| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,273,062 | C→G | 13.0% | intergenic (+27/+264) | PP_2003 → / ← PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,273,062 | 0 | C | G | 13.0% | 118.2 / 7.9 | 45 | intergenic (+27/+264) | PP_2003/PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (19/20); new base G (4/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
CGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTA > NC_002947/2272923‑2273209 | cGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCcg > 6:291509/1‑149 (MQ=255) aTGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGc > 4:107416/1‑149 (MQ=255) aTGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGc < 2:306738/149‑1 (MQ=255) aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCg < 1:373370/148‑1 (MQ=255) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAgggt < 1:159892/144‑2 (MQ=255) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAgggt > 2:159892/1‑143 (MQ=255) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGATCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGtt > 5:369812/1‑149 (MQ=255) ccAGGGTTCGCAGCCTGTGGCCCCGCCGGGCGAAGCTGCACCCATTCTGGTGCAGCAGGATGCCCAGCCTGGCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACGCTTTCGGCGGGAGGCCGGGTAAGGGCCGGttga < 8:26395/149‑3 (MQ=255) ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGtt > 2:254113/1‑147 (MQ=255) ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑a > 6:184555/1‑149 (MQ=255) gggTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCGGAAACCCCCCATTGGCCGGCCGCCCGCGCAAGGGGCGGGTG‑‑Aggg > 1:375123/1‑148 (MQ=255) cgcGCAGCCTGTGGCCCCGCCGGCCGAAGCGGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGGCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGt < 6:190371/147‑1 (MQ=255) cGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTa < 1:14858/148‑1 (MQ=255) gCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCGGGCTGCCGGCGAAAgg < 6:265975/131‑1 (MQ=255) gCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCGGGCTGCCGGCGAAAgg > 5:265975/1‑131 (MQ=255) ccTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCCGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTACACATc > 3:103167/1‑149 (MQ=255) cTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATcc > 2:106750/1‑149 (MQ=255) tgtgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAAACCACCCATTGGCCCGCAGCCCGCCAAATGGGCCGTTA‑‑AGGCTGTaaaaaaacc > 6:232259/1‑143 (MQ=255) tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCt < 3:107416/149‑1 (MQ=255) ccgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTAc < 5:184555/149‑1 (MQ=255) cgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACa < 6:65494/149‑1 (MQ=255) cgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACa < 5:291509/149‑1 (MQ=255) gCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAAACCCCCCATTCGCCGGCCGCCCCCGAAAGGGCCCGGTA‑‑AGGCTGTAAACCTGCCCGGTTACAGACGCCCCGCGAt > 3:28493/1‑148 (MQ=255) ttCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTAGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGAAGCCCCGCGCTTACTTTCt > 6:202791/1‑149 (MQ=255) cAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTg < 1:106750/148‑1 (MQ=255) cAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTg < 7:38508/148‑1 (MQ=255) cagGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCt < 4:301894/149‑1 (MQ=255) ccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATccc < 2:273973/101‑1 (MQ=255) ccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATccc > 1:273973/1‑101 (MQ=255) aGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCa < 2:375123/149‑1 (MQ=255) cggccgAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTc < 3:233335/149‑1 (MQ=255) aGGCCGCCGCCCCCTCCCTTTGATGGATCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc < 6:369812/149‑1 (MQ=255) gccgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCtt < 4:28493/149‑1 (MQ=255) cctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt > 1:298336/1‑149 (MQ=255) ctTTGATGGGTCGCCTGAACCGACCCTTTAGCCGGCATCCCGCGAAACGGCCGGTTA‑‑AGGCTGTAAACATACCCTGTTACAGACGCCCCCCGATAAATTTATTAACACCAGCTAAGTTTACCACTCATAACCATGTCATCATACCtta > 4:87660/1‑147 (MQ=255) ttGATGGGTCGCCTGAAACGCCACTTTCGCCGGCAGCCCGCCAAAGGGCCCGTTC‑‑AGGCTGTAAACAACCCCTGTTACAGACGCCCCCCCAAAACATTCTGAACACCTGCTACGTTTAACAAACAAAATCTTGTCATCATACCTTCAta > 2:122801/1‑148 (MQ=255) aaCCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGc < 2:264501/148‑1 (MQ=255) ccGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGAAAACATCCCCTGTTACAGACGCCCCGCGATATCTTTCTGAACACCTGCTACGTTTAACAGTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg > 4:216672/1‑148 (MQ=255) cGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGaa < 5:232259/149‑1 (MQ=255) ggcccTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAg > 7:247531/3‑149 (MQ=255) gACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAg > 4:144873/1‑149 (MQ=255) cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa > 5:357662/1‑149 (MQ=255) tttCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAg > 3:161518/1‑144 (MQ=255) tttCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAg < 4:161518/144‑1 (MQ=255) tttCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa > 2:117978/1‑148 (MQ=255) ccGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTa < 3:144873/149‑1 (MQ=255) | CGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTC‑‑AGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTA > NC_002947/2272923‑2273209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |