Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,329,762 A→C 8.7% I39L (ATC→CTC)  PP_2048 → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,329,7620AC 8.7% 126.3 / 4.5 46I39L (ATC→CTC) PP_2048acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (22/20);  new base C (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGG  >  NC_002947/2329619‑2329907
                                                                                                                                                 |                                                                                                                                                 
cGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCtt                                                                                                                                              <  4:135162/149‑1 (MQ=255)
 gTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGcctcctc                                                                                                                                              >  3:57800/1‑147 (MQ=255)
  tttCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTga                                                                                                                                            <  2:113025/149‑1 (MQ=255)
       aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCACCTTCGCGAAATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGACAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCAGCCTTGaggagg                                                                                                                                       >  4:316082/1‑149 (MQ=255)
          cgcgTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCg                                                                                                                                    <  4:57800/149‑1 (MQ=255)
             gTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                 <  1:71107/149‑1 (MQ=255)
              tGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcga                                                                                                                                <  2:205901/149‑5 (MQ=255)
                gggAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGtt                                                                                                                              <  4:265126/149‑1 (MQ=255)
                 ggAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGttt                                                                                                                             <  3:178707/149‑1 (MQ=255)
                     gtacAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGAAACTCGCTAT‑‑CGAACTGGACAGCGACACACTCGACGGAATCCTTGAGGAGGCCGAACGATTCAc                                                                                                                          >  8:39876/4‑148 (MQ=255)
                     gTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCTTTTCTTGCTGCATGAACTGTTCGACCTGATCGTACATTGCCATCAACTCTTTAT‑‑CTAACTGGACCGCTACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCa                                                                                                                           <  7:39876/147‑1 (MQ=255)
                     gTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACt                                                                                                                         >  3:34343/1‑149 (MQ=255)
                      ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTg                                                                                                                        <  6:34321/149‑1 (MQ=255)
                      ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTg                                                                                                                        <  4:306092/149‑1 (MQ=255)
                             aTGAACTACCAGGCGCCCCTTCACGACATGCGTTTCGAGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTAGGTAT‑‑CGAACTGGAACGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACCTTTCACTGGc                                                                                                                      >  7:234920/1‑144 (MQ=255)
                                aaCTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGatcat                                                                                                              >  3:321/1‑149 (MQ=255)
                                  cTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCg                                                                                                            >  4:108654/1‑149 (MQ=255)
                                     ccAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAAGTCGGTAT‑‑CGAACTGGACCGCGACTCCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCAc                                                                                                         <  3:316082/149‑1 (MQ=255)
                                      cAGGCGCCCCTTCGCGTCATGCGTTTCGTGCTGCTTTAACTGTTCGACCTTTTCGGACATTGCCAGCATCTCGGTTT‑‑CGTTCTGGTCCGCGACATCTTCGACGGCATCCTTGAGGAGGTCGGATGTTTCTCTGGCCAGATCATCGCAc                                                                                                         <  8:234920/148‑1 (MQ=255)
                                                   gcgACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCaa                                                                                            <  4:331275/148‑1 (MQ=255)
                                                      aCATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATAGAGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACa                                                                                          >  1:302555/1‑149 (MQ=255)
                                                                 gTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAggg                                                                             <  3:108654/149‑1 (MQ=255)
                                                                  tgctgcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGc                                                                            >  5:169224/1‑149 (MQ=255)
                                                                        aTGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAg                                                                      <  5:151264/149‑1 (MQ=255)
                                                                           atcTGTTCGACCTGAGCGGACATTGCCAGCAACTCTGTAT‑‑CGAACTTGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCa                                                                                                                           <  4:64683/91‑1 (MQ=255)
                                                                           aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCa                                                                                                                           >  3:64683/1‑93 (MQ=255)
                                                                           aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                   <  6:82814/149‑1 (MQ=255)
                                                                                ttCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                     >  8:60428/1‑126 (MQ=255)
                                                                                ttCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                     <  7:60428/126‑1 (MQ=255)
                                                                                 tCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGc                                                             >  6:238528/1‑149 (MQ=255)
                                                                                               cATTGCAAGCAACTCGTTAT‑‑CGACCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCACCCTTGACGAACAGGGCTGTCTGTGGGTGGGCGGTGAGGTcaccac                                               <  4:34343/149‑1 (MQ=255)
                                                                                                aTTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                              >  7:345313/1‑149 (MQ=255)
                                                                                                aTTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                              >  7:350096/1‑149 (MQ=255)
                                                                                                     cAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGTGCGGTGAGGTCACCACCCCGGa                                         >  5:325802/1‑149 (MQ=255)
                                                                                                      aGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGAc                                        <  2:302555/149‑1 (MQ=255)
                                                                                                        cAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACgg                                      >  5:340169/1‑149 (MQ=255)
                                                                                                         aaCTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGc                                     <  3:82189/149‑1 (MQ=255)
                                                                                                                tAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCgg                               >  3:257554/1‑148 (MQ=255)
                                                                                                                       aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTACCCACCCCGGACGGCAGGCTGGACGcc                         >  8:265641/1‑149 (MQ=255)
                                                                                                                            gACCGCGACACCCTCGTCGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCTCATCATCGCTCCGCTCAATCGCAACTGTGTCGATCAGGGCTGTCAGTGGGTGGGCGGTGAGGTCAGCACCCGGGACGGCATACGGGACGCCTATc                     <  8:182780/148‑1 (MQ=255)
                                                                                                                            gACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCg                    >  7:32750/1‑149 (MQ=255)
                                                                                                                              ccGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCgg                   >  1:214484/1‑148 (MQ=255)
                                                                                                                                       ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTc          >  2:137800/1‑148 (MQ=255)
                                                                                                                                       ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGAAATActac          >  8:313437/1‑145 (MQ=255)
                                                                                                                                          gtgcGGCCTCCTCAAGGATGCCGCACGTTTCACTGGGCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGaa       >  6:224369/4‑148 (MQ=255)
                                                                                                                                          gtgcGGCCTCCTCAAGGATGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGaa       >  6:229505/4‑148 (MQ=255)
                                                                                                                                              ggCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGATGGCATGCGGGACGCCTATCGGCAATACGTCGAACATgg  >  7:102363/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGG  >  NC_002947/2329619‑2329907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑