Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,370,991 C→G 5.6% A1270G (GCC→GGC)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,370,9910CG 5.6% 228.4 / 4.3 71A1270G (GCC→GGC) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (27/40);  new base G (2/2);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

CCAGGCGCTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTG  >  NC_002947/2370845‑2371133
                                                                                                                                                   |                                                                                                                                              
ccAGGCGCTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGAAGGCCGACCTCGAGGCGCGGGGCAAGCTGGACAGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGcag                                                                                                                                              >  7:77105/1‑147 (MQ=255)
  aGGCGCTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGc                                                                                                                                            >  6:90177/1‑149 (MQ=255)
  aGGCGCTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGc                                                                                                                                            >  3:59536/1‑149 (MQ=255)
    gcgcTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGcc                                                                                                                                           <  1:239317/148‑1 (MQ=255)
    gcgcTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGcc                                                                                                                                           >  2:239317/1‑148 (MQ=255)
     cgcTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAg                                                                                                                                         <  1:190654/149‑1 (MQ=255)
      ggtttCGCTGGCCGCGCGCCGCGCCCGCGTGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAgg                                                                                                                                        <  7:54650/147‑1 (MQ=255)
      gcTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAgg                                                                                                                                        <  5:45052/149‑1 (MQ=255)
       cTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCCGGCAGCCAggc                                                                                                                                       <  4:20262/149‑2 (MQ=255)
        tttCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAAATAATACGCGACCGAAGAGCAGTTGGCCGACCGCATGGAGGCCGGCCAggg                                                                                                                                       >  1:128055/1‑149 (MQ=255)
          tCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGcc                                                                                                                                     <  5:148153/148‑1 (MQ=255)
          tCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGc                                                                                                                                      <  5:157945/147‑1 (MQ=255)
           cGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGcc                                                                                                                                           >  8:9942/1‑141 (MQ=255)
           cGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGcc                                                                                                                                           <  7:9942/141‑1 (MQ=255)
            gCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGa                                                                                                                                  <  5:85422/149‑1 (MQ=255)
              tGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGAcc                                                                                                                                >  7:268474/1‑149 (MQ=255)
                  cGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGTGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGCCCcgcg                                                                                                                            >  7:301993/1‑149 (MQ=255)
                    cTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGcc                                                                                                                          <  4:59536/149‑1 (MQ=255)
                       gccgcGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAg                                                                                                                       >  8:297824/1‑149 (MQ=255)
                       gccgcGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAg                                                                                                                       >  2:278950/1‑149 (MQ=255)
                         cgcgCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCt                                                                                                                     <  7:15032/149‑1 (MQ=255)
                         cgcgCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCt                                                                                                                     >  4:8025/1‑149 (MQ=255)
                               cgcgAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCCGGCAGCCAgg                                                                                                                                        >  3:344586/1‑124 (MQ=255)
                               cgcgAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCCGGCAGCCAgg                                                                                                                                        <  4:344586/124‑1 (MQ=255)
                                gcgAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTg                                                                                                              >  3:30962/1‑149 (MQ=255)
                                 cgAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGc                                                                                                             <  6:274133/149‑1 (MQ=255)
                                  gAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCGATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCt                                                                                                            <  8:262785/149‑1 (MQ=255)
                                       gATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGAtct                                                                                                       >  5:266276/1‑149 (MQ=255)
                                            ccGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGTGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTAc                                                                                                  <  7:342659/149‑1 (MQ=255)
                                            ccGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTa                                                                                                   <  3:179571/148‑1 (MQ=255)
                                             cGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTa                                                                                                   >  1:210445/1‑147 (MQ=255)
                                             cGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTa                                                                                                   >  5:240728/1‑147 (MQ=255)
                                                 tACAAGCGCCTGAAGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCaa                                                                                             <  8:77105/149‑1 (MQ=255)
                                                   cAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGa                                                                                           <  8:268474/149‑1 (MQ=255)
                                                    aaGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGAt                                                                                          >  3:5100/1‑149 (MQ=255)
                                                      gcgcCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATc                                                                                         >  4:361992/1‑148 (MQ=255)
                                                       cgcCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGa                                                                                       >  6:172041/1‑149 (MQ=255)
                                                        gcCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGAc                                                                                      <  4:30962/149‑1 (MQ=255)
                                                          cTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCt                                                                                    >  3:139975/1‑149 (MQ=255)
                                                            gATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTc                                                                                   >  7:310431/1‑148 (MQ=255)
                                                              tGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAg                                                                                <  1:200420/149‑1 (MQ=255)
                                                              tGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAg                                                                                <  2:34962/149‑1 (MQ=255)
                                                              tGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAg                                                                                <  1:278950/149‑1 (MQ=255)
                                                                  cGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGc                                                                            <  2:122209/149‑1 (MQ=255)
                                                                    aCCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCaa                                                                          <  2:128055/149‑1 (MQ=255)
                                                                         gAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGc                                                                      <  4:41059/148‑1 (MQ=255)
                                                                         gAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGc                                                                      <  6:266276/148‑1 (MQ=255)
                                                                              gcgGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGt                                                                <  6:240728/149‑1 (MQ=255)
                                                                                        cTGGTCCGTGCCATCGTATTGCTGG‑GGTGGGAAGAGGAGTTGGCCGATCGCCTGGCTGCCGGCCAGGGCCTGGCCCGCGCCGAGCTGTCGGTGCTGATCTCGTTGAGGAAGATCGACGTCAAGGAGCAACTGCTCTAGTCGCTGGTGc                                                       <  8:101164/148‑1 (MQ=255)
                                                                                         tGGACCGTGCCATCGAATTCCTGC‑TGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCATGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCg                                                     >  6:311539/1‑149 (MQ=255)
                                                                                            aCCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGgac                                                  <  6:45620/149‑1 (MQ=255)
                                                                                               gTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGgacgac                                               <  4:5100/149‑1 (MQ=255)
                                                                                                  ccATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGgacgacga                                             <  4:60222/148‑1 (MQ=255)
                                                                                                  ccATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGgacgacga                                             <  4:139975/148‑1 (MQ=255)
                                                                                                   cATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGgacgacgac                                            >  2:11302/1‑148 (MQ=255)
                                                                                                   cATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACt                                           <  7:297824/149‑1 (MQ=255)
                                                                                                     tCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTAc                                         <  5:172041/149‑1 (MQ=255)
                                                                                                             cTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGAccc                                  <  3:8025/148‑1 (MQ=255)
                                                                                                             cTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGAc                                    >  5:145636/1‑146 (MQ=255)
                                                                                                             cTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGAc                                    <  6:145636/146‑1 (MQ=255)
                                                                                                                      cGAAGAGCAGTTGGCCGTACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATg                          >  3:157431/1‑148 (MQ=255)
                                                                                                                             cAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGc                  <  3:351648/149‑1 (MQ=255)
                                                                                                                              aGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCt                 <  8:310431/149‑1 (MQ=255)
                                                                                                                                ttGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCttt               >  3:3970/1‑149 (MQ=255)
                                                                                                                                ttGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCttt               <  2:210445/149‑1 (MQ=255)
                                                                                                                                   gCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTccc            >  5:192534/1‑149 (MQ=255)
                                                                                                                                   gCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACAGCTTTccc            >  5:308974/1‑149 (MQ=255)
                                                                                                                                       aaCGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCccgccg        >  5:81935/1‑149 (MQ=255)
                                                                                                                                          cccTGGCCGGCAGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTcc     >  6:202644/2‑149 (MQ=255)
                                                                                                                                           ccTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTccc    <  6:192534/149‑1 (MQ=255)
                                                                                                                                           ccTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTccc    <  6:81935/149‑1 (MQ=255)
                                                                                                                                               gCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTg  <  8:23414/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CCAGGCGCTTTCGCTGGCCGCTCGCCGCGCCCGCGAGCGGATTGCCGAGTACAAGCGCCTGATGGCCGACCTCGAGGCGCGGGGCAAGCTGGACCGTGCCATCGAATTCCTGC‑CGTCCGAAGAGCAGTTGGCCGAACGCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTG  >  NC_002947/2370845‑2371133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑