Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,389,144 G→A 8.5% R2H (CGT→CAT)  rlmKL → 23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,389,1440GA 8.5% 230.1 / 8.7 82R2H (CGT→CAT) rlmKL23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (39/36);  new base A (3/4);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

TACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGCACACCTC  >  NC_002947/2388996‑2389291
                                                                                                                                                    |                                                                                                                                                   
tACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCg                                                                                                                                                     >  7:138121/1‑149 (MQ=255)
  cTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGt                                                                                                                                                    <  8:21577/148‑1 (MQ=255)
  cTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGt                                                                                                                                                    <  4:240251/148‑1 (MQ=255)
  cTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                                   >  2:47101/1‑149 (MQ=255)
     ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                                   <  6:337489/146‑1 (MQ=255)
     ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                                   >  5:337489/1‑146 (MQ=255)
     ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGcc                                                                                                                                                <  1:47101/149‑1 (MQ=255)
      cttatcatCTAAGCTTGACTTTCCACTTGGCCAGGCAAGGATCGGGCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGccc                                                                                                                                               <  4:276896/143‑1 (MQ=255)
       tgatgaTCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGcccc                                                                                                                                              >  6:4146/1‑149 (MQ=255)
         atgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgt                                                                                                                                            <  6:373298/149‑1 (MQ=255)
         atgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGACGCATCGcccc                                                                                                                                              <  5:99503/147‑1 (MQ=255)
            atCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                          <  7:272832/148‑1 (MQ=255)
            atCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                          <  7:23689/148‑1 (MQ=255)
             tCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagt                                                                                                                                        >  1:61892/1‑149 (MQ=255)
               tAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagtc                                                                                                                                       >  8:86909/1‑148 (MQ=255)
               tAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagtc                                                                                                                                       >  5:354538/1‑148 (MQ=255)
                aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagtca                                                                                                                                      >  6:367871/1‑148 (MQ=255)
                aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                           <  4:273367/143‑3 (MQ=255)
                aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                           <  4:268478/143‑3 (MQ=255)
                aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                           >  3:268478/1‑141 (MQ=255)
                aaGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcg                                                                                                                                           >  3:273367/1‑141 (MQ=255)
                 aGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                                    <  4:264153/149‑1 (MQ=255)
                     gTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                                    <  2:52808/145‑1 (MQ=255)
                     gTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                                    >  1:52808/1‑145 (MQ=255)
                      tCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAg                                                                                                                                 <  6:150368/147‑1 (MQ=255)
                      tCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGAAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGGCGCCCCGTCAGTCAAATAGcc                                                                                                                               >  6:359161/1‑149 (MQ=255)
                        cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGccc                                                                                                                              >  3:333849/1‑148 (MQ=255)
                        cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcgat                                                                                                                                         <  6:335426/137‑5 (MQ=255)
                        cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCACGGGGCGACGCATCGCCCCgcgat                                                                                                                                         >  5:335426/1‑133 (MQ=255)
                         ttGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGccc                                                                                                                              >  3:262365/1‑147 (MQ=255)
                              aCTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGgcgc                                                                                                                       >  4:91051/1‑149 (MQ=255)
                                   gCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                                  >  4:112853/1‑149 (MQ=255)
                                   gCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                                  >  2:291893/1‑149 (MQ=255)
                                   gCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                                  <  4:333849/149‑1 (MQ=255)
                                       ggCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACt                                                                                                              <  2:61892/149‑1 (MQ=255)
                                        gCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACt                                                                                                              >  2:281085/1‑148 (MQ=255)
                                          aCGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCAt                                                                                                           >  2:343129/1‑149 (MQ=255)
                                          aCGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCAt                                                                                                           >  3:34000/1‑149 (MQ=255)
                                            ggATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGt                                                                                                         >  8:83438/1‑149 (MQ=255)
                                                      ggcggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATa                                                                                                                                  <  6:99782/114‑1 (MQ=255)
                                                      ggcggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATa                                                                                                                                  >  5:99782/1‑114 (MQ=255)
                                                         ggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCg                                                                                             >  6:225957/1‑148 (MQ=255)
                                                           cccGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAAc                                                                                          <  5:4146/149‑1 (MQ=255)
                                                                cTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAc                                                                                     <  1:281085/149‑1 (MQ=255)
                                                                 ttttCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAcc                                                                                    >  1:152403/1‑149 (MQ=255)
                                                                      tGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCAcc                                                                               >  7:302003/1‑149 (MQ=255)
                                                                           caCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTcc                                                                          <  7:83438/149‑1 (MQ=255)
                                                                            aCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTccc                                                                         <  1:291893/149‑1 (MQ=255)
                                                                                      tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGaa                                                                                           <  2:182796/121‑1 (MQ=255)
                                                                                      tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGaa                                                                                           >  1:182796/1‑121 (MQ=255)
                                                                                      tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCaaa                                                                      <  4:5351/142‑1 (MQ=255)
                                                                                      tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCaaa                                                                      >  3:5351/1‑142 (MQ=255)
                                                                                         cAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGaaa                                                            <  3:112853/149‑1 (MQ=255)
                                                                                          aGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAg                                                           <  4:262365/149‑1 (MQ=255)
                                                                                           gAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGc                                                          <  3:91051/149‑1 (MQ=255)
                                                                                           gAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGc                                                          <  5:367871/149‑1 (MQ=255)
                                                                                            aaGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGcc                                                         >  8:23145/1‑149 (MQ=255)
                                                                                             aGGCCACCCGCACGCCTGCTGGAAATGTGTACGGGCAAACATCGCGTGGCGATGCGTCGACCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCt                                                        <  5:359161/149‑1 (MQ=255)
                                                                                              ggCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTg                                                       >  5:67568/1‑149 (MQ=255)
                                                                                               gCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGc                                                      >  7:319993/1‑149 (MQ=255)
                                                                                                ccACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCt                                                     >  8:267613/1‑149 (MQ=255)
                                                                                                  aCCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCg                                                   >  8:230702/1‑149 (MQ=255)
                                                                                                          gCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAagag                                           >  2:128336/1‑149 (MQ=255)
                                                                                                           ccTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAgg                                          >  7:154644/1‑149 (MQ=255)
                                                                                                                  gAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAg                                     <  8:319993/147‑1 (MQ=255)
                                                                                                                   aaaGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGc                                  >  6:374049/1‑149 (MQ=255)
                                                                                                                    aaGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGc                                  <  1:128336/148‑1 (MQ=255)
                                                                                                                      ggTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTc                               <  7:86909/149‑1 (MQ=255)
                                                                                                                       gtgtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCg                              >  2:377343/1‑149 (MQ=255)
                                                                                                                         gtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGc                            >  6:163590/1‑149 (MQ=255)
                                                                                                                          tACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGcc                           <  4:65210/149‑1 (MQ=255)
                                                                                                                          tACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGc                            <  7:230702/148‑1 (MQ=255)
                                                                                                                          tACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGACGGACCGATTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCAAAGGGCCTCGGcc                           >  6:156512/1‑149 (MQ=255)
                                                                                                                            cGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCtt                         <  4:34000/149‑1 (MQ=255)
                                                                                                                              ggCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGACAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGa                       >  2:35625/1‑149 (MQ=255)
                                                                                                                               gCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc                      <  7:23145/149‑1 (MQ=255)
                                                                                                                               gCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc                      >  8:169743/1‑149 (MQ=255)
                                                                                                                                 aaaCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc                      >  5:38781/1‑147 (MQ=255)
                                                                                                                                  aaCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAg                   <  5:163590/149‑1 (MQ=255)
                                                                                                                                  aaCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAg                   <  5:374049/149‑1 (MQ=255)
                                                                                                                                    cATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTGATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGt                 >  1:294243/1‑149 (MQ=255)
                                                                                                                                           gggCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAg          <  6:38781/149‑1 (MQ=255)
                                                                                                                                                   cGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGCACACCTc  <  7:267613/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGCGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGCACACCTC  >  NC_002947/2388996‑2389291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑