Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,378,650 G→C 6.4% V69L (GTT→CTT) ‡ PP_2086 → CrfX family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,378,6500GC 6.4% 192.3 / 3.4 62V69L (GTT→CTT) ‡PP_2086CrfX family protein
Reads supporting (aligned to +/- strand):  ref base G (26/32);  new base C (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

GGACGACCGGGACGACGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCC  >  NC_002947/2378511‑2378797
                                                                                                                                           |                                                                                                                                                   
ggacgaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCAACATGCGCCga                                                                                                                                            <  3:131488/149‑2 (MQ=255)
 gacgaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGt                                                                                                                                           <  7:140246/149‑1 (MQ=255)
    gaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCg                                                                                                                                        >  1:202772/1‑149 (MQ=255)
      ccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTc                                                                                                                                      >  7:72252/1‑149 (MQ=255)
       cgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTc                                                                                                                                      >  1:149843/1‑148 (MQ=255)
        gggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAACCCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGa                                                                                                                                    <  2:250650/149‑1 (MQ=255)
        gggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTCAACAAGGGCTCGGCGCATGTTGCGCCGGTGCGTCGa                                                                                                                                    >  7:328363/1‑149 (MQ=255)
             gacgCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACActc                                                                                                                               >  2:277975/1‑149 (MQ=255)
                         cTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTccc                                                                                                                          >  1:200472/1‑142 (MQ=255)
                         cTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTccc                                                                                                                          <  2:200472/142‑1 (MQ=255)
                         cTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGc                                                                                                                   >  1:380992/1‑149 (MQ=255)
                         cTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGc                                                                                                                   >  3:140551/1‑149 (MQ=255)
                         cTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGATGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTccc                                                                                                                          >  1:206345/1‑142 (MQ=255)
                         atcggTGGCGTGCTGAAAACGGCCAACCGCCTGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTccc                                                                                                                          <  2:206345/139‑1 (MQ=255)
                           gggTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTgcgc                                                                                                                                               >  1:86276/1‑119 (MQ=255)
                           gggTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTgcgc                                                                                                                                               <  2:86276/119‑1 (MQ=255)
                                gcgTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATAGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAAt                                                                                                            <  8:80585/149‑1 (MQ=255)
                                   tGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgc                                                                                                         <  8:104278/149‑1 (MQ=255)
                                     cTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgcag                                                                                                       <  2:202772/149‑1 (MQ=255)
                                     cTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgcag                                                                                                       <  5:101214/149‑1 (MQ=255)
                                           accgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGc                                                                                                 <  7:72692/149‑1 (MQ=255)
                                               ccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTc                                                                                                                            <  2:366999/118‑1 (MQ=255)
                                               ccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTc                                                                                                                            >  1:366999/1‑118 (MQ=255)
                                               ccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGa                                                                                              >  5:229282/1‑148 (MQ=255)
                                                  accgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGAGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAg                                                                                          >  3:361219/1‑149 (MQ=255)
                                                     gccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGcc                                                                                       <  6:235409/149‑1 (MQ=255)
                                                         ggTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGAtt                                                                                   <  6:85311/149‑1 (MQ=255)
                                                          gTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGa                                                                                              >  3:20483/1‑137 (MQ=255)
                                                          gTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGa                                                                                              <  4:20483/137‑1 (MQ=255)
                                                          gTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGAtt                                                                                   <  4:140551/148‑1 (MQ=255)
                                                                 cgGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATgg                                                                           <  2:380992/149‑1 (MQ=255)
                                                                      taTCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGc                                                                                                                <  2:37896/106‑1 (MQ=255)
                                                                      gATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGc                                                                                                                >  1:37896/1‑107 (MQ=255)
                                                                           gTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtct                                                                 >  4:369409/1‑149 (MQ=255)
                                                                           gTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtct                                                                 >  5:17357/1‑149 (MQ=255)
                                                                           gTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtct                                                                 >  5:12628/1‑149 (MQ=255)
                                                                            ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtctc                                                                >  3:295298/1‑149 (MQ=255)
                                                                               aGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTg                                                              <  6:229282/148‑1 (MQ=255)
                                                                               aGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTg                                                              <  8:97229/148‑1 (MQ=255)
                                                                                    gCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGAcc                                                          >  6:315379/1‑147 (MQ=255)
                                                                                     cTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCag                                                       <  1:277975/149‑1 (MQ=255)
                                                                                     cTTGGCCGATGGAGCGATGCGATGCTGATTGCCGTGAACAATGGCTCGGCGGATGTGGCGACGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGAccc                                                         <  8:305687/147‑1 (MQ=255)
                                                                                           cGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTg                                                 >  6:224112/1‑149 (MQ=255)
                                                                                                gAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCAc                                            >  5:117366/1‑149 (MQ=255)
                                                                                                 aGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCAcc                                           <  4:49643/149‑1 (MQ=255)
                                                                                                  gCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTg                                                 <  7:233316/142‑1 (MQ=255)
                                                                                                  gCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTg                                                 >  8:233316/1‑142 (MQ=255)
                                                                                                          ctgctgATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACt                                  <  5:224112/149‑1 (MQ=255)
                                                                                                          ctgctgATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACt                                  >  8:3346/1‑149 (MQ=255)
                                                                                                              tgATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCTGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGccc                              <  5:184558/149‑1 (MQ=255)
                                                                                                                   gCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATTAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTg                          <  1:90722/148‑1 (MQ=255)
                                                                                                                       tGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCa                     <  8:72252/149‑1 (MQ=255)
                                                                                                                         aaCAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCaa                    <  3:369409/148‑1 (MQ=255)
                                                                                                                          aCAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAgg                  <  7:3346/149‑1 (MQ=255)
                                                                                                                          aCAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACCCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAgg                  >  1:237302/1‑149 (MQ=255)
                                                                                                                             aTGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAg               >  2:348344/1‑149 (MQ=255)
                                                                                                                                 cTCGGCGCATGTTGCGCCGGAGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGaa           <  4:361219/149‑1 (MQ=255)
                                                                                                                                 accGGCGCAACATGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATGGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGaa           >  5:125928/3‑149 (MQ=255)
                                                                                                                                  ccGGCGCAACATGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAg               >  2:138243/2‑144 (MQ=255)
                                                                                                                                  ccGGCGCAACATGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAg               <  1:138243/143‑1 (MQ=255)
                                                                                                                                       gcATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCAt     <  4:295298/149‑1 (MQ=255)
                                                                                                                                          tGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATccc  <  3:211255/149‑1 (MQ=255)
                                                                                                                                           gTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGACCTTCATccc  >  1:162896/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
GGACGACCGGGACGACGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCC  >  NC_002947/2378511‑2378797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑