Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,397,173 G→C 9.1% R828R (CGC→CGG)  PP_2100 ← two‑component system sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,397,1730GC 9.1% 200.1 / 9.4 66R828R (CGC→CGG) PP_2100two‑component system sensor protein
Reads supporting (aligned to +/- strand):  ref base G (33/27);  new base C (2/4);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

GATGCCGATGCCACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGA  >  NC_002947/2397026‑2397319
                                                                                                                                                   |                                                                                                                                                  
gatggcgatgccAGTGTGGCGCAGCTCCAGCTCCATTACGGCTTGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACATGACCGTGCTCGGTGAACTTGATGGCATTGGTCACCAGGTTGGAGAGCACCTGCTTGAAg                                                                                                                                                    <  1:275434/148‑1 (MQ=255)
 atgccgatgccACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAgcg                                                                                                                                                  >  4:42926/1‑149 (MQ=255)
       atgccACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCgg                                                                                                                                            <  3:42926/149‑1 (MQ=255)
         gccACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGt                                                                                                                                          >  7:22287/1‑149 (MQ=255)
           cACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTc                                                                                                                                         >  5:117850/1‑148 (MQ=255)
           cACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTc                                                                                                                                         >  7:207182/1‑148 (MQ=255)
            aCTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCaa                                                                                                                                       <  6:117850/149‑1 (MQ=255)
                tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGACCCGCTGCGCTTCAAGCAg                                                                                                                                   <  7:348299/149‑1 (MQ=255)
                tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGACCCGCTGCGCTTCAAGCAg                                                                                                                                   <  5:50168/149‑1 (MQ=255)
                tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAg                                                                                                                                   <  3:98486/149‑1 (MQ=255)
                tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAg                                                                                                                                   >  6:89599/1‑149 (MQ=255)
                gcgcgCACCTCCCGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGACCCGCTGCGCTTCAAGCAg                                                                                                                                   <  2:275399/148‑1 (MQ=255)
                       ccTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATgg                                                                                                                            <  7:282263/149‑1 (MQ=255)
                        ctccagctccagTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTAAAGCGCAGCGGGTCAAGCAGGACATgg                                                                                                                            >  5:383785/1‑148 (MQ=255)
                         tccagctccagTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATgg                                                                                                                            >  4:104483/1‑147 (MQ=255)
                                      cGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCggg                                                                                                             <  5:89599/149‑1 (MQ=255)
                                            gtgtGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATc                                                                                                       >  4:881/1‑149 (MQ=255)
                                                  tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTg                                                                                                                     <  6:150760/129‑1 (MQ=255)
                                                  tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTg                                                                                                                     >  5:150760/1‑129 (MQ=255)
                                                  tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCAGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGCCATGGCAGCGTGAGGCCGGGGAGATCTGccaa                                                                                                 >  6:259114/1‑146 (MQ=255)
                                                  tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCAGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCAcc                                                                                                 >  6:263545/1‑149 (MQ=255)
                                                    gTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTc                                                                                               <  2:175753/149‑1 (MQ=255)
                                                    gTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTc                                                                                               >  5:181741/1‑149 (MQ=255)
                                                        cGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAgg                                                                                           >  4:227875/1‑149 (MQ=255)
                                                                agcagcCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGcc                                                                                   >  7:102090/1‑149 (MQ=255)
                                                                   agcCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCttt                                                                                <  3:104483/149‑1 (MQ=255)
                                                                          cGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCcgc                                                                         <  1:194016/149‑1 (MQ=255)
                                                                           gCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCcgcg                                                                        >  5:176700/1‑149 (MQ=255)
                                                                           gCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCcgcg                                                                        >  3:257266/1‑149 (MQ=255)
                                                                             tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTg                                                                                                     >  7:100559/1‑118 (MQ=255)
                                                                             tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTg                                                                                                     <  8:100559/118‑1 (MQ=255)
                                                                             tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGcc                                                                      >  5:52105/1‑149 (MQ=255)
                                                                             tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGcc                                                                      >  5:57486/1‑149 (MQ=255)
                                                                                tGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCCTTCTGCCGCGCCTGa                                                                   <  3:881/149‑1 (MQ=255)
                                                                                 gcgcACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGa                                                                   >  8:94380/1‑148 (MQ=255)
                                                                                 gcgcACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGAc                                                                  >  5:106410/1‑149 (MQ=255)
                                                                                     aCCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGTGCGATCTGCACCTCCAGGGCCAGTCCTTTCTGCCGCGCCTGACCATc                                                              <  6:181741/149‑1 (MQ=255)
                                                                                     aCCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATc                                                              >  3:43407/1‑149 (MQ=255)
                                                                                      ccTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCg                                                             <  1:205452/149‑1 (MQ=255)
                                                                                            cGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATg                                                       >  7:296455/1‑149 (MQ=255)
                                                                                                cgcGGTGAACTTGCTGGCCTTGCTCCCCCGGGCGCACCGCCCCTGCCTGAACCGCCGCGGGTCCACCAGGACATGGCAGCGGCCGGCCGGGGCCACCTCCCCCTCCACGCCCAGGCCTCCCTGCCGCGCCTGACCATCGAAAATgcgc                                                    <  6:106410/146‑1 (MQ=255)
                                                                                                cTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATgcgc                                                    <  4:43407/148‑1 (MQ=255)
                                                                                                 tCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATgcgcgc                                                  >  6:78379/1‑149 (MQ=255)
                                                                                                        aCTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGa                                            <  6:57486/148‑1 (MQ=255)
                                                                                                        aCTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGa                                            <  6:52105/148‑1 (MQ=255)
                                                                                                        aCTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAt                                           <  8:22287/149‑1 (MQ=255)
                                                                                                         cTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAtt                                          <  8:207182/149‑1 (MQ=255)
                                                                                                         cTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAtt                                          <  3:227875/149‑1 (MQ=255)
                                                                                                          ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAAt                                                        <  3:256538/134‑1 (MQ=255)
                                                                                                          ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAAt                                                        >  4:256538/1‑134 (MQ=255)
                                                                                                          ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAt                                           >  8:212082/1‑147 (MQ=255)
                                                                                                          ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAt                                           <  7:212082/147‑1 (MQ=255)
                                                                                                                  aTTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAgcg                                 >  5:152939/1‑149 (MQ=255)
                                                                                                                   ttGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTCCCGCGCCTCACCATCGAAAATGCGCGACACCGATTCGACCAgcgc                                >  8:245475/1‑149 (MQ=255)
                                                                                                                    tGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGcgcc                               >  4:164261/1‑149 (MQ=255)
                                                                                                                       tCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCt                                                                                  <  5:330336/95‑1 (MQ=255)
                                                                                                                       tCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCt                                                                                  >  6:330336/1‑95 (MQ=255)
                                                                                                                       tCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGcgccgc                             >  6:154699/1‑148 (MQ=255)
                                                                                                                           cAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGt                        <  5:78379/149‑1 (MQ=255)
                                                                                                                                  gACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGac                    >  2:47079/1‑146 (MQ=255)
                                                                                                                                  gACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGac                    <  1:47079/146‑1 (MQ=255)
                                                                                                                                      gCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTcc             <  2:25568/149‑1 (MQ=255)
                                                                                                                                       gtccTGCTTGACCCGCTGCGCTTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGAGCTGCAGCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCg            >  5:260567/3‑149 (MQ=255)
                                                                                                                                         ccTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCggg          <  3:164261/149‑1 (MQ=255)
                                                                                                                                           tGCTTGACCCGCTGCGCTTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGc        >  4:352353/1‑149 (MQ=255)
                                                                                                                                            gCTTGAAGCGCAGCGGCTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACAGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGc        >  5:275864/1‑148 (MQ=255)
                                                                                                                                             cTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGCGCCa      <  7:245475/149‑1 (MQ=255)
                                                                                                                                                 aaGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGa  >  5:174371/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGCCGATGCCACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGA  >  NC_002947/2397026‑2397319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑