Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,389,141 T→C 8.4% M1T (ATG→ACG) † rlmKL → 23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,389,1410TC 8.4% 233.4 / 9.0 83M1T (ATG→ACG) †rlmKL23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (40/36);  new base C (3/4);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAG  >  NC_002947/2388995‑2389283
                                                                                                                                                  |                                                                                                                                              
gTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGc                                                                                                                                              <  7:126426/149‑1 (MQ=255)
 tACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCg                                                                                                                                             >  7:138121/1‑149 (MQ=255)
   cTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGt                                                                                                                                            <  8:21577/148‑1 (MQ=255)
   cTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGt                                                                                                                                            <  4:240251/148‑1 (MQ=255)
   cTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                           >  2:47101/1‑149 (MQ=255)
      ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                           <  6:337489/146‑1 (MQ=255)
      ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                           >  5:337489/1‑146 (MQ=255)
      ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGcc                                                                                                                                        <  1:47101/149‑1 (MQ=255)
       cttatcatCTAAGCTTGACTTTCCACTTGGCCAGGCAAGGATCGGGCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGccc                                                                                                                                       <  4:276896/143‑1 (MQ=255)
        tgatgaTCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGcccc                                                                                                                                      >  6:4146/1‑149 (MQ=255)
          atgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgt                                                                                                                                    <  6:373298/149‑1 (MQ=255)
          atgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGACGCATCGcccc                                                                                                                                      <  5:99503/147‑1 (MQ=255)
             atCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                  <  7:23689/148‑1 (MQ=255)
             atCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                  <  7:272832/148‑1 (MQ=255)
              tCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagt                                                                                                                                >  1:61892/1‑149 (MQ=255)
                tAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagtc                                                                                                                               >  5:354538/1‑148 (MQ=255)
                tAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagtc                                                                                                                               >  8:86909/1‑148 (MQ=255)
                 aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagtca                                                                                                                              >  6:367871/1‑148 (MQ=255)
                 aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                   <  4:273367/143‑3 (MQ=255)
                 aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                   <  4:268478/143‑3 (MQ=255)
                 aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                   >  3:268478/1‑141 (MQ=255)
                 aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                   >  3:273367/1‑141 (MQ=255)
                  aGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                            <  4:264153/149‑1 (MQ=255)
                      gTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                            >  1:52808/1‑145 (MQ=255)
                      gTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                            <  2:52808/145‑1 (MQ=255)
                       tCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAg                                                                                                                         <  6:150368/147‑1 (MQ=255)
                       tCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGAAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGGCGCCCCGTCAGTCAAATAGcc                                                                                                                       >  6:359161/1‑149 (MQ=255)
                         cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGccc                                                                                                                      >  3:333849/1‑148 (MQ=255)
                         cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcgat                                                                                                                                 >  5:335426/1‑133 (MQ=255)
                         cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcgat                                                                                                                                 <  6:335426/137‑5 (MQ=255)
                          ttGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGccc                                                                                                                      >  3:262365/1‑147 (MQ=255)
                               aCTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGgcgc                                                                                                               >  4:91051/1‑149 (MQ=255)
                                    gCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                          >  4:112853/1‑149 (MQ=255)
                                    gCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                          >  2:291893/1‑149 (MQ=255)
                                    gCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                          <  4:333849/149‑1 (MQ=255)
                                        ggCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACt                                                                                                      <  2:61892/149‑1 (MQ=255)
                                         gCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACt                                                                                                      >  2:281085/1‑148 (MQ=255)
                                           aCGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCAt                                                                                                   >  3:34000/1‑149 (MQ=255)
                                           aCGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCAt                                                                                                   >  2:343129/1‑149 (MQ=255)
                                             ggATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGt                                                                                                 >  8:83438/1‑149 (MQ=255)
                                                       ggcggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATa                                                                                                                          <  6:99782/114‑1 (MQ=255)
                                                       ggcggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATa                                                                                                                          >  5:99782/1‑114 (MQ=255)
                                                          ggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCg                                                                                     >  6:225957/1‑148 (MQ=255)
                                                            cccGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAAc                                                                                  <  5:4146/149‑1 (MQ=255)
                                                                 cTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAc                                                                             <  1:281085/149‑1 (MQ=255)
                                                                  ttttCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAcc                                                                            >  1:152403/1‑149 (MQ=255)
                                                                       tGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCAcc                                                                       >  7:302003/1‑149 (MQ=255)
                                                                            caCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTcc                                                                  <  7:83438/149‑1 (MQ=255)
                                                                             aCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTccc                                                                 <  1:291893/149‑1 (MQ=255)
                                                                                       tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGaa                                                                                   <  2:182796/121‑1 (MQ=255)
                                                                                       tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGaa                                                                                   >  1:182796/1‑121 (MQ=255)
                                                                                       tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCaaa                                                              >  3:5351/1‑142 (MQ=255)
                                                                                       tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCaaa                                                              <  4:5351/142‑1 (MQ=255)
                                                                                          cAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGaaa                                                    <  3:112853/149‑1 (MQ=255)
                                                                                           aGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAg                                                   <  4:262365/149‑1 (MQ=255)
                                                                                            gAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGc                                                  <  5:367871/149‑1 (MQ=255)
                                                                                            gAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGc                                                  <  3:91051/149‑1 (MQ=255)
                                                                                             aaGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGcc                                                 >  8:23145/1‑149 (MQ=255)
                                                                                              aGGCCACCCGCACGCCTGCTGGAAATGTGTACGGGCAAACATCGCGTGGCGATGCGTCGACCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCt                                                <  5:359161/149‑1 (MQ=255)
                                                                                               ggCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTg                                               >  5:67568/1‑149 (MQ=255)
                                                                                                gCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGc                                              >  7:319993/1‑149 (MQ=255)
                                                                                                 ccACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCt                                             >  8:267613/1‑149 (MQ=255)
                                                                                                   aCCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCg                                           >  8:230702/1‑149 (MQ=255)
                                                                                                           gCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAagag                                   >  2:128336/1‑149 (MQ=255)
                                                                                                            ccTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAgg                                  >  7:154644/1‑149 (MQ=255)
                                                                                                                   gAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAg                             <  8:319993/147‑1 (MQ=255)
                                                                                                                    aaaGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGc                          >  6:374049/1‑149 (MQ=255)
                                                                                                                     aaGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGc                          <  1:128336/148‑1 (MQ=255)
                                                                                                                       ggTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTc                       <  7:86909/149‑1 (MQ=255)
                                                                                                                        gtgtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCg                      >  2:377343/1‑149 (MQ=255)
                                                                                                                          gtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGc                    >  6:163590/1‑149 (MQ=255)
                                                                                                                           tACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGcc                   <  4:65210/149‑1 (MQ=255)
                                                                                                                           tACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGc                    <  7:230702/148‑1 (MQ=255)
                                                                                                                           tACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGACGGACCGATTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCAAAGGGCCTCGGcc                   >  6:156512/1‑149 (MQ=255)
                                                                                                                             cGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCtt                 <  4:34000/149‑1 (MQ=255)
                                                                                                                               ggCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGACAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGa               >  2:35625/1‑149 (MQ=255)
                                                                                                                                gCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc              <  7:23145/149‑1 (MQ=255)
                                                                                                                                gCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc              >  8:169743/1‑149 (MQ=255)
                                                                                                                                  aaaCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc              >  5:38781/1‑147 (MQ=255)
                                                                                                                                   aaCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAg           <  5:374049/149‑1 (MQ=255)
                                                                                                                                   aaCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAg           <  5:163590/149‑1 (MQ=255)
                                                                                                                                     cATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTGATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGt         >  1:294243/1‑149 (MQ=255)
                                                                                                                                            gggCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAg  <  6:38781/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAG  >  NC_002947/2388995‑2389283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑