Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,414,824 A→G 5.9% K8E (AAG→GAG)  PP_2115 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,414,8240AG 5.9% 174.7 / 2.1 68K8E (AAG→GAG) PP_2115hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/36);  new base G (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

AAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCTGGGTTTGAAAGCC  >  NC_002947/2414680‑2414965
                                                                                                                                                      |                                                                                                                                             
acaCGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGc                                                                                                                                           <  3:15047/147‑1 (MQ=17)
aaaCGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGc                                                                                                                                           <  1:157775/149‑1 (MQ=32)
    gCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGc                                                                                                                                       <  5:334915/149‑1 (MQ=32)
      cttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCAAGGc                                                                                                                                           <  8:75934‑M1/7‑1 (MQ=255)
         gcTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCg                                                                                                                                  <  6:70455/149‑1 (MQ=32)
         gcTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCg                                                                                                                                  <  5:6823/149‑1 (MQ=32)
         gatcttgatcttgatcttgatcttgatcttcgcgagttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatgtatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCAAGGc                                                                                                                                           >  2:202635‑M1/140‑146 (MQ=255)
          cTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCCCGCCCAAGGCCCCATCCAGGGGCCACAGC‑‑‑‑GCATGA‑CGGGC‑ATCCATGCCCGTCACCTGCCATCGCAAGGCCTGCGTTAgg                                                                                                                                 >  2:307230/1‑149 (MQ=11)
           tCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTACCGGCAGGACCGCGCCCAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCATCCCAAGGCCAGCGTTCGGc                                                                                                                                >  8:345666/1‑149 (MQ=11)
             ttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCAAg                                                                                                                                             <  3:118453‑M1/5‑1 (MQ=255)
             ttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCAAg                                                                                                                                             >  4:118453‑M1/136‑140 (MQ=255)
             ccATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGc                                                                                                                                <  4:279396/147‑1 (MQ=32)
              catcatCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGcctc                                                                                                                             >  6:118798/1‑149 (MQ=32)
               atcatcCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGcctcc                                                                                                                            >  7:287328/1‑149 (MQ=32)
                tcatcCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGcctcct                                                                                                                           <  8:221057/149‑1 (MQ=32)
                           ctgcgctgcgCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaa                                                                                                                <  3:162363/149‑1 (MQ=32)
                           ctgcgctgcgCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaa                                                                                                                <  5:118798/149‑1 (MQ=32)
                                   cgctCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaa                                                                                                          <  2:14105/147‑1 (MQ=32)
                                     ctCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGTGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatc                                                                                                      <  5:244506/149‑1 (MQ=17)
                                      tCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatc                                                                                                      >  6:304576/1‑148 (MQ=32)
                                        cGGGGTCCCCTCACTCCGGCCTTTCTCCCGGCAGGACCGCGGCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCAGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatc                                                                                                      <  7:40935/146‑1 (MQ=12)
                                              tcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCGAGGCCGGAGTTCGGCCTCCTGAAGTCGCGaagatcaagatc                                                                                                      <  8:292436‑M1/44‑1 (MQ=255)
                                               cccTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCCGCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑CTCCATGCCCGGCACCTGCCTTCGCAAGGCCGGCGTTCGGCCTCCTGCAGTCGCGcagagcaaggtcaagttcaag                                                                                             >  3:212676/1‑148 (MQ=11)
                                               cccTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaaga                                                                                            >  7:203238/1‑149 (MQ=32)
                                                 cTCAATCCGGCCTTGCTGCCGGCATGACCGCGCCGAAGGCCCCATTCTGGGGGCTCACCCACGGTTTGAGCCACCTATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatc                                                                                                <  1:307230/149‑1 (MQ=2)
                                                      tCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                      >  8:315153/1‑148 (MQ=32)
                                                      tCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCACAAGGCCTGCGTTCGGcctcct                                                                                                                           <  1:133198/111‑1 (MQ=17)
                                                      tCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCACAAGGCCTGCGTTCGGcctcct                                                                                                                           >  2:133198/1‑111 (MQ=17)
                                                        cGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATTCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatc                                                                                    <  7:345666/148‑1 (MQ=17)
                                                         aacgcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCGAGGCCGGAGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaa                                                                                        >  7:292436‑M1/92‑149 (MQ=255)
                                                            gcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCGAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag                                                                                                   >  4:216508‑M1/89‑135 (MQ=255)
                                                            gcaggccttgcggaggcaggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCGAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag                                                                                                   <  3:216508‑M1/47‑1 (MQ=255)
                                                               gCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatca                                                                             <  4:102622/148‑1 (MQ=32)
                                                                  cccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCCTGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatc                                                                              >  1:350513/1‑144 (MQ=17)
                                                                   ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatc                                                                        <  3:218339/149‑1 (MQ=32)
                                                                   ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatc                                                                        <  5:304576/149‑1 (MQ=32)
                                                                   ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagat                                                                         >  8:31099/1‑148 (MQ=32)
                                                                    cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatca                                                                       <  8:287328/149‑1 (MQ=32)
                                                                            ccGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑CCTTGA‑CGGGC‑ATACATGCCCGTCACCTGCCTTCGCAACGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaacatcaagaacaagatcaagaacaacatc                                                                  >  2:109977/1‑146 (MQ=11)
                                                                             aggtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCAAGGCCGGAGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaa                                                                      <  1:172036‑M1/76‑1 (MQ=255)
                                                                               gtgacgggcatggatgcccgtcaagcgctgaggccccaggatggggccttcggcgcggtcctgccgggaGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagat                                                                   <  6:46213‑M1/79‑1 (MQ=255)
                                                                               cgcCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAg                                                                                                                                             >  2:260784/1‑68 (MQ=35)
                                                                               cgcCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAg                                                                                                                                             <  1:260784/68‑1 (MQ=35)
                                                                                   gAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaaga                                                        <  7:31099/149‑1 (MQ=33)
                                                                                         cccATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatc                                                      >  7:152402/1‑145 (MQ=35)
                                                                                           cATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatc                                                <  8:203238/149‑1 (MQ=40)
                                                                                                  gtgCCTCAGC‑‑‑‑GGTTGA‑CGGGC‑ATCCATGCGAGTCACCTGCCTTCGCAAGGCCTGCGTTAGGCCTCCTGAAGTCGCGAAGTTCAAGATCTAGATCAAGATCAAGATCAAGATCTAGATCTAGATCAagatcaagatcaagatcgagatc                                          <  1:109977/146‑1 (MQ=14)
                                                                                                        cAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGGCTCCTGAAGTCCCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAagatcaagatcaagatcgagatcaagata                                    >  1:170364/1‑147 (MQ=255)
                                                                                                         aGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAagatcaagatcaagatcgagatcaagatc                                    >  2:183142/1‑147 (MQ=255)
                                                                                                           c‑‑‑‑gcTTGA‑CGCGC‑ATCCATGCCCGTCACCAGCCGTCGCACCGCCTGCCTTCCGCCTCCAGCAGTCCCCAAGATCAAGATCAAGATCAAGATCAAGAGCAAGATCAACATCAAGATCAagatcaagatcaagatcgagatcaagatcaaga                                <  6:267523/149‑1 (MQ=17)
                                                                                                                gcTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaag                                                               >  4:307740/1‑117 (MQ=32)
                                                                                                                gcTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaag                                                               <  3:307740/117‑1 (MQ=32)
                                                                                                                    gA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAagatcaagatcaagatcgagatcaagatcaagatcg                             <  8:152402/147‑1 (MQ=255)
                                                                                                                     a‑CGGGC‑ATGGATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAggatcaagatcaagatcgagatcaagatcaagatc                              >  2:42965/1‑145 (MQ=255)
                                                                                                                       cGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGAtcaagatcaagatcgagatcaagatcaagatcgaga                          <  1:183142/148‑1 (MQ=255)
                                                                                                                        gggC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCaagatcaagatcgagatcaagatcaagatcgagatc                        <  4:212676/149‑1 (MQ=255)
                                                                                                                                 aTGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAagatcaagatcaagatcgagatcaagatcaaga                                <  7:255201/133‑1 (MQ=255)
                                                                                                                                 aTGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAagatcaagatcaagatcgagatcaagatcaaga                                >  8:255201/1‑133 (MQ=255)
                                                                                                                                 aTGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAgcgc                <  7:315153/149‑1 (MQ=255)
                                                                                                                                 aTGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAgcgc                >  8:228664/1‑149 (MQ=255)
                                                                                                                                 aTGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGAGCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGTTCAAGATCGAGATCAATATCAAGATCGAGATCAAGAgcgc                >  3:37573/1‑149 (MQ=255)
                                                                                                                                  tGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCt               >  1:124542/1‑149 (MQ=255)
                                                                                                                                      cGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCTGGGt           <  2:124542/149‑1 (MQ=255)
                                                                                                                                          aCCTGCCTTCGCAAGGACTGCTTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGTTCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCTGGGTTTGa       <  4:37573/149‑1 (MQ=255)
                                                                                                                                            cTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCTGGGTTTGaaa     >  2:379218/1‑149 (MQ=255)
                                                                                                                                              gCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAagatcaagatcaagatcgaga                                            >  8:92901/1‑108 (MQ=255)
                                                                                                                                              ccgggaGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCTGGGTTTGaaa     >  5:46213‑M1/7‑147 (MQ=255)
                                                                                                                                               ccTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGAGCAAGATCAAGATCAAGATCACGATGAAGGTCGAGGTGAAGATGAAGATCGAGATCAAGAGCGCGGGGTTTGAAAGcg  >  8:79025/1‑148 (MQ=18)
                                                                                                                                                      |                                                                                                                                             
AAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGC‑‑‑‑GCTTGA‑CGGGC‑ATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCGAGATCAAGATCAAGATCGAGATCAAGAGCGCTGGGTTTGAAAGCC  >  NC_002947/2414680‑2414965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑