Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	262,752	T→A	6.7%	L90Q (CTG→CAG)	PP_0211 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	262,752	0	T	A	6.7%	203.8 / 2.9	69	L90Q (CTG→CAG)	PP_0211	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base A (2/3);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGA  >  NC_002947/262606‑262900                                                                                                                                                   |                                                                                                                                                      ccGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTgg                                                                                                                                                     <  5:8144/149‑1 (MQ=255)  cGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGt                                                                                                                                                    <  8:19905/149‑1 (MQ=255)  cGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGt                                                                                                                                                    <  8:250513/149‑1 (MQ=255)        gcgcgcCTGTCCGTCGGGATCCGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCacgcaa                                                                                                                                             <  8:301925/149‑6 (MQ=255)        gcgcgcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCaa                                                                                                                                             <  4:265740/149‑1 (MQ=255)        gcgcgcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCacgcaa                                                                                                                                             <  3:67939/149‑6 (MQ=255)          gcgcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCACGCAAGt                                                                                                                                           <  7:322079/149‑1 (MQ=255)           cgcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGt                                                                                                                                           >  5:265236/1‑148 (MQ=255)               tGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGc                                                                                                                                      >  5:214263/1‑149 (MQ=255)                   cGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGa                                                                                                                                   <  3:11193/148‑1 (MQ=255)                    ggAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGTGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATc                                                                                                                                 >  8:224458/1‑149 (MQ=255)                    ggAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATc                                                                                                                                 >  6:97245/1‑149 (MQ=255)                    ggAGGGATCACCCAGGTGCGCGTCGATGTGCGCGTGGTCCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGAGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTCGTCA‑GCAAGTGCACCTGATc                                                                                                                                 >  2:48526/1‑149 (MQ=255)                     gAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑Gc                                                                                                                                               >  4:347611/1‑134 (MQ=255)                     gAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑Gc                                                                                                                                               <  3:347611/134‑1 (MQ=255)                      aGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATccc                                                                                                                               <  2:99044/149‑1 (MQ=255)                      aGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATccc                                                                                                                               <  4:305043/149‑1 (MQ=255)                           tCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAg                                                                                                                          >  3:286008/1‑149 (MQ=255)                               ccTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGc                                                                                                                      <  8:35401/149‑1 (MQ=255)                                cTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGcc                                                                                                                     <  6:241206/149‑1 (MQ=255)                                cTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGcc                                                                                                                     <  7:168197/149‑1 (MQ=255)                                    tGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCgg                                                                                                                   >  6:157448/1‑147 (MQ=255)                                       gcgACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                              <  2:189660/149‑1 (MQ=255)                                        cgacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTAGCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCGCTTCAGTTGACGGAAgggg                                                                                                             >  8:136454/1‑149 (MQ=255)                                         gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGc                                                                                                                                      >  3:294412/1‑123 (MQ=255)                                         gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGc                                                                                                                                      <  4:294412/123‑1 (MQ=255)                                         gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGt                                                                                                            >  8:40203/1‑149 (MQ=255)                                           cgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGTGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGc                                                                                                          <  7:224458/149‑1 (MQ=255)                                                  cgcgTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCa                                                                                                   <  8:150226/149‑1 (MQ=255)                                                   gcgTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAg                                                                                                  >  4:194610/1‑149 (MQ=255)                                                            aaaTCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGc                                                                                         >  7:200351/1‑149 (MQ=255)                                                             aaTCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGcc                                                                                        >  8:163761/1‑149 (MQ=255)                                                                 gAGTCGCAAGTGTATTGCTTACAACTGGTGTTCAGCGATTGGCATGAGCGCGGGATCTACCCTTGTGATTACTTGCGTTACCTTGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCTCCTAGc                                                                                    <  8:115097/149‑1 (MQ=255)                                                                 gAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGc                                                                                    <  5:97245/149‑1 (MQ=255)                                                                 gAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGc                                                                                    <  6:214263/149‑1 (MQ=255)                                                                      gCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGa                                                                                                                                   >  7:332415/1‑97 (MQ=255)                                                                      gCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGa                                                                                                                                   <  8:332415/97‑1 (MQ=255)                                                                      gCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAAc                                                                                >  7:187093/1‑148 (MQ=255)                                                                       cAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACg                                                                               >  1:91201/1‑148 (MQ=255)                                                                             tATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGc                                                                        <  6:265236/149‑1 (MQ=255)                                                                                  cGTACAACTGGTGTTCAGCTATGTGCATGAGCGCGGGATCTACCCTTGTGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCATTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                                   <  5:268378/149‑1 (MQ=255)                                                                                  cGTACAACTGGTGTTCAGCGATGGGCATGAGCGCTGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                                   <  5:379613/149‑1 (MQ=255)                                                                                  cGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                                   <  8:187093/149‑1 (MQ=255)                                                                                   gTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCtt                                                                                                                             >  2:305991/1‑90 (MQ=255)                                                                                   gTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCtt                                                                                                                             <  1:305991/90‑1 (MQ=255)                                                                                       aaCTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAAc                                                              >  8:60461/1‑149 (MQ=255)                                                                                       aaCTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAAc                                                              <  3:115763/149‑1 (MQ=255)                                                                                              gtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGctct                                                       >  4:112914/1‑149 (MQ=255)                                                                                                  aGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTg                                                    <  8:200351/148‑1 (MQ=255)                                                                                                     gATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGa                                                >  3:52826/1‑149 (MQ=255)                                                                                                      aTGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCAGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGa                                                >  2:129548/1‑148 (MQ=255)                                                                                                            aTGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACAcccc                                          >  4:123783/1‑148 (MQ=255)                                                                                                                  gcgGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGtgtg                                   <  5:157448/149‑1 (MQ=255)                                                                                                                   cgGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTgg                                  <  7:163761/149‑1 (MQ=255)                                                                                                                   cgGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTgg                                  >  2:154268/1‑149 (MQ=255)                                                                                                                        tCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCttt                                                     <  5:108977/125‑1 (MQ=255)                                                                                                                        tCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCttt                                                     >  6:108977/1‑125 (MQ=255)                                                                                                                            cccTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGgc                         <  2:91201/149‑1 (MQ=255)                                                                                                                              cTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGgcgc                       <  3:194610/149‑1 (MQ=255)                                                                                                                               ttGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGtgtg                                   <  7:46743/136‑1 (MQ=255)                                                                                                                               ttGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTATGGCGACACCCCGAGtgtg                                   >  8:46743/1‑136 (MQ=255)                                                                                                                                    cTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTgg                                                                         <  7:45410/93‑1 (MQ=255)                                                                                                                                    cTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTgg                                                                         >  8:45410/1‑93 (MQ=255)                                                                                                                                     ttACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTggg                >  1:60859/1‑149 (MQ=255)                                                                                                                                     ttACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTggg                >  8:351100/1‑149 (MQ=255)                                                                                                                                     ttACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTggg                <  7:136454/149‑1 (MQ=255)                                                                                                                                     tcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTggg                >  5:173055/3‑149 (MQ=255)                                                                                                                                         ttGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAAc            >  6:162816/1‑149 (MQ=255)                                                                                                                                            cGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGACGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACCACCTGGCCGAGTGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGt          <  4:286008/148‑1 (MQ=255)                                                                                                                                                   tGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGa  >  3:151362/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                      CCGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGA  >  NC_002947/262606‑262900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑