Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	81,989	C→T	8.3%	T100M (ACG→ATG)	PP_0071 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	81,989	0	C	T	8.3%	240.2 / 8.3	84	T100M (ACG→ATG)	PP_0071	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/42);  new base T (3/4);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-02

GCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTCCTACA  >  NC_002947/81841‑82135                                                                                                                                                     |                                                                                                                                                   gCCCTATCCCTGCGCAGCGCTTCCGCAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGAGCGAGGCGATACTCGATGGCATCGAGTCGAc                                                                                                                                                    <  5:347908/149‑1 (MQ=255) gCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGAc                                                                                                                                                    <  6:381581/149‑1 (MQ=255)  cccTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGAc                                                                                                                                                    <  7:268251/148‑1 (MQ=255)  cccTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACg                                                                                                                                                   <  7:295564/149‑1 (MQ=255)   ccTAAACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCTATGGCATCGAGTCGACGc                                                                                                                                                  >  4:20569/1‑149 (MQ=255)        cccTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACAACATGCGGTAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCATCg                                                                                                                                             <  8:209069/148‑1 (MQ=255)         ccTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGTGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCATCga                                                                                                                                            <  8:185108/149‑2 (MQ=255)         ccTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGAGTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCATCga                                                                                                                                            <  7:74471/149‑2 (MQ=255)         ccTGCGAATCGCTTACGAAACCGGCTACAAATCTTTTGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCTAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCTATACTCGATTGCGTCGACTCGATGCCATCga                                                                                                                                            <  1:374607/149‑2 (MQ=255)          cTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGcc                                                                                                                                           >  3:218561/1‑149 (MQ=255)          cTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGcc                                                                                                                                           >  1:27581/1‑149 (MQ=255)            gcgcAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGAGTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGt                                                                                                                                         >  6:22726/1‑149 (MQ=255)              gcAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCa                                                                                                                                       <  4:178442/149‑1 (MQ=255)               cAGCGCTTACGAAACCGGATACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCAc                                                                                                                                      >  1:341722/1‑149 (MQ=255)                   gcTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGcc                                                                                                                                   >  8:202344/1‑148 (MQ=255)                    cTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCa                                                                                                                                 <  8:253311/149‑1 (MQ=255)                     ttACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAg                                                                                                                                >  5:115145/1‑149 (MQ=255)                       aCGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGcc                                                                                                                              >  1:45556/1‑149 (MQ=255)                       aCGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGc                                                                                                                               >  2:375528/1‑148 (MQ=255)                         gAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTc                                                                                                                            >  4:2931/1‑149 (MQ=255)                            aCCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCaa                                                                                                                         <  7:202414/149‑1 (MQ=255)                            aCCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCaa                                                                                                                         <  5:42197/149‑1 (MQ=255)                                  tACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCa                                                                                                                     >  1:190277/1‑147 (MQ=255)                                    cAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgc                                                                                                                 <  2:94158/149‑1 (MQ=255)                                     aaaGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcg                                                                                                                <  6:128510/149‑1 (MQ=255)                                     aaaGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCACCAAGGCATAgcg                                                                                                                >  4:57195/1‑149 (MQ=255)                                      aaGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcgc                                                                                                               <  8:263624/149‑1 (MQ=255)                                       aGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCgct                                                                                                              >  6:220079/1‑149 (MQ=255)                                         cTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCgctg                                                                                                             <  1:375528/148‑1 (MQ=255)                                              gacgtaaTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGTTACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATc                                                                                                        <  6:259093/142‑1 (MQ=255)                                              gACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCTTGCTATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCGAATGCATAGCGCTGCTATc                                                                                                        <  3:214885/148‑1 (MQ=255)                                              gACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATc                                                                                                        <  3:182946/148‑1 (MQ=255)                                               aCTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGa                                                                                                      <  3:20569/149‑1 (MQ=255)                                               aCTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGa                                                                                                      <  3:6094/149‑1 (MQ=255)                                                cTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAg                                                                                                     <  6:285906/149‑1 (MQ=255)                                                   cATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAgg                                                                                                    <  3:284105/147‑1 (MQ=255)                                                    aTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcg                                                                                                                <  2:298581/134‑1 (MQ=255)                                                    aTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcg                                                                                                                >  1:298581/1‑134 (MQ=255)                                                    aTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGcc                                                                                                  >  1:101286/1‑148 (MQ=255)                                                     tGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGa                                                                                                <  4:330144/149‑1 (MQ=255)                                                     tGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGa                                                                                                <  8:324869/149‑1 (MQ=255)                                                       cGTGAAGAAGGCGGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACt                                                                                              <  8:83077/149‑1 (MQ=255)                                                       cGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACt                                                                                              <  7:202344/149‑1 (MQ=255)                                                         tGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCa                                                                                            <  3:99194/149‑1 (MQ=255)                                                                 gCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAgg                                                                                                                       <  5:161993/114‑1 (MQ=255)                                                                 gCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGACCAGCCTCCAAgg                                                                                                                       >  6:161993/1‑114 (MQ=255)                                                                              cTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTg                                                                       >  7:37927/1‑149 (MQ=255)                                                                                    gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATTGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgcg                                                                 <  4:218561/149‑1 (MQ=255)                                                                                    gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                                  <  3:44432/148‑1 (MQ=255)                                                                                    gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                                  >  5:197630/1‑148 (MQ=255)                                                                                    gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                                  >  1:39790/1‑148 (MQ=255)                                                                                    gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAACGCATAGCGCTCGTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                                  >  7:311567/1‑148 (MQ=255)                                                                                     aaCCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgcg                                                                 >  1:180519/1‑148 (MQ=255)                                                                                        cTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAaca                                                             >  3:292804/1‑149 (MQ=255)                                                                                           cATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAacac                                                            <  2:190277/147‑1 (MQ=255)                                                                                           cATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACAcc                                                           <  2:101286/148‑1 (MQ=255)                                                                                             tgggATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTTTCGAGGCCGACTCAGTACCTCTCCGAGCGCGCGAGCCCGCGAACACCgg                                                         >  1:110488/4‑148 (MQ=255)                                                                                               cGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCa                                                       <  3:2931/148‑1 (MQ=255)                                                                                                 aTTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAg                                                    <  4:292804/149‑1 (MQ=255)                                                                                                      gaTGGCAGCGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGt                                               <  7:72071/149‑1 (MQ=255)                                                                                                      gaTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGt                                               <  5:22726/149‑1 (MQ=255)                                                                                                       aTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTg                                              >  7:196281/1‑149 (MQ=255)                                                                                                          gCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGcc                                            <  5:232229/148‑1 (MQ=255)                                                                                                            agagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGccatc                                         >  7:28413/1‑149 (MQ=255)                                                                                                             gagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGc                                                        <  2:220315/133‑1 (MQ=255)                                                                                                             gagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGc                                                        >  1:220315/1‑133 (MQ=255)                                                                                                             gagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCTTTGCCGGTgttcgcg                                        <  2:27581/149‑7 (MQ=39)                                                                                                                      aTCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGt                                <  8:256869/148‑1 (MQ=255)                                                                                                                         gAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGct                            >  5:48816/1‑149 (MQ=255)                                                                                                                           ggCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGcttc                          >  3:349551/1‑149 (MQ=255)                                                                                                                            gCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGcttct                         >  6:32977/1‑149 (MQ=255)                                                                                                                             cGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGcttctt                        <  8:37927/149‑1 (MQ=255)                                                                                                                                 aCTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTcgcg                    >  7:114416/1‑149 (MQ=255)                                                                                                                                 aCTCGATGCCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGAGGTGGCTTCTTcgcg                    >  2:221258/1‑149 (MQ=255)                                                                                                                                  cTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCgg                   <  8:114416/149‑1 (MQ=255)                                                                                                                                    cGATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCggg                  >  1:290417/1‑148 (MQ=255)                                                                                                                                     gATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCACGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGt                 >  6:3197/1‑148 (MQ=255)                                                                                                                                      aTGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTaa               <  2:180519/149‑1 (MQ=255)                                                                                                                                       tGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTaaa              >  1:232672/1‑149 (MQ=255)                                                                                                                                         gCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAAcc            <  5:220079/149‑1 (MQ=255)                                                                                                                                         gCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAAc             <  2:341722/148‑1 (MQ=255)                                                                                                                                          cATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAAccc           >  5:32582/1‑149 (MQ=255)                                                                                                                                              gAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTc       >  8:39333/1‑149 (MQ=255)                                                                                                                                               aGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGt                                >  1:376160/1‑123 (MQ=255)                                                                                                                                               aGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGt                                <  2:376160/123‑1 (MQ=255)                                                                                                                                                   gACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTCCTa    >  1:116909/1‑147 (MQ=255)                                                                                                                                                   gACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTCCTACa  <  3:57195/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTCCTACA  >  NC_002947/81841‑82135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑