Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,300,171 C→G 7.0% A396P (GCC→CCC)  sbcD ← exonuclease subunit SbcD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,300,1710CG 7.0% 173.0 / 4.0 57A396P (GCC→CCC) sbcDexonuclease subunit SbcD
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base G (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GAACAGGCCAGCGCTGGCCAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAA  >  NC_002947/2300023‑2300317
                                                                                                                                                    |                                                                                                                                                  
gAACAGGCCAGCGCTGGCCAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGc                                                                                                                                                    <  6:320237/149‑1 (MQ=255)
   cAGGCCATCGCTGGCCAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAg                                                                                                                                                 <  8:153324/149‑1 (MQ=255)
      gCCAGCGCTGGCCAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAgcgc                                                                                                                                              >  7:26903/1‑149 (MQ=255)
                gCCAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGAtcgtcg                                                                                                                                    >  3:336020/1‑149 (MQ=255)
                  cAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTcggc                                                                                                                                  >  5:279885/1‑149 (MQ=255)
                     gggTTCTGCGGTGAAATCGATGTCGATCGGGCCGGGCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCggg                                                                                                                               >  3:10465/1‑149 (MQ=255)
                       gatCTGCGGTGATATCGATGTCGATCGGGCCGGCCAGCGATGCGAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGt                                                                                                                             <  6:371228/147‑1 (MQ=255)
                       gTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGt                                                                                                                             <  8:60864/149‑1 (MQ=255)
                             cGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCg                                                                                                                        <  5:355351/148‑1 (MQ=255)
                             cGGGGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGc                                                                                                                          <  5:168865/146‑1 (MQ=255)
                              ggTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCg                                                                                                                        >  4:97403/1‑147 (MQ=255)
                                tGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAgg                                                                                                                    <  4:336020/149‑1 (MQ=255)
                                  aaaTCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGCGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCt                                                                                                                  >  1:145855/1‑149 (MQ=255)
                                    aTCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTg                                                                                                                >  8:112056/1‑149 (MQ=255)
                                       gATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGtt                                                                                                              <  6:356189/148‑1 (MQ=255)
                                        aTGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGAGCGTCGGCGGGGTTGCCGTAGGCTTGTTcc                                                                                                            >  8:206439/1‑149 (MQ=255)
                                        aTGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCCGCCAGCGCCTGATCGGCGGCGGGGTTGCCGTAGGCTTGTTcc                                                                                                            >  2:77634/1‑149 (MQ=255)
                                           tCGATCGGGCCGGCCGGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCa                                                                                                          <  3:136609/148‑1 (MQ=255)
                                                  ggccggccAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGc                                                                                                  <  5:262958/149‑1 (MQ=255)
                                                   gccggccAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGGCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAgg                                                                                                        <  8:220499/142‑1 (MQ=255)
                                                   gccggccAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGGCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAgg                                                                                                        >  7:214853/1‑142 (MQ=255)
                                                   gccggccAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGGCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAgg                                                                                                        >  7:220499/1‑142 (MQ=255)
                                                   gccggccAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGGCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAgg                                                                                                        <  8:214853/142‑1 (MQ=255)
                                                         cAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAg                                                                                           >  6:165098/1‑149 (MQ=255)
                                                              aTGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCtt                                                                                      >  7:306259/1‑149 (MQ=255)
                                                                gCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGGAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGc                                                                                    >  4:149506/1‑149 (MQ=255)
                                                                gCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGc                                                                                    >  7:310110/1‑149 (MQ=255)
                                                                     gTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACa                                                                                            >  2:329329/1‑136 (MQ=255)
                                                                     gTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACa                                                                                            <  1:329329/136‑1 (MQ=255)
                                                                            aGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTg                                                                         <  3:97403/148‑1 (MQ=255)
                                                                                    ggCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTc                                                                >  6:47562/1‑149 (MQ=255)
                                                                                          aaTCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACTGCAGGGCGAAGTCGGCCAGCGCCGGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAAGAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACaaa                                                          <  2:30076/149‑1 (MQ=255)
                                                                                          aaTCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACaaa                                                          <  2:56943/149‑1 (MQ=255)
                                                                                           aTCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAg                                                         <  7:112056/149‑1 (MQ=255)
                                                                                            tCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAgg                                                        >  1:363804/1‑149 (MQ=255)
                                                                                                cATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGcc                                                      <  5:188527/147‑1 (MQ=255)
                                                                                                 cgtgCTGTTACTCTTCCTGCTGCCCGTCCTGCACCAGCAGGGCGAAGTCGGCCAGCTCGTGATCGTCGGCGGGGTGGCCGTAGGCTTGTGCGCAGGCGCGGCGGATCAGGGCTTGCGGGGTCATCTGGGCCAGTTCGACACAGGCCAgg                                                   <  4:291736/146‑1 (MQ=255)
                                                                                                 aTGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAgg                                                   <  8:306259/149‑1 (MQ=255)
                                                                                                   ggCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAg                                                    >  8:227166/1‑146 (MQ=255)
                                                                                                   ggCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAg                                                    <  7:227166/146‑1 (MQ=255)
                                                                                                            tctTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTc                                        >  1:263595/1‑149 (MQ=255)
                                                                                                              ttCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCgg                                      >  2:133206/1‑149 (MQ=255)
                                                                                                                ccTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCg                                       <  8:310110/146‑1 (MQ=255)
                                                                                                                   gctgcACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAg                                                                                                         <  8:197381/77‑1 (MQ=255)
                                                                                                                   gctgcACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAg                                                                                                         >  7:197381/1‑77 (MQ=255)
                                                                                                                   gctgcACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCg                                    >  8:322808/1‑146 (MQ=255)
                                                                                                                   gctgcACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGGCATCGTCTTCGGCg                                    <  7:322808/146‑1 (MQ=255)
                                                                                                                    ctgcACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCgttgt                                <  1:77634/149‑1 (MQ=255)
                                                                                                                     tgcACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCgttgtt                               >  1:124519/1‑149 (MQ=255)
                                                                                                                       cACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGttgttg                              <  6:279885/148‑1 (MQ=255)
                                                                                                                          gTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGcggc                          >  5:84910/1‑149 (MQ=255)
                                                                                                                           tCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGcggcc                         >  3:251827/1‑149 (MQ=255)
                                                                                                                             cTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCTAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGcggccg                        <  4:10465/148‑1 (MQ=255)
                                                                                                                                     gcagGGCGAAGTCGGCCAGCGCCTGCTCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCAGGCGTACTCg               >  3:224487/1‑149 (MQ=255)
                                                                                                                                          gCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCt          <  2:363804/149‑1 (MQ=255)
                                                                                                                                              aggcGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGGCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATa      <  5:367818/146‑1 (MQ=255)
                                                                                                                                               gTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATAc     <  4:251827/149‑1 (MQ=255)
                                                                                                                                                   gCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTCCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGaa  >  2:136916/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAACAGGCCAGCGCTGGCCAAGGGTTCTGCGGTGAAATCGATGTCGATCGGGCCGGCCAGCGATGCCAGGTTCTTCAGGCGGATGGCGAGAATCTTCATGGCTGTTCCTCTTCCTGCTGCACGTCCTGCAACAGCAGGGCGAAGTCGGCCAGCGCCTGATCGTCGGCGGGGTTGCCGTAGGCTTGTTCCCAGGCGCGGCTGAACAGGTCTTGCGGGGTCATCTGGGCCAGTTCGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAA  >  NC_002947/2300023‑2300317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑