Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,443,848 G→A 100% A122T (GCC→ACC)  PP_2140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,443,8480GA100.0% 168.0 / NA 51A122T (GCC→ACC) PP_2140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/24);  total (27/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCACCTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGGCCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCAT  >  NC_002947/2443703‑2443988
                                                                                                                                                 |                                                                                                                                            
ccACCTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGAccc                                                                                                                                           >  3:243635/1‑149 (MQ=255)
 cACCTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGAccc                                                                                                                                           <  4:345896/148‑1 (MQ=255)
  aCCTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGc                                                                                                                                         >  6:212445/1‑149 (MQ=255)
    cTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGc                                                                                                                                         >  3:270511/1‑147 (MQ=255)
    cTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGc                                                                                                                                         >  3:344368/1‑147 (MQ=255)
         tGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGTAGCAAGCCCTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCgtg                                                                                                                                  <  4:288136/149‑3 (MQ=255)
            cgcTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGaaa                                                                                                                               <  5:225195/149‑1 (MQ=255)
                 tGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCCGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACctg                                                                                                                           <  4:100297/148‑1 (MQ=255)
                        ccTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGgcgc                                                                                                                   <  5:212445/149‑1 (MQ=255)
                              ccATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGc                                                                                                             >  4:167559/1‑149 (MQ=255)
                               cATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCt                                                                                                            >  7:134205/1‑149 (MQ=255)
                               cATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCt                                                                                                            <  7:235605/149‑1 (MQ=255)
                               cATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCt                                                                                                            >  5:187974/1‑149 (MQ=255)
                                aTGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTc                                                                                                           <  4:270511/149‑1 (MQ=255)
                                aTGAAATCGGGGGCTTCTACCGCCTGACGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGCAAGTGGCCGAGTTGCTGCAGCTGACCCGCCAGAGGGAAACATGGCTGGCGCAAATGCTc                                                                                                           >  6:227572/1‑149 (MQ=255)
                                         ggggCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTa                                                                                                   <  3:255944/148‑1 (MQ=255)
                                         ggggCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTa                                                                                                   >  4:255944/1‑148 (MQ=255)
                                          gggCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTc                                                                                                           <  4:358854/139‑1 (MQ=255)
                                          gggCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTc                                                                                                           >  3:358854/1‑139 (MQ=255)
                                           ggCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGc                                                                                                >  3:225902/1‑149 (MQ=255)
                                             cTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGcga                                                                                              <  4:344368/149‑3 (MQ=255)
                                             cTTCTACCGACTGCAGGTAGTAGAAACGGTGGAGCAAGCACTTGCCGACGATGCCCTGAACGGTATAGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGACAGCGGGAAACCTGGCTGGCGCAAATGCTAGGTGCTTAtccg                                                                                               >  8:96262/1‑145 (MQ=255)
                                                cTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGAt                                                                                            <  5:275317/148‑1 (MQ=255)
                                                   ccGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATGCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGt                                                                                        >  4:201658/1‑149 (MQ=255)
                                                   ccGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTg                                                                                         <  3:180905/148‑1 (MQ=255)
                                                          ccGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCg                                                                                                          <  6:378359/124‑1 (MQ=255)
                                                          ccGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCg                                                                                                          >  5:378359/1‑124 (MQ=255)
                                                                   gCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGc                                                                        >  6:185572/1‑149 (MQ=255)
                                                                                     cTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAAc                                                      >  1:242104/1‑149 (MQ=255)
                                                                                        gCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAAcc                                                     <  6:187974/147‑1 (MQ=255)
                                                                                        gCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGa                                                   >  8:88141/1‑149 (MQ=255)
                                                                                         ccGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGAc                                                  <  4:225902/149‑1 (MQ=255)
                                                                                         ccGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGAc                                                  <  4:133477/149‑1 (MQ=255)
                                                                                          cgacgaTGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACg                                                 <  2:242104/149‑1 (MQ=255)
                                                                                                   ccTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGc                                        >  3:271238/1‑149 (MQ=255)
                                                                                                    cTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGc                                                                                                             >  2:360642/1‑79 (MQ=255)
                                                                                                    cTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGc                                                                                                             <  1:360642/79‑1 (MQ=255)
                                                                                                      gAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGcccc                                     >  2:326199/1‑149 (MQ=255)
                                                                                                            tATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGAtt                                >  4:312056/1‑148 (MQ=255)
                                                                                                            tATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGAtt                                >  1:303498/1‑148 (MQ=255)
                                                                                                              tCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCaa                             <  4:243635/149‑1 (MQ=255)
                                                                                                               cGCCATCCCGGAAGTGGACGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCaa                             <  5:227572/148‑1 (MQ=255)
                                                                                                                gCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGc                           >  7:204825/1‑149 (MQ=255)
                                                                                                                  cATCCCGGAAGTGGCCGAGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTg                         >  5:99019/1‑149 (MQ=255)
                                                                                                                               gCCGAGTTGCTGGAGCTGACCCGAGAGGGTGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCGGATTTGTTCCGACGACCGGTCGCCTGAAATGCGCCGAAAAGCGACGTTAGCCAGCCCGTGATTCaa                             <  7:99475/132‑1 (MQ=255)
                                                                                                                                   aGTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGcat        >  8:212278/1‑147 (MQ=255)
                                                                                                                                    gTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGccc                                      >  5:242235/1‑118 (MQ=255)
                                                                                                                                    gTTGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGccc                                      <  6:242235/118‑1 (MQ=255)
                                                                                                                                     ttGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGa      <  3:167559/149‑1 (MQ=255)
                                                                                                                                     ttGCTGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGa      <  8:134205/149‑1 (MQ=255)
                                                                                                                                         tGGAGCTGACCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCAt  >  1:246295/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CCACCTGTATGGCGCTCTGTTGGGCCTGTGCCATGAAATCGGGGGCTTCTACCGCCTGCCGGTCGTCGCAACGGTGGAGCAAGCACTGGCCGACGATGCCCTGAACGGTATCGCCATCCCGGAAGTGGCCGAGTTGCTGGAGCTGGCCCGCCAGCGGGAAACCTGGCTGGCGCAAATGCTCGGTGCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCAT  >  NC_002947/2443703‑2443988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑