Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,502,985	A→T	7.7%	S94C (AGC→TGC)	speB →	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,502,985	0	A	T	7.7%	188.0 / 5.5	65	S94C (AGC→TGC)	speB	agmatinase
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base T (3/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

CCACCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCC  >  NC_002947/2502839‑2503126                                                                                                                                                   |                                                                                                                                              ccaccaGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGc                                                                                                                                             >  7:155523/1‑149 (MQ=255)  caccaGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCg                                                                                                                                            <  2:243630/149‑1 (MQ=255)       gTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcg                                                                                                                                       >  7:43111/1‑149 (MQ=255)           ccGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgccg                                                                                                                                    >  2:378499/1‑148 (MQ=255)            cGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCa                                                                                                                                  <  1:48693/149‑1 (MQ=255)             gCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAg                                                                                                                                 >  1:157590/1‑149 (MQ=255)              ccccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGt                                                                                                                                <  8:155523/149‑1 (MQ=255)               cccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGCCTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTc                                                                                                                               >  5:225954/1‑149 (MQ=255)                ccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGTACGCCGCAGTCg                                                                                                                              <  7:319724/149‑1 (MQ=255)                ccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                              <  6:254505/149‑1 (MQ=255)                     gcgcgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCTACCCGTTCGACCACCTGGCGGTCATCGACTTTGGCGACTGTCCCTTCGACAGCGGCGTGCCGCTGTCgaaggg                                                                                                                         <  2:365183/149‑6 (MQ=255)                           ttCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGAc                                                                                                                     <  1:33261/147‑1 (MQ=255)                           ttCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGAc                                                                                                                     >  2:33261/1‑147 (MQ=255)                               gCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGACCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACATGGCGGTCATCGACATTGGCGACTGTCGCTTCGACAGCGGCACGCCGCAGTCGGTGCCGGACAGCAtg                                                                                                               >  2:380718/1‑148 (MQ=255)                                cccGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATc                                                                                                               >  5:228715/1‑148 (MQ=255)                                   gcgGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAgg                                                                                                           <  2:221216/149‑1 (MQ=255)                                         gATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATg                                                                                                     >  3:361037/1‑149 (MQ=255)                                           tCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATTGAGGCCCATGcc                                                                                                   >  8:304063/1‑149 (MQ=255)                                           tCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGcc                                                                                                   >  2:232000/1‑149 (MQ=255)                                               tgctgcCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCgaag                                                                                                                           >  6:69169/1‑118 (MQ=255)                                               tgctgcCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCgaag                                                                                                                           <  5:69169/121‑4 (MQ=255)                                                     cTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTc                                                                                         <  3:297631/149‑1 (MQ=255)                                                     cTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTc                                                                                         >  7:223459/1‑149 (MQ=255)                                                          tCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAg                                                                                    <  1:232000/149‑1 (MQ=255)                                                             agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                              >  8:117/1‑104 (MQ=255)                                                             agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                              <  7:117/104‑1 (MQ=255)                                                             agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAg                                                                                                <  4:327468/134‑1 (MQ=255)                                                             agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAg                                                                                                >  3:327468/1‑134 (MQ=255)                                                              gcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                >  8:264614/1‑149 (MQ=255)                                                              gcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                <  2:157590/149‑1 (MQ=255)                                                               cagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCggg                                                                               <  8:43111/149‑1 (MQ=255)                                                                agGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGt                                                                              <  4:361037/149‑1 (MQ=255)                                                                  gCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGCCAGCATCGAGGCCCATGCCGAGCACCTTCTGCAGGCGGGTTg                                                                            >  7:328800/1‑149 (MQ=255)                                                                  gCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTg                                                                            >  3:176539/1‑149 (MQ=255)                                                                    cTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGca                                                                                              >  6:364971/1‑129 (MQ=255)                                                                    cTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGca                                                                                              <  5:364971/129‑1 (MQ=255)                                                                            gCCACTGGCCGTGGGCGTTCGACCCGTTCGCCCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTg                                                                  >  3:234193/1‑149 (MQ=255)                                                                              cACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAc                                                                <  6:110163/149‑1 (MQ=255)                                                                                    ccGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgg                                                          <  2:307355/149‑1 (MQ=255)                                                                                       tGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtggtgg                                                       <  5:2173/149‑1 (MQ=255)                                                                                          gCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGa                                                    >  1:117368/1‑149 (MQ=255)                                                                                             ttcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCa                                                 >  2:301261/1‑149 (MQ=255)                                                                                               cgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCAc                                                <  6:228715/148‑1 (MQ=255)                                                                                                gacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACtt                                              <  1:187074/149‑1 (MQ=255)                                                                                                 acccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCAc                                                <  1:175552/146‑1 (MQ=255)                                                                                                 acccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCAc                                                >  2:175552/1‑146 (MQ=255)                                                                                                 acccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtc                                             <  7:264614/149‑1 (MQ=255)                                                                                                   ccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcat                                           <  8:223459/149‑1 (MQ=255)                                                                                                     gttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAAGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcat                                           >  5:197783/1‑147 (MQ=255)                                                                                                        cgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGc                                       <  1:301261/148‑1 (MQ=255)                                                                                                              ccTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCgc                                >  2:164315/1‑149 (MQ=255)                                                                                                                tGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCgctg                              >  8:240945/1‑149 (MQ=255)                                                                                                                    ggTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTc                           >  7:115070/1‑148 (MQ=255)                                                                                                                             cTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcg                 >  1:225037/1‑149 (MQ=255)                                                                                                                                tGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCTcgc                <  4:176539/147‑1 (MQ=255)                                                                                                                                   cGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGGATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcgcccgcc           >  1:266088/1‑149 (MQ=255)                                                                                                                                    gACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGAc                                                   <  6:44995/108‑1 (MQ=255)                                                                                                                                    gACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGAc                                                   >  5:44995/1‑108 (MQ=255)                                                                                                                                         tCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGCCCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg     >  6:364455/1‑149 (MQ=255)                                                                                                                                         tCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg     <  7:240945/149‑1 (MQ=255)                                                                                                                                          ggtaccGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATgg    >  6:324414/6‑149 (MQ=255)                                                                                                                                           ccTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGc   >  1:346465/1‑149 (MQ=255)                                                                                                                                            cTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGcc  <  6:225954/149‑1 (MQ=255)                                                                                                                                                gACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtc                                             >  4:269480/1‑102 (MQ=255)                                                                                                                                                gACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtc                                             <  3:269480/102‑1 (MQ=255)                                                                                                                                                   aGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCa       <  1:223063/137‑1 (MQ=255)                                                                                                                                                   aGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCa       >  2:223063/1‑137 (MQ=255)                                                                                                                                                   |                                                                                                                                              CCACCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCC  >  NC_002947/2502839‑2503126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑