Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	255,264	A→G	7.2%	intergenic (‑148/+15)	PP_0203 ← / ← PP_t74	dipeptidase/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	255,264	0	A	G	7.2%	199.3 / 3.6	69	intergenic (‑148/+15)	PP_0203/PP_t74	dipeptidase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (33/31);  new base G (2/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCTGTG  >  NC_002947/255118‑255410                                                                                                                                                     |                                                                                                                                                    gTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAgg                                                                                                                                                   <  2:29511/149‑1 (MQ=255)      gcgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCtt                                                                                                                                               >  4:50828/1‑148 (MQ=255)       cgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAg                                                                                                                                                    >  2:44126/1‑142 (MQ=255)       cgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAg                                                                                                                                                    <  1:44126/142‑1 (MQ=255)         cATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAG‑C‑CCGGGGCtttttc                                                                                                                                           <  3:256176/149‑4 (MQ=255)         cATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTAtt                                                                                                                                           <  6:124227/149‑1 (MQ=255)             ccTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAG‑C‑CCGGGGCtttttcgtt                                                                                                                                        <  2:209706/148‑7 (MQ=255)               tttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATg                                                                                                                                     >  5:180488/1‑149 (MQ=255)               tttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATg                                                                                                                                     >  2:183975/1‑149 (MQ=255)                    cAATCATTGTTCAGGGTTACCCGTTCGTGAAAAGCGAAGACGCCCACGCTAACACCAATCACCCGTCGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACCAAAAAGCCCCGGCATCAGG‑C‑CCAGGGCTTTATTGATTTGgcgc                                                                                                                                 >  7:209428/1‑148 (MQ=255)                    cAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCa                                                                                                                                >  5:131828/1‑149 (MQ=255)                     aaTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCAc                                                                                                                               >  2:128870/1‑149 (MQ=255)                      aTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACt                                                                                                                              >  2:277105/1‑149 (MQ=255)                             ttCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTcggcgg                                                                                                                        <  1:277105/148‑1 (MQ=255)                               cAGGGTTACGCGTTCGGGAAAGGGGAAGACGCCCACGCTAAGACGAATGACCCGTGGTACGGGTGTTGCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGTGCTCGGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGa                                                                                                                      <  6:335680/148‑1 (MQ=255)                                 gggTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTc                                                                                                                   <  6:21773/149‑1 (MQ=255)                                     tACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGaa                                                                                                                >  4:246765/1‑148 (MQ=255)                                        ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                            >  2:227225/1‑149 (MQ=255)                                        ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                            >  8:49687/1‑149 (MQ=255)                                          gTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                           >  6:364566/1‑148 (MQ=255)                                          gTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                           >  4:201922/1‑148 (MQ=255)                                              gggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAGCCCACGAccc                                                                                                      >  6:274845/1‑149 (MQ=255)                                                gAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑t                                                                                                   <  3:277398/149‑1 (MQ=255)                                                  aaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGc                                                                                                 >  7:29129/1‑149 (MQ=255)                                                  aaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGc                                                                                                 >  7:23328/1‑149 (MQ=255)                                                   aCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑ACAGGGATTTATTGATATGGCGCAATCGGCGGGATTCGAAACACCGACCCC‑TGc                                                                                                 >  3:97709/1‑148 (MQ=255)                                                    cGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCt                                                                                               <  1:227225/149‑1 (MQ=255)                                                       gAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCg                                                                                            >  8:291871/1‑149 (MQ=255)                                                         aGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGa                                                                                          <  5:364566/149‑1 (MQ=255)                                                          gACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAg                                                                                         <  3:201922/149‑1 (MQ=255)                                                          gACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAg                                                                                         <  2:123246/149‑1 (MQ=255)                                                              cccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCa                                                                                     <  3:323851/149‑1 (MQ=255)                                                              cccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCa                                                                                     <  7:291871/149‑1 (MQ=255)                                                               ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAg                                                                                    >  2:53124/1‑149 (MQ=255)                                                                cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTcgg                                                                                                                           <  4:73574/110‑1 (MQ=255)                                                                cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTagg                                                                                                                           >  3:73574/1‑110 (MQ=255)                                                                  cGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTAc                                                                                 <  7:49687/149‑1 (MQ=255)                                                                     tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGatat                                                                                                                                      <  3:80020/94‑1 (MQ=255)                                                                     tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGatat                                                                                                                                      >  4:80020/1‑94 (MQ=255)                                                                      aaCACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTa                                                                             >  4:87775/1‑149 (MQ=255)                                                                        cacCAATGACGCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATc                                                                           <  2:174158/149‑1 (MQ=255)                                                                         acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATcc                                                                          <  5:4713/149‑1 (MQ=255)                                                                           cAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCC‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTc                                                                                                                   <  1:247388/108‑1 (MQ=255)                                                                             aTGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                      <  1:128870/149‑1 (MQ=255)                                                                                  ccGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGc                                                                  >  6:95076/1‑148 (MQ=255)                                                                                       gTACTGGAGTACCAGAAAGGTGACATGTCGCACTATCGATAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACgt                                                             <  8:209428/148‑2 (MQ=255)                                                                                       gTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGa                                                             <  6:131828/148‑1 (MQ=255)                                                                                          cGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTg                                                          <  5:343885/148‑1 (MQ=255)                                                                                          cGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGc                                                         <  6:180488/149‑1 (MQ=255)                                                                                             gAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCATGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAc                                                      <  1:183975/149‑1 (MQ=255)                                                                                              aGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAcg                                                     <  3:50828/149‑1 (MQ=255)                                                                                                    aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑CTCAAGGGCTTTATTGATATGGCGCACACGGCGGGATTCGAACCCACGACCCC‑TGCCTGCGGAGGGCAGTACTCTAGCCAGATGAGCTACGAGTCCAACgcgccc                                                >  1:268015/1‑147 (MQ=255)                                                                                                    aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacg                                               >  1:70982/1‑149 (MQ=255)                                                                                                                     cacaAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtgtg                              >  4:311591/1‑149 (MQ=255)                                                                                                                      acaAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtg                                <  8:30047/146‑1 (MQ=255)                                                                                                                      acaAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtg                                >  7:30047/1‑146 (MQ=255)                                                                                                                      acaAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                             <  8:29129/149‑1 (MQ=255)                                                                                                                      acaAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                             <  8:23328/149‑1 (MQ=255)                                                                                                                      acaAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                             <  2:268015/149‑1 (MQ=255)                                                                                                                        aaaCGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCg                           >  6:222203/1‑149 (MQ=255)                                                                                                                          tcaataaaGCCCTGGGCCTGAG‑C‑CCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                             >  3:190524/6‑145 (MQ=38)                                                                                                                          tcaataaaGCCCTGGGCCTGAG‑C‑CCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                             <  4:190524/140‑1 (MQ=38)                                                                                                                          aCGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGtt                         >  8:337868/1‑149 (MQ=255)                                                                                                                               aaaGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt                    <  3:87775/149‑1 (MQ=255)                                                                                                                                     ccGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCg              >  3:285526/1‑149 (MQ=255)                                                                                                                                         gCTAAGG‑C‑CCAGGGCTTAATTGATATGGCGCACTCGGCGGGATTCGAAACCACGACCCC‑TGCCTTCGGACGCCAGTACTCTAACCAGCTGAGCTACGAGTGAAACGCGCACGAATGATACCCATCTGTCTCGTTGAAGTCCATCGccat          >  6:82074/1‑147 (MQ=255)                                                                                                                                             aGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATc      >  5:256386/1‑149 (MQ=255)                                                                                                                                               g‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCACGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCt     >  1:98931/1‑149 (MQ=255)                                                                                                                                                   ccAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCtgtg  >  7:130541/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGG‑C‑CCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCC‑TGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCTGTG  >  NC_002947/255118‑255410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑