Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,502,991 A→G 7.2% T96A (ACG→GCG) ‡ speB → agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,502,9910AG 7.2% 193.2 / 5.0 69T96A (ACG→GCG) ‡speBagmatinase
Reads supporting (aligned to +/- strand):  ref base A (33/31);  new base G (2/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGAT  >  NC_002947/2502845‑2503139
                                                                                                                                                  |                                                                                                                                                    
gTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcg                                                                                                                                                    >  7:43111/1‑149 (MQ=255)
    ccGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgccg                                                                                                                                                 >  2:378499/1‑148 (MQ=255)
     cGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCa                                                                                                                                               <  1:48693/149‑1 (MQ=255)
      gCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAg                                                                                                                                              >  1:157590/1‑149 (MQ=255)
       ccccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGt                                                                                                                                             <  8:155523/149‑1 (MQ=255)
        cccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGCCTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTc                                                                                                                                            >  5:225954/1‑149 (MQ=255)
         ccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGTACGCCGCAGTCg                                                                                                                                           <  7:319724/149‑1 (MQ=255)
         ccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                                           <  6:254505/149‑1 (MQ=255)
              gcgcgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCTACCCGTTCGACCACCTGGCGGTCATCGACTTTGGCGACTGTCCCTTCGACAGCGGCGTGCCGCTGTCgaaggg                                                                                                                                      <  2:365183/149‑6 (MQ=255)
                    ttCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGAc                                                                                                                                  <  1:33261/147‑1 (MQ=255)
                    ttCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGAc                                                                                                                                  >  2:33261/1‑147 (MQ=255)
                        gCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGACCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACATGGCGGTCATCGACATTGGCGACTGTCGCTTCGACAGCGGCACGCCGCAGTCGGTGCCGGACAGCAtg                                                                                                                            >  2:380718/1‑148 (MQ=255)
                         cccGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATc                                                                                                                            >  5:228715/1‑148 (MQ=255)
                            gcgGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAgg                                                                                                                        <  2:221216/149‑1 (MQ=255)
                                  gATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATg                                                                                                                  >  3:361037/1‑149 (MQ=255)
                                    tCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATTGAGGCCCATGcc                                                                                                                >  8:304063/1‑149 (MQ=255)
                                    tCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGcc                                                                                                                >  2:232000/1‑149 (MQ=255)
                                        tgctgcCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCgaag                                                                                                                                        <  5:69169/121‑4 (MQ=255)
                                        tgctgcCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCgaag                                                                                                                                        >  6:69169/1‑118 (MQ=255)
                                              cTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTc                                                                                                      <  3:297631/149‑1 (MQ=255)
                                              cTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTc                                                                                                      >  7:223459/1‑149 (MQ=255)
                                                   tCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAg                                                                                                 <  1:232000/149‑1 (MQ=255)
                                                      agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                                           >  8:117/1‑104 (MQ=255)
                                                      agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                                           <  7:117/104‑1 (MQ=255)
                                                      agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAg                                                                                                             <  4:327468/134‑1 (MQ=255)
                                                      agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAg                                                                                                             >  3:327468/1‑134 (MQ=255)
                                                       gcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                             >  8:264614/1‑149 (MQ=255)
                                                       gcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                             <  2:157590/149‑1 (MQ=255)
                                                        cagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCggg                                                                                            <  8:43111/149‑1 (MQ=255)
                                                         agGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGt                                                                                           <  4:361037/149‑1 (MQ=255)
                                                           gCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGCCAGCATCGAGGCCCATGCCGAGCACCTTCTGCAGGCGGGTTg                                                                                         >  7:328800/1‑149 (MQ=255)
                                                           gCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTg                                                                                         >  3:176539/1‑149 (MQ=255)
                                                             cTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGca                                                                                                           >  6:364971/1‑129 (MQ=255)
                                                             cTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGca                                                                                                           <  5:364971/129‑1 (MQ=255)
                                                                     gCCACTGGCCGTGGGCGTTCGACCCGTTCGCCCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTg                                                                               >  3:234193/1‑149 (MQ=255)
                                                                       cACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAc                                                                             <  6:110163/149‑1 (MQ=255)
                                                                             ccGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgg                                                                       <  2:307355/149‑1 (MQ=255)
                                                                                tGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtggtgg                                                                    <  5:2173/149‑1 (MQ=255)
                                                                                   gCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGa                                                                 >  1:117368/1‑149 (MQ=255)
                                                                                      ttcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCa                                                              >  2:301261/1‑149 (MQ=255)
                                                                                        cgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCAc                                                             <  6:228715/148‑1 (MQ=255)
                                                                                         gacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACtt                                                           <  1:187074/149‑1 (MQ=255)
                                                                                          acccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCAc                                                             >  2:175552/1‑146 (MQ=255)
                                                                                          acccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCAc                                                             <  1:175552/146‑1 (MQ=255)
                                                                                          acccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtc                                                          <  7:264614/149‑1 (MQ=255)
                                                                                            ccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcat                                                        <  8:223459/149‑1 (MQ=255)
                                                                                              gttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAAGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcat                                                        >  5:197783/1‑147 (MQ=255)
                                                                                                 cgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGc                                                    <  1:301261/148‑1 (MQ=255)
                                                                                                       ccTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCgc                                             >  2:164315/1‑149 (MQ=255)
                                                                                                         tGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCgctg                                           >  8:240945/1‑149 (MQ=255)
                                                                                                             ggTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTc                                        >  7:115070/1‑148 (MQ=255)
                                                                                                                      cTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcg                              >  1:225037/1‑149 (MQ=255)
                                                                                                                         tGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCTcgc                             <  4:176539/147‑1 (MQ=255)
                                                                                                                            cGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGGATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcgcccgcc                        >  1:266088/1‑149 (MQ=255)
                                                                                                                             gACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGAc                                                                <  6:44995/108‑1 (MQ=255)
                                                                                                                             gACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGAc                                                                >  5:44995/1‑108 (MQ=255)
                                                                                                                                  tCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGCCCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg                  >  6:364455/1‑149 (MQ=255)
                                                                                                                                  tCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg                  <  7:240945/149‑1 (MQ=255)
                                                                                                                                   ggtaccGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATgg                 >  6:324414/6‑149 (MQ=255)
                                                                                                                                    ccTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGc                >  1:346465/1‑149 (MQ=255)
                                                                                                                                     cTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGcc               <  6:225954/149‑1 (MQ=255)
                                                                                                                                         gACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtc                                                          <  3:269480/102‑1 (MQ=255)
                                                                                                                                         gACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtc                                                          >  4:269480/1‑102 (MQ=255)
                                                                                                                                            aGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCa                    <  1:223063/137‑1 (MQ=255)
                                                                                                                                            aGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCa                    >  2:223063/1‑137 (MQ=255)
                                                                                                                                              cGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgcgc      <  2:346465/149‑1 (MQ=255)
                                                                                                                                              cGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCACGGCCCGCTGgcgc      >  4:271040/1‑149 (MQ=255)
                                                                                                                                              cGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGGTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgcgc      <  5:364455/149‑1 (MQ=255)
                                                                                                                                                 cACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGa   <  1:378499/149‑1 (MQ=255)
                                                                                                                                                  aCGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGAt  >  4:352082/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGAT  >  NC_002947/2502845‑2503139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑