Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,443,502	T→A	8.3%	F6L (TTT→TTA) ‡	PP_2140 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,443,502	0	T	A	8.3%	202.4 / 4.7	70	F6L (TTT→TTA) ‡	PP_2140	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base A (4/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TGATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAA  >  NC_002947/2443354‑2443643                                                                                                                                                     |                                                                                                                                              tGATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCttt                                                                                                                                               <  8:49723/149‑1 (MQ=255)   aTCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTg                                                                                                                                              <  3:249203/148‑1 (MQ=255)    tCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATACTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCg                                                                                                                                            >  1:296437/1‑149 (MQ=255)      cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGTTTTGGGGCCGCGTTTCGGc                                                                                                                                          >  1:159651/1‑149 (MQ=255)      cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                           <  5:151168/148‑1 (MQ=255)      cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                           <  6:72469/148‑1 (MQ=255)        cTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGGCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGGTGAAGATAAGCGCTGAGCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGccc                                                                                                                                        <  5:314112/149‑1 (MQ=255)           cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaa                                                                                                                                     <  3:230237/149‑1 (MQ=255)               cGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGCCCGGCGGGAAGGCATTCTACGACGGCAATGCCTGGAAGGGTGAAGATAAGCGCTCATCCATTGGTGCTGTGATTTGGGGCCGCGTTGCGGCCCCAATCAt                                                                                                                                 <  7:74158/149‑1 (MQ=255)               cGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAt                                                                                                                                 <  3:125920/149‑1 (MQ=255)               cGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAt                                                                                                                                 <  3:232633/149‑1 (MQ=255)                 cAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                >  2:173733/1‑148 (MQ=255)                        aaGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaa                                                                                                                                    <  3:372248/137‑2 (MQ=255)                        aaGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaa                                                                                                                                    >  4:372248/1‑136 (MQ=255)                         aGGAGCAGTATGTGCAGTCCGAGGTAGATGGCAAGCCGACCGGATGGAAGGCATTCTACGACGGAAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                       >  7:211504/1‑149 (MQ=255)                          ggAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAttt                                                                                                                               >  4:173819/1‑140 (MQ=255)                          ggAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAttt                                                                                                                               <  3:173819/140‑1 (MQ=255)                              cAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGatat                                                                                                                  >  2:145160/1‑149 (MQ=255)                                      gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                          >  6:283988/1‑149 (MQ=255)                                       cAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGc                                                                                                                                             >  5:136944/1‑113 (MQ=255)                                       cAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGc                                                                                                                                             <  6:136944/113‑1 (MQ=255)                                         gTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAg                                                                                                       >  3:350826/1‑149 (MQ=255)                                          tCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                      >  6:31522/1‑149 (MQ=255)                                                gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTAt                                                                                                 <  8:97985/148‑1 (MQ=255)                                                gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATg                                                                                                <  6:7205/149‑1 (MQ=255)                                                   gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTAATGGGa                                                                                             <  4:195135/149‑1 (MQ=255)                                                    aTGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAg                                                                                            >  6:99821/1‑149 (MQ=255)                                                        cAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCa                                                                                        >  4:242920/1‑149 (MQ=255)                                                         aatccGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGACCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCAc                                                                                       <  8:211504/146‑1 (MQ=255)                                                          aGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                      <  4:350826/149‑1 (MQ=255)                                                           gCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                      >  8:107384/1‑148 (MQ=255)                                                               aCCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                      <  6:135250/128‑1 (MQ=255)                                                               aCCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                      >  5:135250/1‑128 (MQ=255)                                                                     tGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                           <  7:142985/149‑1 (MQ=255)                                                                     gggAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                           <  6:261461/148‑1 (MQ=255)                                                                      ggAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                          >  8:168029/1‑149 (MQ=255)                                                                       gAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                           <  7:107384/147‑1 (MQ=255)                                                                       gAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                          <  5:31522/148‑1 (MQ=255)                                                                       gAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                          <  7:115667/148‑1 (MQ=255)                                                                       gAAGGCATCCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                           <  2:296437/147‑1 (MQ=255)                                                                         aGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGaa                                                                       >  4:348306/1‑149 (MQ=255)                                                                             aTTCTAGGACGGCAATGCCGGGAAGGTTGAAGATACCCGCTGATCCATTGGGGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActct                                                                   <  2:167235/149‑1 (MQ=255)                                                                             aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActct                                                                   <  1:173733/149‑1 (MQ=255)                                                                              ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTa                                                                  <  5:99821/149‑1 (MQ=255)                                                                                    gacgGCAATGCGTGGATGGTTGAAGATAAGCGCTGATCCATTCGTTCTGTGATTTGGGGCCGCCTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                            <  1:304526/149‑1 (MQ=255)                                                                                    gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                            >  8:38923/1‑149 (MQ=255)                                                                                        gCAATGCCTGGAAGGTTGAAGATAAGCGCTGCTCCATTGGTGCTGTGATTTGGGGCCGCTGTGCGGCCCCAATCATTTATGATGCAGATAGCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACCCCCGTac                                                         >  6:127929/1‑148 (MQ=255)                                                                                           aTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                     >  5:33235/1‑149 (MQ=255)                                                                                           aTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCACCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                     >  5:38602/1‑149 (MQ=255)                                                                                               cTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAg                                                  >  8:51444/1‑148 (MQ=255)                                                                                                  gAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                               <  7:51444/148‑1 (MQ=255)                                                                                                   aaGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACt                                             <  3:242920/149‑1 (MQ=255)                                                                                                      gTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGtt                                          <  5:283988/149‑1 (MQ=255)                                                                                                          aaGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttcttc                                      <  1:145160/149‑1 (MQ=255)                                                                                                            gATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATTGCCCAGGAAc                                                                      >  3:249972/1‑115 (MQ=255)                                                                                                            gATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATTGCCCAGGAAc                                                                      <  4:249972/115‑1 (MQ=255)                                                                                                                   gcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCACCCAGAAACTGTTCTTCGCCGGCCTc                             >  5:96634/1‑149 (MQ=255)                                                                                                                     tGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGc                           >  7:92983/1‑149 (MQ=255)                                                                                                                       aTCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGccc                         >  4:228995/1‑149 (MQ=255)                                                                                                                          cATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                       <  7:168029/148‑1 (MQ=255)                                                                                                                           aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                       >  3:29660/1‑147 (MQ=255)                                                                                                                                  taaatgattGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATg              >  1:334295/8‑149 (MQ=255)                                                                                                                                   tgtgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATTTCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCTGAACCTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg             >  3:71285/1‑149 (MQ=255)                                                                                                                                    gtgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCGGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGcc                                   <  1:120726/126‑1 (MQ=255)                                                                                                                                    gtgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGcc                                   >  2:120726/1‑126 (MQ=255)                                                                                                                                     tgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg             <  3:228995/147‑1 (MQ=255)                                                                                                                                     tgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGc            <  4:29660/148‑1 (MQ=255)                                                                                                                                       gattGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc           >  1:195048/3‑147 (MQ=255)                                                                                                                                        tttGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCaa         <  3:348306/148‑1 (MQ=255)                                                                                                                                         ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAg        >  7:187164/1‑148 (MQ=255)                                                                                                                                              gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGaa  >  4:286346/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                              TGATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAA  >  NC_002947/2443354‑2443643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑