Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,336,499 A→G 10.8% intergenic (‑43/+37) PP_2052 ← / ← PP_5500 bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,336,4990AG10.8% 92.5 / 2.6 37intergenic (‑43/+37)PP_2052/PP_5500bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/16);  new base G (2/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGGTGGGT  >  NC_002947/2336362‑2336646
                                                                                                                                         |                                                                                                                                                   
ttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAAc                                                                                                                                          >  8:156218/1‑149 (MQ=255)
ttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAAc                                                                                                                                          >  6:275386/1‑149 (MQ=255)
  gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGTAGATGGCGCTGAAAATCTGTTTACCGTGATAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACg                                                                                                                                         <  4:333132/148‑1 (MQ=255)
  gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACgg                                                                                                                                        >  5:201621/1‑149 (MQ=255)
  gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACgg                                                                                                                                        >  5:340470/1‑149 (MQ=255)
  gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACgg                                                                                                                                        >  6:95028/1‑149 (MQ=255)
   aGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgt                                                                                                                                       <  5:95028/149‑1 (MQ=255)
   aGTGTACAGAAGCGCAGGCGCTCGGTGTCGAAGATGGGGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagatgcc                                                                                                                                        <  3:40940/148‑5 (MQ=255)
    gtgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCCAAGATGGCTCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtg                                                                                                                                      <  1:240459/149‑1 (MQ=255)
     tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt                                                                                                                                     <  6:201621/149‑1 (MQ=255)
       tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCTCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt                                                                                                                                   <  8:214594/149‑1 (MQ=255)
         cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGa                                                                                                                                 <  6:340470/149‑1 (MQ=255)
         cTGAAGCGCAGGCGCTCGGTGGCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGa                                                                                                                                 <  8:63617/149‑1 (MQ=255)
               cgcAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCTCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCa                                                                                                                           <  2:130681/149‑1 (MQ=255)
               cgcAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCa                                                                                                                           <  7:156218/149‑1 (MQ=255)
                  aGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGATGCAGGCgagatgcc                                                                                                                                        <  2:4339/133‑5 (MQ=255)
                  aGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGATGCAGGCgagatgcc                                                                                                                                        >  1:4339/1‑129 (MQ=255)
                    gcgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTggg                                                                                                                      <  5:275386/149‑1 (MQ=255)
                        tCGGTGTCGAAGAGGGTGCCATGCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACCGGCGAGAACGGTGTGTGAGCTGCAGTggggg                                                                                                                    >  5:294875/1‑147 (MQ=255)
                        tCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTggggg                                                                                                                    >  3:156369/1‑147 (MQ=255)
                          ggTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCa                                                                                                                  <  5:293162/147‑1 (MQ=255)
                               cGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGt                                                                                                           >  1:196699/1‑149 (MQ=255)
                                    agGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCgggg                                                                                                      <  2:196699/149‑1 (MQ=255)
                                     gggTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCggggg                                                                                                     <  1:152154/149‑1 (MQ=255)
                                          ccatccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCacac                                                                                                >  2:19810/1‑149 (MQ=255)
                                                   tCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGATGCAGTGGGTGCAGTCAGCTCGGGGGCACAAGTTCGACAg                                                                                       >  7:327791/1‑149 (MQ=255)
                                                                         gTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCACGCCTCCAACAGGCGAGAACGGTGTGTGAGCTGCACTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACg                                                                   >  1:186246/1‑147 (MQ=255)
                                                                                          aGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGtt                                                                                             >  6:29663/1‑104 (MQ=255)
                                                                                          aGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGtt                                                                                             <  5:29663/104‑1 (MQ=255)
                                                                                            cATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAg                                              >  8:218929/1‑149 (MQ=255)
                                                                                                  cTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGc                                        >  2:342061/1‑149 (MQ=255)
                                                                                                                     cGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAgagga                              <  7:203418/140‑1 (MQ=255)
                                                                                                                     cGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAgagga                              >  8:203418/1‑140 (MQ=255)
                                                                                                                       tCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGATCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAAcc                   >  2:84973/1‑149 (MQ=255)
                                                                                                                             ttctcGCCTGTTGCAGTCGAGAACGGTGTGTGAGGTGCAGTGGGGGCAGTCAGGTGGGGGGCCCACGGTCGACAGGGGGACGCTCAGGAGTGCCGCAGAGGGTAGGCGGGGCGGAGCGGGGCGGGCAGAGGAGGTTGGCAACCGTTgcg             >  5:362066/2‑149 (MQ=255)
                                                                                                                                  gCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAgg                                                                          >  6:129020/1‑83 (MQ=255)
                                                                                                                                  gCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAgg                                                                          <  5:129020/83‑1 (MQ=255)
                                                                                                                                        aaCAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggtgggt  >  6:170091/1‑149 (MQ=255)
                                                                                                                                        aaCAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggtgggt  <  1:342061/149‑1 (MQ=255)
                                                                                                                                        aaCAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAgctgggt  >  4:358779/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
TTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGGTGGGT  >  NC_002947/2336362‑2336646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑