Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,311,049	T→C	7.6%	V267A (GTA→GCA) ‡	PP_2033 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,311,049	0	T	C	7.6%	187.5 / 4.8	66	V267A (GTA→GCA) ‡	PP_2033	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base C (3/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

AGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310902‑2311197                                                                                                                                                    |                                                                                                                                                      aGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTa                                                                                                                                                      <  6:6279/149‑1 (MQ=255)  ggACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAg                                                                                                                                                     <  5:159096/149‑1 (MQ=255)  ggACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAg                                                                                                                                                     <  7:136554/149‑1 (MQ=255)  ggACGAGCGGCGGCATAACTTCCAGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAg                                                                                                                                                     <  3:232802/149‑1 (MQ=255)      gAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAc                                                                                                                                                 >  5:113179/1‑149 (MQ=255)           gcggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGNAGTCCCAGTGCGTGCCTACAGGCAcgcact                                                                                                                                            <  1:35591/149‑4 (MQ=255)                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAg                                                                                                                                      <  2:289001/149‑1 (MQ=255)                   aCTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCa                                                                                                                                    <  4:244269/148‑1 (MQ=255)                    cTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGt                                                                                                                                  <  1:117657/149‑1 (MQ=255)                    cTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGt                                                                                                                                  <  7:119291/149‑1 (MQ=255)                        ccGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGcc                                                                                                                               <  8:157983/148‑1 (MQ=255)                         cGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCcgc                                                                                                                             >  1:205806/1‑149 (MQ=255)                              gTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACa                                                                                                                        >  1:46416/1‑149 (MQ=255)                              gTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACa                                                                                                                        >  5:349414/1‑149 (MQ=255)                                 cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATg                                                                                                                      <  5:51117/148‑1 (MQ=255)                                    aGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAc                                                                                                                                                 >  2:365545/1‑119 (MQ=255)                                    aGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAc                                                                                                                                                 <  1:365545/119‑1 (MQ=255)                                         ccACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGa                                                                                                             <  8:236495/149‑1 (MQ=255)                                          cACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGCCCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAg                                                                                                            >  5:85071/1‑149 (MQ=255)                                          cACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAg                                                                                                            >  8:4373/1‑149 (MQ=255)                                          cACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCATGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAg                                                                                                            >  4:352254/1‑149 (MQ=255)                                             gACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGc                                                                                                         >  7:262897/1‑149 (MQ=255)                                                       tgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCt                                                                                               >  6:129471/1‑149 (MQ=255)                                                         ccGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCtt                                                                                              <  6:113179/148‑1 (MQ=255)                                                         ccGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCATGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTc                                                                                             <  3:352254/149‑1 (MQ=255)                                                             gCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCg                                                                                         <  6:349414/149‑1 (MQ=255)                                                                tGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                                      <  6:64432/149‑1 (MQ=255)                                                                tGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCCTCGGCGttt                                                                                      <  1:134421/149‑1 (MQ=255)                                                                  cACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCg                                                                                            <  6:78262/141‑1 (MQ=255)                                                                  cACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCg                                                                                            >  5:78262/1‑141 (MQ=255)                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  4:268058/1‑149 (MQ=255)                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  8:199567/1‑149 (MQ=255)                                                                             tGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                                          >  5:299752/1‑148 (MQ=255)                                                                               ggggTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGaa                                                                       >  8:69983/1‑149 (MQ=255)                                                                                     gtTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAgg                                                                 <  4:127599/149‑1 (MQ=255)                                                                                     gtTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAg                                                                  <  8:125492/148‑1 (MQ=255)                                                                                         cccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgc                                                             <  8:93412/149‑1 (MQ=255)                                                                                          ccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                            >  4:230535/1‑149 (MQ=255)                                                                                          ccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                            >  6:59627/1‑149 (MQ=255)                                                                                           cGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcgc                                                           <  4:258601/149‑1 (MQ=255)                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  5:294289/1‑149 (MQ=255)                                                                                                   cTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGCGCCTTGCTGGCTTCGGCGTTTGCCGATTTGGGc                                                                          <  1:281121/126‑1 (MQ=255)                                                                                                   cTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGAGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                                          >  2:281121/1‑126 (MQ=255)                                                                                                    ttCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGAcc                                                  >  4:206382/1‑149 (MQ=255)                                                                                                         gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTg                                                <  2:205806/146‑1 (MQ=255)                                                                                                           gCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggag                                            >  4:140914/1‑148 (MQ=255)                                                                                                              ggTGTTGCTGTGCAATCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                        <  6:85071/149‑1 (MQ=255)                                                                                                               gtgtTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGcc                                       >  3:165981/1‑149 (MQ=255)                                                                                                                    gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   <  5:59627/148‑1 (MQ=255)                                                                                                                    gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTg                                  >  1:37732/1‑149 (MQ=255)                                                                                                                     cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTCGCTACGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTCATGACCTGGGAGCAGCCGATTGc                                 >  5:79658/1‑149 (MQ=255)                                                                                                                        tgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                               <  6:294289/148‑1 (MQ=255)                                                                                                                            aGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTggg                          <  4:165981/149‑1 (MQ=255)                                                                                                                              ccGCAGTCCCAGTGCGTGCCTGTAGGCACGCGGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggc                         >  1:45458/1‑149 (MQ=255)                                                                                                                              ccGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcg                        >  6:63292/1‑149 (MQ=255)                                                                                                                                gCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc                      <  2:46416/149‑1 (MQ=255)                                                                                                                                gCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc                      >  2:242235/1‑149 (MQ=255)                                                                                                                                gCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc                      >  1:245651/1‑149 (MQ=255)                                                                                                                                  aGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGcggcgg                    >  3:121306/1‑149 (MQ=255)                                                                                                                                  aGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGcggcgg                    >  8:171572/1‑149 (MQ=255)                                                                                                                                      gacacgcgtgcCTACAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGGTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGCTTGCCGGTGGGCGGCGGGAc                 >  4:267842/6‑148 (MQ=255)                                                                                                                                      ccAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCgg                              >  6:171185/1‑135 (MQ=255)                                                                                                                                      ccAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCgg                              <  5:171185/135‑1 (MQ=255)                                                                                                                                          tgcgtgcCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCGGGTGGGGGGCGGGGCGAGgc            >  4:373181/1‑149 (MQ=255)                                                                                                                                           gcgtgcCTACAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATACGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgcg           >  3:246278/1‑149 (MQ=255)                                                                                                                                                 cTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAg     <  3:230535/149‑1 (MQ=255)                                                                                                                                                    tAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAg  <  7:69983/149‑1 (MQ=255)                                                                                                                                                    tAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAg  <  5:63292/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGC‑GTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310902‑2311197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑