Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,284,012 A→G 5.4% Q71R (CAG→CGG)  ppnK → inorganic polyphosphate/ATP‑NAD kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,284,0120AG 5.4% 222.0 / 2.0 74Q71R (CAG→CGG) ppnKinorganic polyphosphate/ATP‑NAD kinase
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base G (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

AATTTCGCAATATCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTTCTCACC  >  NC_002947/2283865‑2284160
                                                                                                                                                   |                                                                                                                                                    
aaTTTCGCAATATCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAg                                                                                                                                                     <  1:194198/149‑1 (MQ=255)
  tttCGCAATATCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGAc                                                                                                                                                   >  2:222478/1‑149 (MQ=255)
      gCAATATCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCGGACGTc                                                                                                                                                >  3:178128/1‑148 (MQ=255)
      gCAATATCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCGGACGTc                                                                                                                                                >  3:184111/1‑148 (MQ=255)
      gCAATATCGCTCTCATCCGTCGCCCTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAACTTCCTCCTCGATCGCCACCTGCACGTGAGCCTCGAGGACACCATTGCCGCAGTGCTGCCCGGCCACGGCTTGCAGAc                                                                                                                                                   <  4:77510/145‑1 (MQ=255)
         atatCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCAcc                                                                                                                                            >  5:229169/1‑149 (MQ=255)
                           gCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGa                                                                                                                           <  5:319017/148‑1 (MQ=255)
                             cTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGt                                                                                                                        >  4:341168/1‑149 (MQ=255)
                              ttGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTc                                                                                                                       >  5:62756/1‑149 (MQ=255)
                                 gCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGc                                                                                                                    >  7:6526/1‑149 (MQ=255)
                                   aGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCGGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGa                                                                                                                  <  4:184111/149‑1 (MQ=255)
                                   aGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCGGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGa                                                                                                                  <  4:178128/149‑1 (MQ=255)
                                     cTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGAcc                                                                                                                <  4:140813/149‑1 (MQ=255)
                                                tCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTg                                                                                                     >  1:367148/1‑149 (MQ=255)
                                                  gACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCt                                                                                                               <  5:263967/137‑1 (MQ=255)
                                                  gACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCt                                                                                                               >  6:263967/1‑137 (MQ=255)
                                                  gACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGt                                                                                                   >  7:281919/1‑149 (MQ=255)
                                                  gACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGt                                                                                                   >  4:206332/1‑149 (MQ=255)
                                                   acacCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTc                                                                                                  <  6:162668/149‑1 (MQ=255)
                                                     acCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTcgg                                                                                                >  6:370084/1‑149 (MQ=255)
                                                       cATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGCcagcg                                                                                              >  4:259311/1‑149 (MQ=255)
                                                       cATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGACATCGTGGTcggcg                                                                                              >  5:5710/1‑149 (MQ=255)
                                                            gCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGAt                                                                                         <  2:205257/149‑1 (MQ=255)
                                                            gCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGAt                                                                                         >  4:284711/1‑149 (MQ=255)
                                                            gCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGAt                                                                                         >  2:321684/1‑149 (MQ=255)
                                                                  tGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGc                                                                                   >  1:190543/1‑149 (MQ=255)
                                                                  tGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGc                                                                                   >  8:46092/1‑149 (MQ=255)
                                                                     aaaaaTTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCctg                                                                                <  7:199786/149‑1 (MQ=255)
                                                                       aaaTTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCctgct                                                                              <  2:190543/149‑1 (MQ=255)
                                                                       aaaTTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCctgct                                                                              <  2:367148/149‑1 (MQ=255)
                                                                          ttCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCctgctgcg                                                                           >  1:137138/1‑147 (MQ=255)
                                                                                ctcGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGtgctgc                                                                     <  5:252021/149‑1 (MQ=255)
                                                                                ctcGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGtg                                                                         >  4:139373/1‑145 (MQ=255)
                                                                                ctcGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGtg                                                                         <  3:139373/145‑1 (MQ=255)
                                                                                ctcGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCCACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGtgctgc                                                                     >  7:81107/1‑149 (MQ=255)
                                                                                    aTCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCcgc                                                                 >  1:278683/1‑149 (MQ=255)
                                                                                       gCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCcgcgc                                                               <  3:341168/148‑1 (MQ=255)
                                                                                         cACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTTCTGGGTGCTGCCCGCGCGCt                                                            >  1:336766/1‑149 (MQ=255)
                                                                                               cACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGccc                                                       <  4:330932/148‑1 (MQ=255)
                                                                                               cACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGcc                                                        <  2:278683/147‑1 (MQ=255)
                                                                                                 cGTGATCCTCGATGACACCATTGCCGTAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGc                                                    <  5:184132/149‑1 (MQ=255)
                                                                                                      cgatCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCacaac                                               <  4:146176/146‑1 (MQ=255)
                                                                                                        cTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACAt                                             >  4:326227/1‑149 (MQ=255)
                                                                                                         tCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACAtt                                            >  7:103413/1‑149 (MQ=255)
                                                                                                         tCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCAACGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCTCGCGCGCTGGCCCGGCACAACAtt                                            >  4:246287/1‑149 (MQ=255)
                                                                                                           gAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTcc                                          >  7:86994/1‑149 (MQ=255)
                                                                                                             ggACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCgg                                        >  3:347504/1‑149 (MQ=255)
                                                                                                             ggACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCgg                                        >  8:30843/1‑149 (MQ=255)
                                                                                                             ggACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTCGTCATCGTGGTCGGCGGCGATGGCCGCCTGATGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCgg                                        >  2:53616/1‑149 (MQ=255)
                                                                                                                 acCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCt                                    >  3:255168/1‑149 (MQ=255)
                                                                                                                 acCATTGCCGAAGTGCTGCCCGGCCACGGCATGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCt                                    >  2:262777/1‑149 (MQ=255)
                                                                                                                   cATTGGCGAAGTGCTGCTCGGCCAGGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGACATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCTCTGGCCCGGCACAACATTCCGGTGCTgg                                  <  6:5710/149‑1 (MQ=255)
                                                                                                                   cATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCacaa                                                <  2:336104/135‑1 (MQ=255)
                                                                                                                   cATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCacaa                                                >  1:336104/1‑135 (MQ=255)
                                                                                                                   cATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGATCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTgg                                  >  5:221909/1‑149 (MQ=255)
                                                                                                                    aTTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTggg                                 <  6:62756/149‑1 (MQ=255)
                                                                                                                       gCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTa                               <  3:326227/148‑1 (MQ=255)
                                                                                                                           aaGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCcg                                                                  <  2:207720/109‑1 (MQ=255)
                                                                                                                           aaGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCcg                                                                  >  1:207720/1‑109 (MQ=255)
                                                                                                                              tgctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTTCGGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCTCAACATTCCGGTGCTGGGTATCAACCGt                       >  5:227364/1‑149 (MQ=255)
                                                                                                                              tgctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGt                       <  8:6526/149‑1 (MQ=255)
                                                                                                                              tgctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGt                       <  5:370084/149‑1 (MQ=255)
                                                                                                                               gctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTg                      <  2:137138/149‑1 (MQ=255)
                                                                                                                                ctgtCCGGCCACGGCTTGCAGACGTCCAGCCGCAAGCTGCGTGGCGAGGTCTGCGACGTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGGTGGCCCGGGACAACATTCCGGTGCTGGGTATCAACCGTg                      <  1:222522/148‑1 (MQ=255)
                                                                                                                                ctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTgg                     >  2:332424/1‑149 (MQ=255)
                                                                                                                                ctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTg                      <  1:222478/148‑1 (MQ=255)
                                                                                                                                ctgcCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTg                      <  7:30843/148‑1 (MQ=255)
                                                                                                                                     cGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCacaaca                                              <  4:366116/119‑1 (MQ=255)
                                                                                                                                        ccACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTgg             <  3:284711/149‑1 (MQ=255)
                                                                                                                                         cACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCACCCGTGGCAACCTggg            >  6:300259/1‑149 (MQ=255)
                                                                                                                                           cGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCAGCGACCTGGTCATCGTGGTCGGCGGCGAAGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTTTCAACCGTGGCAACCTGGGtt          >  5:220838/1‑149 (MQ=255)
                                                                                                                                              cTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCTCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTtc       <  3:246287/149‑1 (MQ=255)
                                                                                                                                                 gCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTTCTCa    >  8:332400/1‑149 (MQ=255)
                                                                                                                                                  cAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTTCTCAc   <  8:281919/149‑1 (MQ=255)
                                                                                                                                                   aGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTTCTCAcc  <  1:321684/149‑1 (MQ=255)
                                                                                                                                                   aGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTTCTCAcc  >  3:326093/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATTTCGCAATATCGGTATCATCGGTCGCCTTGGCAGCTCGCAGGTGCTCGACACCATTCGCCGACTGAAAAAATTCCTCCTCGATCGCCACCTGCACGTGATCCTCGAGGACACCATTGCCGAAGTGCTGCCCGGCCACGGCTTGCAGACGTCCACCCGCAAGCTGCTGGGCGAGGTCTGCGACCTGGTCATCGTGGTCGGCGGCGATGGCAGCCTGCTGGGTGCTGCCCGCGCGCTGGCCCGGCACAACATTCCGGTGCTGGGTATCAACCGTGGCAACCTGGGTTTTCTCACC  >  NC_002947/2283865‑2284160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑