Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,401,849 T→C 9.3% K14E (AAA→GAA)  dgt ← deoxyguanosinetriphosphate triphosphohydrolase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,401,8490TC 9.3% 115.9 / 2.4 43K14E (AAA→GAA) dgtdeoxyguanosinetriphosphate triphosphohydrolase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (17/22);  new base C (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCGCGTATGGATATGGTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTCGTATCGATGGAACGGGCCTC  >  NC_002947/2401704‑2401997
                                                                                                                                                  |                                                                                                                                                    
aGGCGCGTATGGATATGGTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGcc                                                                                                                                                   >  2:267831/1‑149 (MQ=255)
     cgTATGGATATGGTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGcc                                                                                                                                              <  5:328815/149‑1 (MQ=255)
            atatGGTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTGCCGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTcgc                                                                                                                                        <  1:267831/149‑1 (MQ=255)
            atatGGTCGTTGCTCGACACCGGGTGCACCTGGGTCTTACGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTcgcg                                                                                                                                       <  5:286823/149‑1 (MQ=255)
                 gTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTCGCCCAGCCGTTCGCGGGt                                                                                                                                    >  8:167183/1‑147 (MQ=255)
                 gTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTCGCCCAGCCGTTCGCGGGt                                                                                                                                    >  8:162384/1‑147 (MQ=255)
                  tCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAg                                                                                                                                 <  5:246494/149‑1 (MQ=255)
                  tCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAg                                                                                                                                 >  2:235928/1‑149 (MQ=255)
                     ttGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGc                                                                                                                                >  3:257830/1‑147 (MQ=255)
                         tCGACACCGGGTGCACCTGGGTCATGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCgg                                                                                                                                      >  1:40096/1‑137 (MQ=255)
                         tCGACACCGGGTGCACCTGGGTCATGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCgg                                                                                                                                      <  2:40096/137‑1 (MQ=255)
                           gACACCGGGTGCACCTGGGTCTTGCGGCCGAGG‑TGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGc                                                                                                                       <  7:334203/149‑1 (MQ=255)
                            acacCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTCGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGc                                                                                                                       <  7:167183/149‑1 (MQ=255)
                            acacCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTCGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGc                                                                                                                       <  7:162384/149‑1 (MQ=255)
                                      cACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAg                                                                                                              <  1:235928/148‑1 (MQ=255)
                                       aCCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTcgc                                                                                                                                        <  6:326074/121‑1 (MQ=255)
                                       aCCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTcgc                                                                                                                                        >  5:326074/1‑121 (MQ=255)
                                                ttGCGGCGGAGGCGGCGGAAGGCACCGGAGAAAATGCTGCGGTCGTGGTCTTTGTGGATGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCGGTTCGCGGGTAAGCAAGGTGTGc                                                                                                                       <  5:282561/129‑1 (MQ=255)
                                                ttGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCAGAGTTCATCGTCGCTGTACAGGGCTTTTCCCAGCCGTTCGCGGGTAAGCAAGGTGTGc                                                                                                                       >  6:282561/1‑129 (MQ=255)
                                                  gCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGtctc                                                                                                    <  5:223371/146‑1 (MQ=255)
                                                   cGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTg                                                                                                 <  2:87144/148‑1 (MQ=255)
                                                   cGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGt                                                                                                >  6:339025/1‑149 (MQ=255)
                                                     gCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCg                                                                                              >  3:177417/1‑149 (MQ=255)
                                                      ccGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAg                                                                                                              <  5:356046/132‑1 (MQ=255)
                                                      ccGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAg                                                                                                              >  6:356046/1‑132 (MQ=255)
                                                         aGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCg                                                                                          <  2:3298/149‑1 (MQ=255)
                                                           gcggcgGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGaa                                                                                        <  2:224541/149‑1 (MQ=255)
                                                               cggAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGgca                                                                                    <  5:339025/149‑1 (MQ=255)
                                                                ggAAGGCAGCGGAGTAATTGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGACCGAGTTCATCGTCGCTGTAGATGTCTTTGCAGAGCAGTTCGGGGTTAAGGAAGTTGTGCCAGTACAAGTGGCGGTGTACTGTCTTTAGaattctg                                                                                   <  2:94184/149‑6 (MQ=255)
                                                                            agaAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTg                                                                       <  3:180445/149‑1 (MQ=255)
                                                                             gaAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGc                                                                      <  4:177417/149‑1 (MQ=255)
                                                                                                        gAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCgaagaa                                             >  7:220527/1‑147 (MQ=255)
                                                                                                        gAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCgaagaa                                             >  1:9892/1‑147 (MQ=255)
                                                                                                              gCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTG‑AGGAAGCGCATTGTGCTGCgaa                                                <  7:24989/137‑1 (MQ=255)
                                                                                                              gCTG‑CGCACGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTG‑AGGAAGCGCATTGTGCTGCgaa                                                >  8:24989/1‑137 (MQ=255)
                                                                                                                tG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGcc                                   >  6:341255/1‑149 (MQ=255)
                                                                                                                    gcCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCGAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTGTCCTGTCGTTCGATGTCAGCAGCATTCCCTGCAGGAAGCGCATTGTGCTGCGAAGATGAGGCCAGCCCtt                                <  6:95447/149‑1 (MQ=255)
                                                                                                                        gAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGAt                            <  4:257830/149‑1 (MQ=255)
                                                                                                                           ttCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCa                         >  4:318246/1‑149 (MQ=255)
                                                                                                                           ttCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTAGCGGGTAAGAAAGGAGTGCCAGTCCAAGGCGCGCTCTCCTGTCGTTCGAAGGCAGCAGCAAACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCa                         >  2:25592/1‑149 (MQ=255)
                                                                                                                              aTCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTc                      >  3:200736/1‑149 (MQ=255)
                                                                                                                                   cgcTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTCGTATc                 <  3:78308/149‑1 (MQ=255)
                                                                                                                                               cTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTCGTATCGATGGAACGGGc     >  6:307335/1‑149 (MQ=255)
                                                                                                                                                tttGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGcc                                   <  6:256337/118‑1 (MQ=255)
                                                                                                                                                tttGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGcc                                   >  5:256337/1‑118 (MQ=255)
                                                                                                                                                tttGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTCGTATCGATGGAACGGGcc    >  3:109448/1‑149 (MQ=255)
                                                                                                                                                  tGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTCGTATCTATGGAAAGGGCctc  >  2:294281/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGGCGCGTATGGATATGGTCGTTGCTCGACACCGGGTGCACCTGGGTCTTGCGGCCGAGGCGGCGGAAGGCACCGGAGAAAATGATGCGGTCGTGGTCTTTGTGGAAGGGGCTG‑CGCCCGAGTTCATCGGCGCTGTACAGGGCTTTGCCCAGCCGTTCGCGGGTAAGCAAGGTGTGCCAGTCCAAGGCGCGGTCTCCTGTCGTTCGAAGGCAGCAGCATACCCTGCAGGAAGCGCATTGTGCTGCGAAGAAGAGGCCAGCCCTTTGATGCATTCGTATCGATGGAACGGGCCTC  >  NC_002947/2401704‑2401997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑