Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,415,310 A→T 5.5% intergenic (+46/‑15) tusA‑II → / → pdxB sulfurtransferase/erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,415,3100AT 5.5% 226.7 / 2.5 73intergenic (+46/‑15)tusA‑II/pdxBsulfurtransferase/erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base T (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

ACATCCCCAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTGCGCT  >  NC_002947/2415167‑2415457
                                                                                                                                               |                                                                                                                                                   
aCATCCCCAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAg                                                                                                                                                <  6:293211/149‑1 (MQ=255)
 cATCCCCAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAgg                                                                                                                                               <  8:341667/149‑1 (MQ=255)
    ccccAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCAACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGga                                                                                                                                              >  5:234189/1‑147 (MQ=255)
      ccAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAgc                                                                                                                                          >  7:207439/1‑149 (MQ=255)
      ccAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAACAGGACGCAGGCACTTTCCTGTACTGGATCCGCAAGAAAGCAGACTGAATCCCTCTGGAACACGCCCGGCGTTGAGCCGACACTGCGCTCCCCAGACAGGAGAgc                                                                                                                                          >  6:76964/1‑149 (MQ=255)
        aaGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAgcgc                                                                                                                                        >  6:44691/1‑149 (MQ=255)
        aaGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAgcgc                                                                                                                                        >  6:53644/1‑149 (MQ=255)
         aggtCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGcc                                                                                                                                       <  5:127600/146‑1 (MQ=255)
           ttCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCTCCTGTCTGGGGAgcgcagt                                                                                                                                     <  3:288123/149‑4 (MQ=255)
                cAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGc                                                                                                                                   <  2:100482/146‑1 (MQ=255)
                cAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGc                                                                                                                                   <  6:247696/146‑1 (MQ=255)
                cAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGc                                                                                                                                   <  3:289248/146‑1 (MQ=255)
                 aaCTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGa                                                                                                                                >  8:33950/1‑148 (MQ=255)
                  aCTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATa                                                                                                                              <  5:53644/149‑1 (MQ=255)
                      ccTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGtt                                                                                                                           <  2:10689/148‑1 (MQ=255)
                       cTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGc                                                                                                                         >  7:38951/1‑149 (MQ=255)
                       cTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGc                                                                                                                         <  8:353928/149‑1 (MQ=255)
                         gggCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGcc                                                                                                                        >  4:91321/1‑148 (MQ=255)
                          ggCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCcga                                                                                                                      <  1:32407/149‑1 (MQ=255)
                              tcttgctgctGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCcgac                                                                                                                     >  8:143119/5‑146 (MQ=255)
                                 aGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAAc                                                                                                               <  5:259090/149‑1 (MQ=255)
                                  gctgctGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACa                                                                                                              >  2:342818/1‑149 (MQ=255)
                                   ctgctgCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACAt                                                                                                             >  7:117345/1‑149 (MQ=255)
                                    tgctgcAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATc                                                                                                            >  2:357594/1‑149 (MQ=255)
                                            cagcaAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCgctgct                                                                                                    <  7:33950/149‑1 (MQ=255)
                                                aaGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGAt                                                                                                >  2:227985/1‑149 (MQ=255)
                                                    ggACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGACTCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCct                                                                                            <  4:240338/149‑1 (MQ=255)
                                                      aCGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCcttc                                                                                          <  1:227985/149‑1 (MQ=255)
                                                        gCCGGCACTTGCCTGTCCTGGACCCGCCCGAAAGCCGCCTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACCCTGCGCTCCCCAGCCAGTAGAGCGCCATGCTGATAGTTGCCGACGAGCACATCCCGCTGCTCGATGCcttctt                                                                                        <  8:241785/149‑1 (MQ=255)
                                                          cGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTcc                                                                                      <  8:13143/149‑1 (MQ=255)
                                                                    cTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCg                                                                             <  2:330786/148‑1 (MQ=255)
                                                                     tGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGt                                                                           >  7:313964/1‑149 (MQ=255)
                                                                       tACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTg                                                                          >  1:147317/1‑148 (MQ=255)
                                                                       tACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGa                                                                         <  5:44691/149‑1 (MQ=255)
                                                                        aCTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGa                                                                         <  8:117345/148‑1 (MQ=255)
                                                                         cTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGaaa                                                                       <  8:207439/149‑1 (MQ=255)
                                                                         cTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGaaa                                                                       <  8:38951/149‑1 (MQ=255)
                                                                         cTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCATACACTTGGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGaaa                                                                       <  5:76964/149‑1 (MQ=255)
                                                                                cGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTcgcc                                                                 <  1:342818/148‑1 (MQ=255)
                                                                                 gCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGACGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTcgccgc                                                               >  2:198919/1‑149 (MQ=255)
                                                                                                 aaTCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGcc                                               >  8:266927/1‑149 (MQ=255)
                                                                                                  aTCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTCCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCt                                              <  1:357594/149‑1 (MQ=255)
                                                                                                      ctctGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGAt                                          >  1:289696/1‑149 (MQ=255)
                                                                                                      ctctGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGAt                                          >  3:37658/1‑149 (MQ=255)
                                                                                                       tctGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGAtg                                         >  6:64552/1‑149 (MQ=255)
                                                                                                         tGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGAtgct                                       >  4:224906/1‑149 (MQ=255)
                                                                                                           gAACATGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGAtgctgc                                     >  3:26164/1‑149 (MQ=255)
                                                                                                                 gCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATTCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGt                               <  8:329307/149‑1 (MQ=255)
                                                                                                                 gCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGATGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACACCGGCCGAAGCCTCGATGCTGCCAGCGt                               >  7:88942/1‑149 (MQ=255)
                                                                                                                  cccGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGt                               >  3:359385/1‑148 (MQ=255)
                                                                                                                    cGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTc                              >  1:260223/1‑147 (MQ=255)
                                                                                                                    cGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTc                              >  8:295452/1‑147 (MQ=255)
                                                                                                                    cGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCaa                            <  3:91321/149‑1 (MQ=255)
                                                                                                                    cGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCaa                            <  7:143119/149‑1 (MQ=255)
                                                                                                                    cGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCaa                            <  4:37658/149‑1 (MQ=255)
                                                                                                                     ggCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCGAGGGTTTCGGTGAAATTCGCCGCTTCCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAg                           <  8:313964/149‑1 (MQ=255)
                                                                                                                         ttGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACg                       >  6:5790/1‑149 (MQ=255)
                                                                                                                           gcgcCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGc                      <  4:26164/148‑1 (MQ=255)
                                                                                                                             gcCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGa                   <  2:260223/149‑1 (MQ=255)
                                                                                                                                 acacTGCGCTCCCCAGACAGGAGAGCGCCAAGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATc               >  1:32229/1‑149 (MQ=255)
                                                                                                                                  cacTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATc               <  4:359385/148‑1 (MQ=255)
                                                                                                                                   acTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCct             >  6:251245/1‑149 (MQ=255)
                                                                                                                                    tggcgcTCTCCTGTCTGGGGAGCGCCATGCTGATAGTTGCAGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCctg            >  5:4555/3‑149 (MQ=255)
                                                                                                                                      gcgcTCTCCTGTCTGGGGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCctg            <  4:233638/147‑1 (MQ=255)
                                                                                                                                      gcgcTCTCCTGTCTGGGGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCctg            >  3:233638/1‑147 (MQ=255)
                                                                                                                                       cgcTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCctgct          <  2:289696/148‑1 (MQ=255)
                                                                                                                                         cTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGt       <  5:5790/149‑1 (MQ=255)
                                                                                                                                          tCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTg      >  1:218484/1‑149 (MQ=255)
                                                                                                                                            cccAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTgcg    >  2:14578/1‑149 (MQ=255)
                                                                                                                                            cccAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTgcg    >  6:136428/1‑149 (MQ=255)
                                                                                                                                             ccAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTgcgc   >  1:170482/1‑149 (MQ=255)
                                                                                                                                             ccAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTgcgc   >  1:164879/1‑149 (MQ=255)
                                                                                                                                              cAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTGCGCt  >  1:210909/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ACATCCCCAAGTTCTGCAACTTCCTGGGCCACGAGCTGCTGCAGCAGCAAGAGGACGCCGGCACTTTCCTGTACTGGATCCGCAAGAAAGCCGACTGAATCGCTCTGGAACACGCCCGGCGTTGCGCCTACACTGCGCTCCCCAGACAGGAGAGCGCCATGCTGATAGTTGCCGACGAGAACATCCCGCTGCTCGATGCCTTCTTCCAGGGTTTCGGTGAAATTCGCCGCTACCCCGGCCGAAGCCTCGATGCTGCCAGCGTCAAGGACGCCGATATCCTGCTGGTGCGCT  >  NC_002947/2415167‑2415457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑