Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,329,773 G→T 9.1% E42D (GAG→GAT)  PP_2048 → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,329,7730GT 9.1% 130.9 / 3.8 44E42D (GAG→GAT) PP_2048acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (22/18);  new base T (2/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

AACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCAT  >  NC_002947/2329626‑2329919
                                                                                                                                                     |                                                                                                                                                  
aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCACCTTCGCGAAATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGACAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCAGCCTTGaggagg                                                                                                                                                   >  4:316082/1‑149 (MQ=255)
   cgcgTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCg                                                                                                                                                <  4:57800/149‑1 (MQ=255)
      gTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                             <  1:71107/149‑1 (MQ=255)
       tGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcga                                                                                                                                            <  2:205901/149‑5 (MQ=255)
         gggAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGtt                                                                                                                                          <  4:265126/149‑1 (MQ=255)
          ggAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGttt                                                                                                                                         <  3:178707/149‑1 (MQ=255)
              gtacAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGAAACTCGCTAT‑‑CGAACTGGACAGCGACACACTCGACGGAATCCTTGAGGAGGCCGAACGATTCAc                                                                                                                                      >  8:39876/4‑148 (MQ=255)
              gTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCTTTTCTTGCTGCATGAACTGTTCGACCTGATCGTACATTGCCATCAACTCTTTAT‑‑CTAACTGGACCGCTACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCa                                                                                                                                       <  7:39876/147‑1 (MQ=255)
              gTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACt                                                                                                                                     >  3:34343/1‑149 (MQ=255)
               ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTg                                                                                                                                    <  4:306092/149‑1 (MQ=255)
               ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTg                                                                                                                                    <  6:34321/149‑1 (MQ=255)
                      aTGAACTACCAGGCGCCCCTTCACGACATGCGTTTCGAGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTAGGTAT‑‑CGAACTGGAACGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACCTTTCACTGGc                                                                                                                                  >  7:234920/1‑144 (MQ=255)
                         aaCTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGatcat                                                                                                                          >  3:321/1‑149 (MQ=255)
                           cTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCg                                                                                                                        >  4:108654/1‑149 (MQ=255)
                              ccAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAAGTCGGTAT‑‑CGAACTGGACCGCGACTCCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCAc                                                                                                                     <  3:316082/149‑1 (MQ=255)
                               cAGGCGCCCCTTCGCGTCATGCGTTTCGTGCTGCTTTAACTGTTCGACCTTTTCGGACATTGCCAGCATCTCGGTTT‑‑CGTTCTGGTCCGCGACATCTTCGACGGCATCCTTGAGGAGGTCGGATGTTTCTCTGGCCAGATCATCGCAc                                                                                                                     <  8:234920/148‑1 (MQ=255)
                                            gcgACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCaa                                                                                                        <  4:331275/148‑1 (MQ=255)
                                               aCATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATAGAGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACa                                                                                                      >  1:302555/1‑149 (MQ=255)
                                                          gTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAggg                                                                                         <  3:108654/149‑1 (MQ=255)
                                                           tgctgcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGc                                                                                        >  5:169224/1‑149 (MQ=255)
                                                                 aTGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAg                                                                                  <  5:151264/149‑1 (MQ=255)
                                                                    atcTGTTCGACCTGAGCGGACATTGCCAGCAACTCTGTAT‑‑CGAACTTGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCa                                                                                                                                       <  4:64683/91‑1 (MQ=255)
                                                                    aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCa                                                                                                                                       >  3:64683/1‑93 (MQ=255)
                                                                    aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                               <  6:82814/149‑1 (MQ=255)
                                                                         ttCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                                 >  8:60428/1‑126 (MQ=255)
                                                                         ttCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                                 <  7:60428/126‑1 (MQ=255)
                                                                          tCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGc                                                                         >  6:238528/1‑149 (MQ=255)
                                                                                        cATTGCAAGCAACTCGTTAT‑‑CGACCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCACCCTTGACGAACAGGGCTGTCTGTGGGTGGGCGGTGAGGTcaccac                                                           <  4:34343/149‑1 (MQ=255)
                                                                                         aTTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                                          >  7:350096/1‑149 (MQ=255)
                                                                                         aTTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                                          >  7:345313/1‑149 (MQ=255)
                                                                                              cAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGTGCGGTGAGGTCACCACCCCGGa                                                     >  5:325802/1‑149 (MQ=255)
                                                                                               aGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGAc                                                    <  2:302555/149‑1 (MQ=255)
                                                                                                 cAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACgg                                                  >  5:340169/1‑149 (MQ=255)
                                                                                                  aaCTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGc                                                 <  3:82189/149‑1 (MQ=255)
                                                                                                         tAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCgg                                           >  3:257554/1‑148 (MQ=255)
                                                                                                                aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTACCCACCCCGGACGGCAGGCTGGACGcc                                     >  8:265641/1‑149 (MQ=255)
                                                                                                                     gACCGCGACACCCTCGTCGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCTCATCATCGCTCCGCTCAATCGCAACTGTGTCGATCAGGGCTGTCAGTGGGTGGGCGGTGAGGTCAGCACCCGGGACGGCATACGGGACGCCTATc                                 <  8:182780/148‑1 (MQ=255)
                                                                                                                     gACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCg                                >  7:32750/1‑149 (MQ=255)
                                                                                                                       ccGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCgg                               >  1:214484/1‑148 (MQ=255)
                                                                                                                                ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTc                      >  2:137800/1‑148 (MQ=255)
                                                                                                                                ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGAAATActac                      >  8:313437/1‑145 (MQ=255)
                                                                                                                                   gtgcGGCCTCCTCAAGGATGCCGCACGTTTCACTGGGCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGaa                   >  6:224369/4‑148 (MQ=255)
                                                                                                                                   gtgcGGCCTCCTCAAGGATGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGaa                   >  6:229505/4‑148 (MQ=255)
                                                                                                                                       ggCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGATGGCATGCGGGACGCCTATCGGCAATACGTCGAACATgg              >  7:102363/1‑149 (MQ=255)
                                                                                                                                              ttGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGc        >  3:153828/1‑148 (MQ=255)
                                                                                                                                                gaggagGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGcc       <  4:257554/147‑1 (MQ=255)
                                                                                                                                                   gaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGTCGAACAGGGGTGTCAGTGGGTGGGCGGTGAGGTCCCCCCCCCGGACGGCTTGGGGGACGCCTTTGGGCAATAGGTGGAACATGGATGGGCAAGctt  >  1:289032/1‑147 (MQ=255)
                                                                                                                                                   gaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCAt  >  6:306376/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTAT‑‑CGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCAT  >  NC_002947/2329626‑2329919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑