Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,505,438 T→C 7.3% L13P (CTG→CCG)  PP_2199 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,505,4380TC 7.3% 194.2 / 4.7 68L13P (CTG→CCG) PP_2199hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base C (3/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

ATGGTTACCTGTATGTACTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTGGCTGATG  >  NC_002947/2505292‑2505583
                                                                                                                                                  |                                                                                                                                                 
aTGGTTACCTGTATGTACTGACCGATGACAAGGTTGGCGCGCTACTCAAGGTCGGCCTGTCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGa                                                                                                                                                 <  8:310059/149‑1 (MQ=255)
 tGGTTACCTGTATGTACTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGa                                                                                                                                                 <  2:378520/148‑1 (MQ=255)
 tGGTTACCGCTATGTACTGCCCTATGACAAGGATGGCTCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTTCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGa                                                                                                                                                 <  8:93582/148‑1 (MQ=255)
   gTTACCTGTATGTACTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCCGAgtg                                                                                                                                              >  4:122072/1‑147 (MQ=255)
    ttACCTGTATGTACTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCa                                                                                                                                              >  6:24240/1‑148 (MQ=255)
      aCCTGTATGTACTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTc                                                                                                                                           >  8:273685/1‑149 (MQ=255)
         tgtatgtaCTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCggg                                                                                                                                        >  5:83209/1‑149 (MQ=255)
              gtaCTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTAc                                                                                                                                   >  3:151770/1‑149 (MQ=255)
               taCTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCCGAGTGCTCAGGGCttgc                                                                                                                                  <  3:122072/149‑4 (MQ=255)
                   gACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGc                                                                                                                              >  5:169630/1‑149 (MQ=255)
                   gACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGc                                                                                                                              >  3:11505/1‑149 (MQ=255)
                      cGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGa                                                                                                                           >  3:189517/1‑149 (MQ=255)
                      cGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCCGAGTGCTCAGGGCttgc                                                                                                                                  <  6:177231/142‑4 (MQ=255)
                      cGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCCGAGTGCTCAGGGCttgc                                                                                                                                  >  5:177231/1‑139 (MQ=255)
                       gATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGAc                                                                                                                          >  2:375808/1‑149 (MQ=255)
                        aTGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGAcc                                                                                                                         <  6:136560/149‑1 (MQ=255)
                           aCAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCCAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTg                                                                                                                      >  7:63403/1‑149 (MQ=255)
                              aGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGcc                                                                                                                    >  2:81329/1‑148 (MQ=255)
                               ggATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGcc                                                                                                                    >  5:352474/1‑147 (MQ=255)
                                gATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAg                                                                                                                 >  7:327959/1‑149 (MQ=255)
                                  tGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGGCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGc                                                                                                               >  5:246352/1‑149 (MQ=255)
                                    gcgcgcTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGccc                                                                                                             >  5:23639/1‑149 (MQ=255)
                                      gcgcTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCcgc                                                                                                           >  7:70349/1‑149 (MQ=255)
                                            cTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCgtcgtc                                                                                                     >  3:276521/1‑149 (MQ=255)
                                                cagTCGGCCAGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAgg                                                                                                 >  3:175031/3‑149 (MQ=255)
                                                  gTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGcgtc                                                                                                        <  1:315516/140‑1 (MQ=255)
                                                  gTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGcgtc                                                                                                        >  2:315516/1‑140 (MQ=255)
                                                    cGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCtt                                                                                             <  4:276521/149‑1 (MQ=255)
                                                     ggCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTg                                                                                            <  6:23639/149‑1 (MQ=255)
                                                       ccTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGCGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGc                                                                                           >  6:81515/1‑148 (MQ=255)
                                                              gACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGc                                                                                   <  5:24240/149‑1 (MQ=255)
                                                               aCTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGG‑CTTGCAAggcctcga                                                                                 <  6:83209/149‑8 (MQ=255)
                                                                 tGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTc                                                                                >  6:152625/1‑149 (MQ=255)
                                                                 tGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTc                                                                                >  6:117881/1‑149 (MQ=255)
                                                                        cgcCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTccc                                                                         <  5:117881/149‑1 (MQ=255)
                                                                                ccGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAg                                                                 <  6:24656/149‑1 (MQ=255)
                                                                                 cGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAgg                                                                <  1:375808/149‑1 (MQ=255)
                                                                                    gggTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGgcc                                                             <  7:273685/149‑1 (MQ=255)
                                                                                       tGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGgccgcc                                                          >  2:223197/1‑149 (MQ=255)
                                                                                          cAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCccca                                                       >  6:238225/1‑149 (MQ=255)
                                                                                           aGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCcccac                                                      >  3:366646/1‑149 (MQ=255)
                                                                                            gTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCcccacc                                                     <  6:246352/149‑1 (MQ=255)
                                                                                                  tAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGc                                               <  4:175031/149‑1 (MQ=255)
                                                                                                     aaTGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCct                                             >  8:48971/1‑148 (MQ=255)
                                                                                                      aTGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCACGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCctct                                           <  3:44034/149‑1 (MQ=255)
                                                                                                             aTGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTg                                    <  1:81329/149‑1 (MQ=255)
                                                                                                                  ccTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGcc                               >  2:159159/1‑149 (MQ=255)
                                                                                                                  ccTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGcc                               <  5:84815/149‑1 (MQ=255)
                                                                                                                  ccTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGcc                               >  6:84815/1‑149 (MQ=255)
                                                                                                                   cTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTTCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCATTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGccc                              >  5:21662/1‑149 (MQ=255)
                                                                                                                       aCCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTc                          <  5:152625/149‑1 (MQ=255)
                                                                                                                       aCCCCCAAACCCGCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTc                          <  8:63403/149‑1 (MQ=255)
                                                                                                                         ccccAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCgg                        <  8:327959/149‑1 (MQ=255)
                                                                                                                         ccccAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCgg                        <  4:189517/149‑1 (MQ=255)
                                                                                                                          cccAAACCCTCTACCAGCACGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCggg                       <  5:13304/149‑1 (MQ=255)
                                                                                                                           ccAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTc                          >  7:14938/1‑145 (MQ=255)
                                                                                                                           ccAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTc                          <  8:14938/145‑1 (MQ=255)
                                                                                                                              aaCCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGcgcg                   >  7:45710/1‑149 (MQ=255)
                                                                                                                               aCCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGTCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCgg                  >  5:152915/1‑149 (MQ=255)
                                                                                                                                cccTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGc                 <  1:229987/149‑1 (MQ=255)
                                                                                                                                     tACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGAc            >  1:149352/1‑149 (MQ=255)
                                                                                                                                       ccAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGAc            <  8:45710/147‑1 (MQ=255)
                                                                                                                                         aGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTgg        <  4:11505/149‑1 (MQ=255)
                                                                                                                                          gCAAGCACTGAGCACTCGGGGCTAAGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGAGTGCTTCCAGTCCCTTGAGCACGGCCGCCCCACCCAGGGGCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGAACTGGc       >  4:365897/1‑149 (MQ=255)
                                                                                                                                          cgtaGCCCCGAGTGCTCAGGGCTACGTTGCAGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGACCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTGGc       >  1:31179/4‑149 (MQ=255)
                                                                                                                                               cccTGAGCACTCGGGGCTACGTTGCTGTCCATGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCTCCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTGGCTGATg  <  6:169630/149‑1 (MQ=255)
                                                                                                                                               cccTGAGCACTCGGGGCTACGTTGCTGACCCTTCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTAAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTGGCTGATg  <  8:70349/149‑1 (MQ=255)
                                                                                                                                               cccTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGACCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTGGCTGATg  >  1:42979/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGGTTACCTGTATGTACTGACCGATGACAAGGATGGCGCGCTACTCAAGGTCGGCCTGGCCGACTGACGCGCGCCTGCACCGAGGGTGACAGTCGGGTAGAATGCGGCATGGCCCTCGACCCCCAAACCCTCTACCAGCAAGCCCTGAGCACTCGGGGCTACGTTGCTGACCCTGCCCAGGCCCGCGTCGTCGAGGCCTTGCAAGCCTGCTTCCAGTCCCTTGAGCAGGGCCGCCCCACCCAGGGCCTCTACCTCTGGGGCCCGGTCGGGCGCGGCAAGACCTGGCTGATG  >  NC_002947/2505292‑2505583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑