Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,397,181 G→C 7.1% P826A (CCG→GCG)  PP_2100 ← two‑component system sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,397,1810GC 7.1% 213.6 / 6.3 70P826A (CCG→GCG) PP_2100two‑component system sensor protein
Reads supporting (aligned to +/- strand):  ref base G (34/31);  new base C (2/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

ATGCCACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGACAA  >  NC_002947/2397033‑2397322
                                                                                                                                                    |                                                                                                                                             
atgccACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCgg                                                                                                                                               <  3:42926/149‑1 (MQ=255)
  gccACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGt                                                                                                                                             >  7:22287/1‑149 (MQ=255)
    cACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTc                                                                                                                                            >  5:117850/1‑148 (MQ=255)
    cACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTc                                                                                                                                            >  7:207182/1‑148 (MQ=255)
     aCTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCaa                                                                                                                                          <  6:117850/149‑1 (MQ=255)
         tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGACCCGCTGCGCTTCAAGCAg                                                                                                                                      <  5:50168/149‑1 (MQ=255)
         tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGACCCGCTGCGCTTCAAGCAg                                                                                                                                      <  7:348299/149‑1 (MQ=255)
         tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAg                                                                                                                                      >  6:89599/1‑149 (MQ=255)
         tCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAg                                                                                                                                      <  3:98486/149‑1 (MQ=255)
         gcgcgCACCTCCCGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGACCCGCTGCGCTTCAAGCAg                                                                                                                                      <  2:275399/148‑1 (MQ=255)
                ccTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATgg                                                                                                                               <  7:282263/149‑1 (MQ=255)
                 ctccagctccagTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTAAAGCGCAGCGGGTCAAGCAGGACATgg                                                                                                                               >  5:383785/1‑148 (MQ=255)
                  tccagctccagTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATgg                                                                                                                               >  4:104483/1‑147 (MQ=255)
                               cGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCggg                                                                                                                <  5:89599/149‑1 (MQ=255)
                                     gtgtGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATc                                                                                                          >  4:881/1‑149 (MQ=255)
                                           tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTg                                                                                                                        <  6:150760/129‑1 (MQ=255)
                                           tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTg                                                                                                                        >  5:150760/1‑129 (MQ=255)
                                           tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCAGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGCCATGGCAGCGTGAGGCCGGGGAGATCTGccaa                                                                                                    >  6:259114/1‑146 (MQ=255)
                                           tGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCAGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCAcc                                                                                                    >  6:263545/1‑149 (MQ=255)
                                             gTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTc                                                                                                  >  5:181741/1‑149 (MQ=255)
                                             gTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTc                                                                                                  <  2:175753/149‑1 (MQ=255)
                                                 cGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAgg                                                                                              >  4:227875/1‑149 (MQ=255)
                                                         agcagcCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGcc                                                                                      >  7:102090/1‑149 (MQ=255)
                                                            agcCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCttt                                                                                   <  3:104483/149‑1 (MQ=255)
                                                                   cGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCcgc                                                                            <  1:194016/149‑1 (MQ=255)
                                                                    gCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCcgcg                                                                           >  3:257266/1‑149 (MQ=255)
                                                                    gCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCcgcg                                                                           >  5:176700/1‑149 (MQ=255)
                                                                      tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTg                                                                                                        >  7:100559/1‑118 (MQ=255)
                                                                      tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTg                                                                                                        <  8:100559/118‑1 (MQ=255)
                                                                      tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGcc                                                                         >  5:57486/1‑149 (MQ=255)
                                                                      tGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGcc                                                                         >  5:52105/1‑149 (MQ=255)
                                                                         tGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCCTTCTGCCGCGCCTGa                                                                      <  3:881/149‑1 (MQ=255)
                                                                          gcgcACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGa                                                                      >  8:94380/1‑148 (MQ=255)
                                                                          gcgcACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGAc                                                                     >  5:106410/1‑149 (MQ=255)
                                                                              aCCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGTGCGATCTGCACCTCCAGGGCCAGTCCTTTCTGCCGCGCCTGACCATc                                                                 <  6:181741/149‑1 (MQ=255)
                                                                              aCCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATc                                                                 >  3:43407/1‑149 (MQ=255)
                                                                               ccTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCg                                                                <  1:205452/149‑1 (MQ=255)
                                                                                     cGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATg                                                          >  7:296455/1‑149 (MQ=255)
                                                                                         cgcGGTGAACTTGCTGGCCTTGCTCCCCCGGGCGCACCGCCCCTGCCTGAACCGCCGCGGGTCCACCAGGACATGGCAGCGGCCGGCCGGGGCCACCTCCCCCTCCACGCCCAGGCCTCCCTGCCGCGCCTGACCATCGAAAATgcgc                                                       <  6:106410/146‑1 (MQ=255)
                                                                                         cTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATgcgc                                                       <  4:43407/148‑1 (MQ=255)
                                                                                          tCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATgcgcgc                                                     >  6:78379/1‑149 (MQ=255)
                                                                                                 aCTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGa                                               <  6:57486/148‑1 (MQ=255)
                                                                                                 aCTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGa                                               <  6:52105/148‑1 (MQ=255)
                                                                                                 aCTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAt                                              <  8:22287/149‑1 (MQ=255)
                                                                                                  cTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAtt                                             <  3:227875/149‑1 (MQ=255)
                                                                                                  cTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAtt                                             <  8:207182/149‑1 (MQ=255)
                                                                                                   ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAAt                                                           >  4:256538/1‑134 (MQ=255)
                                                                                                   ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAAt                                                           <  3:256538/134‑1 (MQ=255)
                                                                                                   ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAt                                              >  8:212082/1‑147 (MQ=255)
                                                                                                   ttGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGAt                                              <  7:212082/147‑1 (MQ=255)
                                                                                                           aTTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAgcg                                    >  5:152939/1‑149 (MQ=255)
                                                                                                            ttGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTCCCGCGCCTCACCATCGAAAATGCGCGACACCGATTCGACCAgcgc                                   >  8:245475/1‑149 (MQ=255)
                                                                                                             tGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGcgcc                                  >  4:164261/1‑149 (MQ=255)
                                                                                                                tCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCt                                                                                     >  6:330336/1‑95 (MQ=255)
                                                                                                                tCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCt                                                                                     <  5:330336/95‑1 (MQ=255)
                                                                                                                tCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGcgccgc                                >  6:154699/1‑148 (MQ=255)
                                                                                                                    cAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGt                           <  5:78379/149‑1 (MQ=255)
                                                                                                                           gACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGac                       >  2:47079/1‑146 (MQ=255)
                                                                                                                           gACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGac                       <  1:47079/146‑1 (MQ=255)
                                                                                                                               gCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTcc                <  2:25568/149‑1 (MQ=255)
                                                                                                                                gtccTGCTTGACCCGCTGCGCTTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGAGCTGCAGCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCg               >  5:260567/3‑149 (MQ=255)
                                                                                                                                  ccTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCggg             <  3:164261/149‑1 (MQ=255)
                                                                                                                                    tGCTTGACCCGCTGCGCTTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGc           >  4:352353/1‑149 (MQ=255)
                                                                                                                                     gCTTGAAGCGCAGCGGCTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACAGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGc           >  5:275864/1‑148 (MQ=255)
                                                                                                                                      cTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGCGCCa         <  7:245475/149‑1 (MQ=255)
                                                                                                                                          aaGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGa     >  5:174371/1‑149 (MQ=255)
                                                                                                                                             cgcAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTTCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGACaa  <  5:259114/149‑1 (MQ=255)
                                                                                                                                             cgcAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGAc                                                                     >  4:226457/1‑82 (MQ=255)
                                                                                                                                             cgcAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGAc                                                                     <  3:226457/82‑1 (MQ=255)
                                                                                                                                             cgcAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGACaa  <  5:154699/149‑1 (MQ=255)
                                                                                                                                             cgcAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGACTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGACaa  <  5:263545/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ATGCCACTGTCGCGCACCTCCAGCTCCAGTACGGCTGGTGTGCTGGTTCCGTCATCCAGCAGCCTGACGCTGATGCGCACCTGACCGTGCTCGGTGAACTTGATGGCATTGCTCACCAGGTTGGACAGCACCTGCTTGAAGCGCAGCGGGTCAAGCAGGACATGGCAGCGTGCGGCCGGGGCGATCTGCACCTCCAGGGCCAGGCCTTTCTGCCGCGCCTGACCATCGAAAATGCGCGCCACCGATTCGACCAGCGCCGCCAGGTCGACCACCTCCGGGGCCAGGGACAA  >  NC_002947/2397033‑2397322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑