Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,266,201	T→C	5.9%	W421R (TGG→CGG)	folC →	bifunctional folypolyglutamate synthase/dihydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,266,201	0	T	C	5.9%	200.6 / 2.1	68	W421R (TGG→CGG)	folC	bifunctional folypolyglutamate synthase/dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base C (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GCCTCGTCCTTGGCGAACCTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACGAGATGCGCCAGG  >  NC_002947/2266057‑2266349                                                                                                                                                   |                                                                                                                                                     gCCTCGTCCTTGGCGAACCTCGGGGCCACGGTGTAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGc                                                                                                                                                   <  6:188097/148‑1 (MQ=255)        ccTTGGCGAACCTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATgg                                                                                                                                                    <  8:249495/140‑1 (MQ=255)        ccTTGGCGAACCTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATgg                                                                                                                                                    >  7:249495/1‑140 (MQ=255)         cTTGGCGAACCTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGCGCGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCgg                                                                                                                                           >  1:88577/1‑149 (MQ=255)                   cTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCagga                                                                                                                                <  5:272593/149‑1 (MQ=255)                   cTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCagga                                                                                                                                <  3:81535/149‑1 (MQ=255)                   cTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCagga                                                                                                                                <  2:130948/149‑1 (MQ=255)                        tGCCTCGGTGAAGTCTTATGTCAGCGGCGTCGC‑AGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCATGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTg                                                                                                                           <  6:372510/149‑1 (MQ=255)                        tGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTg                                                                                                                           <  4:247390/149‑1 (MQ=255)                                tGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGAt                                                                                                                   <  4:214079/149‑1 (MQ=255)                                  aaGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCa                                                                                                                                         <  1:315464/125‑1 (MQ=255)                                  aaGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCa                                                                                                                                         >  2:315464/1‑125 (MQ=255)                                     tCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCtg                                                                                                              >  1:10482/1‑149 (MQ=255)                                      cTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCaggag                                                                                                                               >  7:198587/1‑131 (MQ=255)                                      cTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCaggag                                                                                                                               <  8:198587/131‑1 (MQ=255)                                         aTGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCt                                                                                                          >  1:359864/1‑149 (MQ=255)                                         aTGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCt                                                                                                          >  7:48867/1‑149 (MQ=255)                                          tGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTg                                                                                                         <  7:119836/149‑1 (MQ=255)                                            tCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGa                                                                                                       <  7:223703/149‑1 (MQ=255)                                            tCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGa                                                                                                       >  6:138537/1‑149 (MQ=255)                                              aGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGAt                                                                                                                   >  5:326020/1‑135 (MQ=255)                                              aGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGAt                                                                                                                   <  6:326020/135‑1 (MQ=255)                                              aGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGAt                                                                                                      >  8:292483/1‑148 (MQ=255)                                               gCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CACCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATa                                                                                                     >  6:98424/1‑148 (MQ=255)                                                  tCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAgg                                                                                                 >  3:336707/1‑149 (MQ=255)                                                    gACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAggg                                                                                                <  7:99396/148‑1 (MQ=255)                                                     aCGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGAt                                                                                              >  7:321593/1‑149 (MQ=255)                                                           gccCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAACGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGaaa                                                                                          >  3:114832/1‑148 (MQ=255)                                                           gccCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAACGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGaaa                                                                                          >  3:109604/1‑148 (MQ=255)                                                              cTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAgc                                                                                      <  5:138537/149‑1 (MQ=255)                                                                    gggCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCAtgg                                                                                >  7:42344/1‑149 (MQ=255)                                                                       cAATGTGCGCAGGCGACGGCGGATGATCAGCTCCTGCTGGTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCGGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCAtggtgg                                                                             <  6:370986/149‑1 (MQ=255)                                                                       cAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGTGTGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCAtggtg                                                                              >  1:109013/1‑149 (MQ=255)                                                                         aTGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATggtgggt                                                                           >  4:264094/1‑149 (MQ=255)                                                                          tgtgCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATggtgggtg                                                                          >  4:112879/1‑149 (MQ=255)                                                                                cAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCgctgg                                                                    <  2:88577/149‑1 (MQ=255)                                                                                       cggcggATGATCAGATCCTGCTCTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCgctggtgctggt                                                             <  8:145999/149‑1 (MQ=255)                                                                                        ggcggATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCGCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGctggtgctggtg                                                            >  2:345364/1‑149 (MQ=255)                                                                                        ggcggATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCgctggtgctggt                                                             <  5:263858/148‑1 (MQ=255)                                                                                        ggcggATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGctggtgctggtg                                                            <  7:211734/149‑1 (MQ=255)                                                                                           ggATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTCGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGgcg                                                         <  7:25711/149‑1 (MQ=255)                                                                                                atCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTgg                                                     <  8:321593/148‑1 (MQ=255)                                                                                                atCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CACCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGc                                                    <  5:98424/149‑1 (MQ=255)                                                                                                    gATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGt                                                 >  2:188977/1‑148 (MQ=255)                                                                                                       ccTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATc                                             >  2:1757/1‑149 (MQ=255)                                                                                                        ctgctgTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATc                                             <  8:42344/148‑1 (MQ=255)                                                                                                        ctgctgTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAACGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCt                                            <  4:109604/149‑1 (MQ=255)                                                                                                        ctgctgTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAACGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCt                                            <  4:114832/149‑1 (MQ=255)                                                                                                              ttcggttcgTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGc                                      >  8:284610/1‑149 (MQ=255)                                                                                                                  gttcgTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGAt                                  <  3:264094/149‑1 (MQ=255)                                                                                                                   ttcgTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTgcgc                                                                       <  8:350412/111‑1 (MQ=255)                                                                                                                   ttcgTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTgcgc                                                                       >  7:350412/1‑111 (MQ=255)                                                                                                                   ttcgTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATg                                 >  8:213523/1‑149 (MQ=255)                                                                                                                      gTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGAt                                                                                              <  2:278699/85‑1 (MQ=255)                                                                                                                      gTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAAGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGAt                                                                                              >  1:278699/1‑85 (MQ=255)                                                                                                                                gt‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGc                     >  3:213018/1‑148 (MQ=255)                                                                                                                                     cccAGGCGCTGGAATGGCTGGGGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGcc                    >  2:311078/1‑145 (MQ=255)                                                                                                                                     cccAGGCGCTGGAATGGCTGGGGCGGCACGCCCAGGAGGGTGGAATAGAAGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGcc                    <  1:311078/145‑1 (MQ=255)                                                                                                                                       cAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGAc               >  1:194699/1‑148 (MQ=255)                                                                                                                                       cAGGCGCTCGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGCATGAAACAGCGAAAGGTGGGTGCGCTGGTGATGGTGGCGCTGGCGTTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGAc               >  1:194702/1‑148 (MQ=255)                                                                                                                                        aGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACga             <  7:284610/149‑1 (MQ=255)                                                                                                                                         ggCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACgag            <  2:225400/149‑1 (MQ=255)                                                                                                                                          gcgcTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGAc               >  6:304306/1‑145 (MQ=255)                                                                                                                                          gcgcTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACgaga           <  7:292483/149‑1 (MQ=255)                                                                                                                                                gAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACGAGATgcgc      <  1:188977/148‑1 (MQ=255)                                                                                                                                                gAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACGAGATGCGcc     <  8:48867/149‑1 (MQ=255)                                                                                                                                                  aTGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCt                                        <  6:117638/112‑1 (MQ=255)                                                                                                                                                  aTGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCt                                        >  5:117638/1‑112 (MQ=255)                                                                                                                                                   tGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACGAGATGCGCCAgg  <  5:45379/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GCCTCGTCCTTGGCGAACCTCGGTGCCACGGTGAAGTCTTATGTCAGCGTCGACGC‑CGCCCTTGAAGGGCAATGTGCGCAGGCGACGGCGGATGATCAGATCCTGCTGTTCGGTTCGTTTTTCTGTGT‑CGCCCAGGCGCTGGAATGGCTGGAGCGGCACGCCCAGGAGGGTGGAATAGATGGCTGTGCTGGATAAAGGGATGAAACAGCGCATGGTGGGTGCGCTGGTGCTGGTGGCGCTGGCGGTTATCTTCCTGCCGATGCTGTTTACCCGCCAGGACGAGATGCGCCAGG  >  NC_002947/2266057‑2266349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑