Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,402,311	A→T	7.5%	L19Q (CTG→CAG)	PP_2103 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,402,311	0	A	T	7.5%	186.0 / 3.0	63	L19Q (CTG→CAG)	PP_2103	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base T (2/3);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCACGCCG  >  NC_002947/2402165‑2402459                                                                                                                                                    |                                                                                                                                                     cACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCagcagc                                                                                                                                                    <  1:145142/149‑1 (MQ=255)  aCCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAgcg                                                                                                                                                   >  4:12254/1‑149 (MQ=255)      aTGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCtt                                                                                                                                               >  8:199721/1‑149 (MQ=255)         ccccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            <  8:142971/149‑1 (MQ=255)          cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcgc                                                                                                                                           <  2:234554/149‑1 (MQ=255)          cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            >  4:179825/1‑148 (MQ=255)           ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            >  7:44709/1‑147 (MQ=255)           ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCa                                                                                                                                          <  3:53024/149‑1 (MQ=255)             acaGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCC‑TGCGCatg                                                                                                                                        <  1:182297/149‑3 (MQ=255)              caGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGTTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCC‑TGCGCatgg                                                                                                                                       <  5:82257/149‑4 (MQ=255)                   gcgcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgc                                                                                                                                  >  3:248767/1‑149 (MQ=255)                     gcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGCCGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                <  5:221739/149‑1 (MQ=255)                     gcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                >  5:158045/1‑149 (MQ=255)                          cTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAc                                                                                                                           <  4:220838/149‑1 (MQ=255)                             gATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcgcg                                                                                                                        >  4:119621/1‑149 (MQ=255)                                  ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgc                                                                                                                                  <  4:248368/134‑1 (MQ=255)                                  ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgc                                                                                                                                  >  3:248368/1‑134 (MQ=255)                                  ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                   >  6:186791/1‑149 (MQ=255)                                  ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                   >  3:206914/1‑149 (MQ=255)                                    aGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTgg                                                                                                                 <  4:248767/149‑1 (MQ=255)                                    aGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTgg                                                                                                                 <  3:119621/149‑1 (MQ=255)                                     ggCCTTCATGTCTGGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGc                                                                                                                <  1:132381/149‑1 (MQ=255)                                      gCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCa                                                                                                               <  4:47922/149‑1 (MQ=255)                                        cTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGt                                                                                                             >  3:47707/1‑149 (MQ=255)                                         ttCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGtt                                                                                                            >  7:175271/1‑149 (MQ=255)                                           cATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGtt                                                                                                            <  2:218036/147‑1 (MQ=255)                                             tGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTgg                                                                                                        <  2:77729/149‑1 (MQ=255)                                                 gCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAgg                                                                                                    <  3:181223/149‑1 (MQ=255)                                                       tGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATg                                                                                              >  6:276826/1‑149 (MQ=255)                                                        gCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATgg                                                                                             >  1:120325/1‑149 (MQ=255)                                                         cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATgg                                                                                             >  2:315133/1‑148 (MQ=255)                                                         cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATgg                                                                                             <  8:175271/148‑1 (MQ=255)                                                            aTACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGACaa                                                                                          >  1:41779/1‑148 (MQ=255)                                                              aCCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgc                                                                                                                                             >  5:322279/1‑95 (MQ=255)                                                              aCCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgc                                                                                                                                             <  6:322279/95‑1 (MQ=255)                                                                cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgc                                                                                     >  7:200695/1‑149 (MQ=255)                                                                cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAAGCTCATGGCAACAGgc                                                                                     >  5:360047/1‑149 (MQ=255)                                                                     aCGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTc                                                                                 <  2:140619/148‑1 (MQ=255)                                                                     aCGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTc                                                                                 >  1:140619/1‑148 (MQ=255)                                                                              aGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTg                                                                       <  4:47707/149‑1 (MQ=255)                                                                                cGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGc                                                                     <  3:12254/149‑1 (MQ=255)                                                                                  gTTCAAGCAACTGACCGGACTCATGACGGATTTCCTTGCGGTGCATATACATGCGC‑AGGCGCAGCATCGCCTAGCGAAGTTCCCGCGGGTTAAGCGTGTTTGGCAGTTCTTTCAGGCTCATGGCAACAGGCGCTCCCTGACCTTAGCac                                                                   >  8:68349/1‑148 (MQ=255)                                                                                      aaGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTtc                                                               >  6:174083/1‑149 (MQ=255)                                                                                        gCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTtcgt                                                             <  4:206914/149‑1 (MQ=255)                                                                                                 cGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGa                                                          <  8:154783/143‑1 (MQ=255)                                                                                                 cGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGa                                                          >  7:154783/1‑143 (MQ=255)                                                                                                  ggACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGgc                                                   >  3:277824/1‑149 (MQ=255)                                                                                                         tGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCATTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGACGaat                                            >  7:175993/1‑148 (MQ=255)                                                                                                          gACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAgg                                           >  5:147461/1‑149 (MQ=255)                                                                                                          gACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAgg                                           >  5:335264/1‑149 (MQ=255)                                                                                                           aCGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAggg                                          <  1:315133/149‑1 (MQ=255)                                                                                                            cGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATAGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                         <  6:158045/149‑1 (MQ=255)                                                                                                             ggATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                         >  7:183713/1‑148 (MQ=255)                                                                                                             ggATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                         >  1:53770/1‑148 (MQ=255)                                                                                                                tttCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgat                                      >  2:39271/1‑148 (MQ=255)                                                                                                                    cTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGGTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtc                                 >  3:190336/1‑149 (MQ=255)                                                                                                                          gTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGaa                           <  2:120325/149‑1 (MQ=255)                                                                                                                           tGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGaa                           >  8:188895/1‑148 (MQ=255)                                                                                                                           tGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAac                          <  4:133967/149‑1 (MQ=255)                                                                                                                           tGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAac                          <  7:199721/149‑1 (MQ=255)                                                                                                                                     tGCGCAAGGCGCTGCTGCGCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCt                >  4:316955/1‑149 (MQ=255)                                                                                                                                        gcaaggcgctgCTGCGCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCtt               >  4:218563/4‑147 (MQ=255)                                                                                                                                        gc‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCtt               >  3:125791/1‑147 (MQ=255)                                                                                                                                           aggcgctgCTGCGCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGgc          <  3:218563/149‑1 (MQ=255)                                                                                                                                                  gcagcGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCAcgc    <  5:174083/149‑1 (MQ=255)                                                                                                                                                    agcGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCAcgccg  <  7:68349/149‑1 (MQ=255)                                                                                                                                                    agcGCCTTGCGCAGTTCGCGCGGGTTACGCGTGATTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCAcgcag  >  1:322867/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCACGCCG  >  NC_002947/2402165‑2402459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑