Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,296,432	G→T	12.2%	L19M (CTG→ATG)	PP_2023 ←	glutathione S‑transferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,296,432	0	G	T	12.2%	137.5 / 8.0	49	L19M (CTG→ATG)	PP_2023	glutathione S‑transferase family protein
Reads supporting (aligned to +/- strand):  ref base G (18/25);  new base T (2/4);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TGATGTCCAGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGGATGCGCTCGTGCATTTCCTGTACGTGGGAGATCACC  >  NC_002947/2296285‑2296576                                                                                                                                                    |                                                                                                                                                    tgatgTCCTGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAAGGCCAgg                                                                                                                                                    >  4:110745/1‑149 (MQ=255)   atgTCCAGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGgcg                                                                                                                                                  >  4:68899/1‑149 (MQ=255)        cAGGCTCTCTCCCAGTACCTGGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGAGAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGcgca                                                                                                                                              <  4:282832/148‑1 (MQ=255)        cAGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGcgcac                                                                                                                                             >  6:377280/1‑149 (MQ=255)         aGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGcgcacg                                                                                                                                            >  2:70493/1‑149 (MQ=255)         aGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTCCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGcgcacg                                                                                                                                            >  8:252921/1‑149 (MQ=255)          ggCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTGCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGcgcacg                                                                                                                                            >  4:243735/1‑148 (MQ=255)           gCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCATGCGtgcgcgcc                                                                                                                                          >  6:46717/1‑148 (MQ=255)               tctcCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATg                                                                                                                                        <  4:364067/147‑1 (MQ=255)                 tcCCTGTACCTCGTCACCGGTGTCGAGAACGGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATGGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCATGCGTGCGCGCCTgg                                                                                                                                       <  5:301522/146‑1 (MQ=255)                 tcCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCATGCGTGCGCGCCTgg                                                                                                                                       <  5:46717/146‑1 (MQ=255)                 tcCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGc                                                                                                                                      <  5:377280/147‑1 (MQ=255)                 tcCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGG‑CAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCATGCGTGCGCGCCTGGcc                                                                                                                                     <  6:258365/147‑1 (MQ=255)                   ccAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCATGCGTGCGCGCCTGGcc                                                                                                                                     <  6:248815/146‑1 (MQ=255)                   ccAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCa                                                                                                                                   <  8:71244/148‑1 (MQ=255)                   ccAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCAc                                                                                                                                  <  7:44494/149‑1 (MQ=255)                   ccAGTACCACGGCACCGGTGTCCAGCTCCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCTCCACCACGCAGATTTACACCTCGCACCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGcgacc                                                                                                                                  >  7:259968/1‑145 (MQ=255)                      gTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACgg‑g                                                                                                                              <  8:38054/149‑1 (MQ=255)                          cTCGGCACCGGTGTGCTGCTCCGGTAGCGTGCGCTTGGGCGAGAAGGCCAGCTGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCTCCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAg‑c                                                                                                                         <  8:354739/149‑1 (MQ=255)                                 ccGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGaa                                                                                                                  <  6:86534/149‑1 (MQ=255)                                 ccGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGaa                                                                                                                  <  8:267309/149‑1 (MQ=255)                                    gtgtCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGa                                                                                                               <  3:68899/149‑1 (MQ=255)                                       tCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTa                                                                                                            <  2:26741/149‑1 (MQ=255)                                         cAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTACTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTaga                                                                                                          <  1:309281/149‑1 (MQ=255)                                           gCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTagag                                                                                                         >  3:71864/1‑148 (MQ=255)                                            cACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGa                                                                                                       <  3:243735/149‑1 (MQ=255)                                                     cGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAAGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGca                                                                                              <  3:110745/149‑1 (MQ=255)                                                         cccTTGGGCGACAAGGCCAGCAGCTCTCCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACAcc                                                                                          <  7:252921/149‑1 (MQ=255)                                                                     aaGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTg                                                                              <  1:70493/149‑1 (MQ=255)                                                                                ctctGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCATGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTAcc                                                                   >  4:311484/1‑149 (MQ=255)                                                                                ctctGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGACCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCCCACACCTCCACGTCACTGAGGCCATTAcc                                                                   >  6:330560/1‑149 (MQ=255)                                                                                  ctGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAAc                                                                                                                 >  5:93861/1‑101 (MQ=255)                                                                                  ctGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAAc                                                                                                                 <  6:93861/101‑1 (MQ=255)                                                                                  ctGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGAATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCt                                                                 >  4:299318/1‑149 (MQ=255)                                                                                      cGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTaga                                                                                                          <  8:66670/104‑1 (MQ=255)                                                                                      cGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTaga                                                                                                          >  7:66670/1‑104 (MQ=255)                                                                                      cGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGgcg                                                             >  3:54035/1‑149 (MQ=255)                                                                                          ttGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGACTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGcgccg                                                          >  2:83808/1‑148 (MQ=255)                                                                                                    tCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGGAAAAGCCGGCGGACCGAGTGGAGGATCACCCCCACCCCTCCACGGAACTGAGGCCATTACCCTGGCCCCGC‑ACCTGGa                                                 >  1:119116/1‑148 (MQ=255)                                                                                                          ccTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCATGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCg                                        <  3:311484/149‑1 (MQ=255)                                                                                                          ccTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTg                                                   <  6:14982/138‑1 (MQ=255)                                                                                                          ccTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CCGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTg                                                   >  5:14982/1‑138 (MQ=255)                                                                                                                        aCCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGGATGCGCTCGTGc                          <  5:181296/149‑1 (MQ=255)                                                                                                                             gCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGTATATTACATTGGCGGAGC‑ACCTTGATCTTCACCGGGATGCGCTCGTGCATTTc                     <  2:119116/149‑1 (MQ=255)                                                                                                                              caccccgCATCGCGCAGGCGCAGGCGCGCACGCATTGCCCACGG‑GCTG‑CGGCGGATCGACTAGATGATCACCCGCACACCTCCACGTAAATTTTTTTTTTTCATTGGGGGAGC‑ACCTTGATCTTCACCGGGATGCGCTCGTGCATTTcc                    <  8:259968/143‑1 (MQ=255)                                                                                                                                gCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGTATGCGCTCGTGCATTTCCTg                  <  5:330560/149‑1 (MQ=255)                                                                                                                                    gCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGGATGCGCTCGTGCATTTCCTGTACg              >  4:292365/1‑149 (MQ=255)                                                                                                                                          ggcAGGGCCAGGCGCGGACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTGAATGAGGCCATTACCCTGGGGCCGCTACCTGGATCTGCACCGGGATGCGCTCGTGCATTTCCTGTACGTGGga         <  1:83808/148‑1 (MQ=255)                                                                                                                                             tgggCCATGCGTGCGCGCCTGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCCTTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGGATGCGCTCGTGCATTTCCTGTACGTGGGAGAt      >  7:154349/2‑148 (MQ=255)                                                                                                                                                gCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGGATGCGCTCGTGCATTTACTGTACGTGCTAGATCaac  >  8:121957/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGATGTCCAGGCTCTCTCCCAGTACCTCGGCACCGGTGTCCAGCACCGGTACCGTGCCCTTGGGCGACAAGGCCAGCAGCTCTGCCGGCTTGTTCTTCATCGCCACCTCGCAGATTTCCACCTCGCAGCCGGCATAGCGCAGGGCCAGGCGCGCACGCATGGCCCACGG‑GCAG‑CGGCGGAACGAGTAGAGGATCACCCGCACACCTCCACGTCACTGAGGCCATTACCCTGGCGCCGC‑ACCTGGATCTGCACCGGGATGCGCTCGTGCATTTCCTGTACGTGGGAGATCACC  >  NC_002947/2296285‑2296576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑