Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,342,178 T→A 6.7% T10T (ACT→ACA)  PP_2058 → porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,342,1780TA 6.7% 179.7 / 2.9 60T10T (ACT→ACA) PP_2058porin
Reads supporting (aligned to +/- strand):  ref base T (30/26);  new base A (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

TCTGGTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAGCGCCCGCGCCGCCGCCACTGC  >  NC_002947/2342031‑2342324
                                                                                                                                                   |                                                                                                                                                  
tctGGTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACtt                                                                                                                                                   >  1:352727/1‑149 (MQ=255)
tctGGTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACtt                                                                                                                                                   >  1:55487/1‑149 (MQ=255)
  tGGTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACtt                                                                                                                                                   <  2:359453/147‑1 (MQ=255)
  tGGTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTtgt                                                                                                                                                 >  7:25555/1‑149 (MQ=255)
   ggTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTtgt                                                                                                                                                 <  8:211422/148‑1 (MQ=255)
   ggTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTtgt                                                                                                                                                 >  7:211422/1‑148 (MQ=255)
    gTGATCACTGTGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTtgt                                                                                                                                                 <  5:181551/147‑1 (MQ=255)
    gTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTtgtg                                                                                                                                                <  5:135112/148‑1 (MQ=255)
        tCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTGCAAGTTTGGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAATAACAACAACATAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTTTGGCctg                                                                                                                                           <  4:59743/149‑1 (MQ=255)
          aCTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACAAGTGGccccac                                                                                                                                         <  2:365861/149‑5 (MQ=255)
              cgcTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACATAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGc                                                                                                                                     <  8:162724/149‑1 (MQ=255)
                     aCTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCggctggc                                                                                                                              >  4:296087/1‑149 (MQ=255)
                         ctgACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGc                                                                                                                           <  6:370313/148‑1 (MQ=255)
                            aCGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACCACAAGAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCt                                                                                                                         <  7:155459/147‑1 (MQ=255)
                              gggCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTgg                                                                                                                     <  7:220750/149‑1 (MQ=255)
                                gcgcCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGcc                                                                                                                   >  6:377955/1‑149 (MQ=255)
                                    cTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCggctgg                                                                                                                               >  4:74587/1‑133 (MQ=255)
                                    cTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCggctgg                                                                                                                               <  3:74587/133‑1 (MQ=255)
                                                  tttGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATc                                                                                                 <  2:352727/149‑1 (MQ=255)
                                                   ttGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATc                                                                                                 >  1:226162/1‑148 (MQ=255)
                                                      aaGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCg                                                                                                >  2:94568/1‑146 (MQ=255)
                                                          aCCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGa                                                                                            >  2:43003/1‑146 (MQ=255)
                                                          aCCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACa                                                                                          <  1:43003/148‑1 (MQ=255)
                                                          aCCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAg                                                                                         <  5:377955/149‑1 (MQ=255)
                                                                ggCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGcaagct                                                                                   >  2:131189/1‑149 (MQ=255)
                                                                   gccgcTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGcaagctc                                                                                  >  7:339036/1‑147 (MQ=255)
                                                                    ccgcTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGcaagctcaag                                                                               <  6:80434/149‑1 (MQ=255)
                                                                      gcTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATATCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCCGTGCCGCCGGTTTCCTCGACGACAGcaagatcacgct                                                                             >  8:275709/1‑149 (MQ=255)
                                                                       cTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTg                                                                            <  8:339036/149‑1 (MQ=255)
                                                                                      aCTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACgt                                                              <  8:25555/148‑1 (MQ=255)
                                                                                        tCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTa                                                           >  5:100820/1‑149 (MQ=255)
                                                                                        tCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTa                                                           >  2:159203/1‑149 (MQ=255)
                                                                                          aCCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAtt                                                         >  2:280726/1‑149 (MQ=255)
                                                                                          aCCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAtt                                                         >  6:56001/1‑149 (MQ=255)
                                                                                          aCCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAtt                                                         >  4:298136/1‑149 (MQ=255)
                                                                                          aCCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAtt                                                         >  4:160585/1‑149 (MQ=255)
                                                                                           cccAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAttt                                                        >  2:73581/1‑149 (MQ=255)
                                                                                            ccAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAttt                                                        >  3:40750/1‑148 (MQ=255)
                                                                                             cAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTc                                                       >  7:153148/1‑148 (MQ=255)
                                                                                             cAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTc                                                       >  1:157650/1‑148 (MQ=255)
                                                                                              aaGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAttt                                                        <  7:89967/146‑1 (MQ=255)
                                                                                              aaGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTAttt                                                        >  8:89967/1‑146 (MQ=255)
                                                                                                     actagagagTAACCATGTTGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCATGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGGAACGTGTATTTCAACGAGAAt                                              <  4:40750/144‑1 (MQ=255)
                                                                                                        acaGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTc                                           <  6:100820/149‑1 (MQ=255)
                                                                                                                  catcatGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGtt                                                                       >  3:324488/1‑111 (MQ=255)
                                                                                                                  catcatGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGtt                                                                       <  4:324488/111‑1 (MQ=255)
                                                                                                                  catcatGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAAc                                                                <  8:155880/118‑1 (MQ=255)
                                                                                                                  catcatGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAAc                                                                >  7:155880/1‑118 (MQ=255)
                                                                                                                  catcatGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAg                                  >  3:201760/1‑148 (MQ=255)
                                                                                                                   atcatGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAg                                  >  5:9372/1‑147 (MQ=255)
                                                                                                                      atGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAgg                              <  1:377105/148‑1 (MQ=255)
                                                                                                                       tGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGt                            <  3:160585/149‑1 (MQ=255)
                                                                                                                         tCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCAGCCTGTGGCCGCTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTCACGAGCAGGGTAt                          >  5:281981/1‑149 (MQ=255)
                                                                                                                           ccGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATc                                                                                                 <  7:166709/76‑1 (MQ=255)
                                                                                                                           ccGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATc                                                                                                 >  8:166709/1‑76 (MQ=255)
                                                                                                                            cGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAg                       >  4:159430/1‑149 (MQ=255)
                                                                                                                               tttCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAgcgc                    <  5:56001/149‑1 (MQ=255)
                                                                                                                                       ccGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAGCGCCCGcgcc             <  3:296087/148‑1 (MQ=255)
                                                                                                                                         agcaggCCACAAGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAGCGCCCGcgcagcc          >  4:154425/5‑149 (MQ=255)
                                                                                                                                          gcaggCCACAAGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGAc                                     <  5:242267/118‑1 (MQ=255)
                                                                                                                                          gcaggCCACAAGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGAc                                     >  6:242267/4‑121 (MQ=255)
                                                                                                                                                cACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAGCGCCCGCGCCGCCGCCACTg   <  1:159203/149‑1 (MQ=255)
                                                                                                                                                 aCTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAGCGCCCGCGCCGCCGCCACTGc  >  6:378498/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTGGTGATCACTGCGCTGGCACTGCTGACGGGCGCCTTGTCCTTCATGGTTTGAAGCACCCAGGGCGCCGCTCGGCGCCCCGCTGACTCACCCAAGAACAACAACAGAGTAACCATCATGTCCCGATTTCTTTTCCGTGGGGCCACTTGTGGCCTGCTGTGCGGCTGGCTGCCTGTGGCCGGTGCCGCCGGTTTCATCGAAGACAGCAAGCTCAAGCTGCAGTTGCGCAACGTGTATTTCAACGAGAATTTCCGTGACGAGCAGGGTATGAGCGCCCGCGCCGCCGCCACTGC  >  NC_002947/2342031‑2342324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑