Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,469,844 G→A 15.0% intergenic (+13/+22) vacJ → / ← PP_2164 lipoprotein VacJ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,469,8440GA15.0% 139.6 / 13.9 60intergenic (+13/+22)vacJ/PP_2164lipoprotein VacJ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/21);  new base A (4/5);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCT  >  NC_002947/2469699‑2469990
                                                                                                                                                 |                                                                                                                                                  
gATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGc                                                                                                                                                 >  7:292883/1‑149 (MQ=255)
         ccGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGctttgcg                                                                                                                                        >  3:209175/1‑143 (MQ=255)
         ccGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCg                                                                                                                                        >  3:88415/1‑149 (MQ=255)
              ccAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTTCCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTc                                                                                                                                   <  1:102444/149‑1 (MQ=255)
               cAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCg                                                                                                                                  >  2:208707/1‑149 (MQ=255)
               cAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACAGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGCCTTCTGCTCTGTGAACCTGGGGCCGCAAAGCGGCCTCg                                                                                                                                  >  3:94057/1‑149 (MQ=255)
                 gCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCggg                                                                                                                                <  5:277731/149‑1 (MQ=255)
                 gCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCCGAGGCCGCTTTGCGGccccagg                                                                                                                                <  4:368918/149‑6 (MQ=255)
                       tctcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAAt                                                                                                                          >  7:51575/1‑149 (MQ=255)
                       tctcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTATGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAAt                                                                                                                          >  7:56188/1‑149 (MQ=255)
                         tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGCAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCCGAGGCCGCTTTGCGGCCCCAGGTTCaca                                                                                                                          <  7:169672/147‑3 (MQ=38)
                         tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATg                                                                                                                         >  5:214906/1‑148 (MQ=255)
                         tcGGCCGAAAAGCTGATTCAGGGGGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCCGAGGCCGCTTTGCGGCCCCAGGTTCaca                                                                                                                          <  5:292742/147‑3 (MQ=38)
                           ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCAt                                                                                                                      >  4:803/1‑149 (MQ=255)
                           ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCCGAGGCCGCTTTGCGGCCCCAGGTTCacaga                                                                                                                        <  5:330181/147‑5 (MQ=38)
                           ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCCGAGGCCGCTTTGCGGCCCCAGGTTCac                                                                                                                           >  3:141296/1‑143 (MQ=38)
                           ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCCGAGGCCGCTTTGCGGCCCCAGGTTCac                                                                                                                           <  4:141296/144‑2 (MQ=38)
                            gCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCTTTGCGGCCCCAGGTTCacagatca                                                                                                                     <  4:209175/149‑8 (MQ=255)
                             ccGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATgg                                                                                                                    <  6:239243/149‑1 (MQ=255)
                             ccGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATgg                                                                                                                    <  8:82205/149‑1 (MQ=255)
                                aaaaGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                   >  1:219137/1‑147 (MQ=255)
                                   aGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGaa                                                                                                              <  2:181716/149‑1 (MQ=255)
                                    gCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGAAGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAAt                                                                                                             >  3:56322/1‑149 (MQ=255)
                                    gCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATCCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTgtgg                                                                                                                                                  >  6:300940/1‑112 (MQ=255)
                                        aTTCAGGGTTACAATTACATCTTCATTCTCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCTGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCg                                                                                                          <  8:56188/148‑1 (MQ=255)
                                        aTTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCg                                                                                                          <  8:51575/148‑1 (MQ=255)
                                        aTTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGc                                                                                                         <  1:314543/149‑1 (MQ=255)
                                                  aCAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAAc                                                                                                <  2:219137/148‑1 (MQ=255)
                                                         cATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGAGCCGCAAACCGGCCTCGGGTTc                                                                                                                             >  8:351177/1‑112 (MQ=255)
                                                             ttCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcccg                                                                                    >  1:199708/1‑149 (MQ=255)
                                                                 ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCgg                                                                                                                                 <  5:235677/100‑1 (MQ=255)
                                                                 ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCgg                                                                                                                                 >  6:235677/1‑100 (MQ=255)
                                                                 ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGcccgcccg                                                                                >  7:117628/1‑149 (MQ=255)
                                                                          ccTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCg                                                                        <  8:292883/148‑1 (MQ=255)
                                                                                 gCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGAGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATcc                                                                 >  8:116830/1‑148 (MQ=255)
                                                                                  cAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCAt                                                               <  2:199708/149‑1 (MQ=255)
                                                                                  cAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGCGCCGCAAAGCGGCCTCGGGTCCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                <  7:242107/148‑1 (MQ=255)
                                                                                         gcgAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACtt                                                        >  8:98547/1‑149 (MQ=255)
                                                                                         gcgAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACtt                                                        >  4:179580/1‑149 (MQ=255)
                                                                                         gcgAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACtt                                                        >  5:125572/1‑149 (MQ=255)
                                                                                                 aaGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCt                                                 <  1:208707/148‑1 (MQ=255)
                                                                                                     tGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCAt                                            <  6:125572/149‑1 (MQ=255)
                                                                                                           aTGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcc                                      >  5:383698/1‑149 (MQ=255)
                                                                                                              gCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCGCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc                                    <  4:88415/148‑1 (MQ=255)
                                                                                                                    tCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAgcg                             >  3:2780/1‑149 (MQ=255)
                                                                                                                      gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                           <  3:179580/149‑1 (MQ=255)
                                                                                                                            gacTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATg                      >  7:271956/1‑148 (MQ=255)
                                                                                                                              cTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGa                    <  8:117628/148‑1 (MQ=255)
                                                                                                                               ttCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                >  4:204596/1‑119 (MQ=255)
                                                                                                                               ttCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                <  3:204596/119‑1 (MQ=255)
                                                                                                                               ttCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCAGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACg                  >  5:359644/1‑149 (MQ=255)
                                                                                                                                  tGATCTGTGAACCTGGGGCCGCGAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAg               >  8:18232/1‑149 (MQ=255)
                                                                                                                                       tgtgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGAt          >  1:150380/1‑149 (MQ=255)
                                                                                                                                       attgAACCCGAGGCCGCTTTGCGGCCCCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGAt          >  7:190095/3‑149 (MQ=255)
                                                                                                                                       attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGAt          >  3:313339/3‑149 (MQ=38)
                                                                                                                                       attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGAt          >  8:219617/3‑149 (MQ=38)
                                                                                                                                        gtgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATc         >  1:116064/1‑149 (MQ=255)
                                                                                                                                         cgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATcc        <  7:116830/148‑1 (MQ=255)
                                                                                                                                           aaCCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCg       >  5:267133/1‑148 (MQ=255)
                                                                                                                                             ccTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTAc    >  1:63658/1‑149 (MQ=255)
                                                                                                                                              cTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTAcc   >  1:224405/1‑149 (MQ=255)
                                                                                                                                                 gggCCGCAACCCGGCCGCGGGCTCAATGCATGGCCAGAATCCGCAGCCCGCACCTCTGCTGCCCGCCCGGCGGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCt  <  6:214906/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCT  >  NC_002947/2469699‑2469990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑