  1:168220/1‑149 (MQ=255)     gAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCAATCGt                                                                                                                                                 >  1:173756/1‑149 (MQ=255)      aaCTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTg                                                                                                                                                <  8:227110/149‑1 (MQ=255)      aaCTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTg                                                                                                                                                <  8:104812/149‑1 (MQ=255)        cTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGt                                                                                                                                              <  5:187047/149‑1 (MQ=255)        cTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGt                                                                                                                                              >  3:190440/1‑149 (MQ=255)         tGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGt                                                                                                                                              >  4:160992/1‑148 (MQ=255)            gcgcTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCAATCGTGGTTAgc                                                                                                                                          <  2:168220/149‑1 (MQ=255)            gcgcTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCAATCGTGGTTAgc                                                                                                                                          <  2:173756/149‑1 (MQ=255)                   ccTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTc                                                                                                                                    >  5:118487/1‑148 (MQ=255)                       gAACCGGTCGTCCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAg                                                                                                                                 <  6:153029/147‑1 (MQ=255)                       gAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAg                                                                                                                                 <  1:16677/147‑1 (MQ=255)                           cGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCg                                                                                                                           <  4:190440/149‑1 (MQ=255)                           cGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCg                                                                                                                           >  4:369131/1‑149 (MQ=255)                             gTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTtctc                                                                                                                            >  4:240947/1‑146 (MQ=255)                             gTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTtctc                                                                                                                            <  3:240947/146‑1 (MQ=255)                             gTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGACGATCGTGGTTAGCGCCTGCGAGTTAGCGtt                                                                                                                         >  8:260892/1‑149 (MQ=255)                                     ccTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGa                                                                                                                                  >  5:124353/1‑132 (MQ=255)                                     ccTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGa                                                                                                                                  <  6:124353/132‑1 (MQ=255)                                     ccTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGggcg                                                                                                                 >  1:194700/1‑149 (MQ=255)                                     ccTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGggcg                                                                                                                 >  3:372874/1‑149 (MQ=255)                                      cTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGggcgg                                                                                                                >  3:298730/1‑149 (MQ=255)                                        gAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCt                                                                                                              >  4:149792/1‑149 (MQ=255)                                            ttCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCAt                                                                                                          >  1:128504/1‑149 (MQ=255)                                            ttCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCAt                                                                                                          >  3:88548/1‑149 (MQ=255)                                               cGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATgg                                                                                                        <  6:244530/148‑1 (MQ=255)                                                      gCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCAtc                                                                                                >  5:204274/1‑149 (MQ=255)                                                       cAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCAtcg                                                                                               >  1:187595/1‑149 (MQ=255)                                                        aagaagTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCAtcgt                                                                                              >  3:189239/1‑149 (MQ=255)                                                            agTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GCGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGc                                                                                          <  7:249599/149‑1 (MQ=255)                                                            agTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCgg                                                                                           <  2:128504/148‑1 (MQ=255)                                                            agTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGc                                                                                          <  2:194700/149‑1 (MQ=255)                                                              tACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGc                                                                                          >  8:16375/1‑147 (MQ=255)                                                                cAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAgc                                                                                      >  5:245012/1‑149 (MQ=255)                                                                  aGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAgcgc                                                                                    <  7:16375/149‑1 (MQ=255)                                                                   ggACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGTCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCt                                                                                   >  6:123000/1‑149 (MQ=255)                                                                   ggACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCt                                                                                   >  3:132217/1‑149 (MQ=255)                                                                     aCCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTc                                                                                 <  4:189239/149‑1 (MQ=255)                                                                                    cccAGAAGCAGAATGGCGAAAAGGACTCGCTGATCTCCATCCCCCTGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCaa                                                                  <  1:316336/147‑1 (MQ=255)                                                                                      cAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTa                                                               >  2:255311/1‑149 (MQ=255)                                                                                           gCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCCTGC‑GGCCAACTATGccc                                                          >  7:160696/1‑149 (MQ=255)                                                                                             aGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTg                                                        <  3:339133/149‑1 (MQ=255)                                                                                              gACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGTCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTgg                                                       <  5:123000/149‑1 (MQ=255)                                                                                              gACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTgg                                                       <  1:255311/149‑1 (MQ=255)                                                                                                     gAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCg                                                >  1:24503/1‑149 (MQ=255)                                                                                                           gACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGccc                                          <  4:88548/149‑1 (MQ=255)                                                                                                              gctcTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAAt                                       <  7:260892/146‑1 (MQ=255)                                                                                                              gcgcTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAAt                                       >  6:1306/1‑149 (MQ=255)                                                                                                                 cTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGt                                    <  4:298730/149‑1 (MQ=255)                                                                                                                    aTCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCAGGCCAACTATGCCCTGGAAAACCGCTg                                             <  1:209960/138‑1 (MQ=255)                                                                                                                    aTCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTg                                             >  2:209960/1‑137 (MQ=255)                                                                                                                    aTCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGttt                                  <  4:372874/148‑1 (MQ=255)                                                                                                                         cAT‑GAGCGGCACCCTGATGGGCATGCCAATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGc                                <  5:310729/145‑1 (MQ=255)                                                                                                                          aT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTtctc                           >  1:201943/1‑149 (MQ=255)                                                                                                                                 ggCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTc                     <  6:204274/149‑1 (MQ=255)                                                                                                                                 ggCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTc                     <  3:160992/149‑1 (MQ=255)                                                                                                                                    aCCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCAGGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCggg                  >  2:238295/1‑149 (MQ=255)                                                                                                                                      ccTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCg                >  8:332042/1‑149 (MQ=255)                                                                                                                                           tGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCGGTgc            <  7:216333/148‑1 (MQ=255)                                                                                                                                             ggCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCGGTgcgc          <  2:187595/148‑1 (MQ=255)                                                                                                                                                aTGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGttt                                  >  7:8853/1‑121 (MQ=255)                                                                                                                                                aTGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGttt                                  <  8:8853/121‑1 (MQ=255)                                                                                                                                                   ccGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCGGTGCGCGCATGc    <  4:132217/148‑1 (MQ=255)                                                                                                                                                    cGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCGGTGCGCGCATGCAg  <  6:118487/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TGCCGAACTGGGCGCTGACCTGGAACCGGTCGACCGCCTGAAGTTCCGTGATGGCAAGAAGTACAAGGACCGCCTGACCGCTGCGCAGAAGCAGACTGGCGAAAAGGACGCGCTGATCTCCAT‑GAGCGGCACCCTGATGGGCATGCCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGC‑GGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTTCTCCGCTTCGGGCGGTGCGCGCATGCAG  >  NC_002947/2264272‑2264566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑