Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,571,106	G→A	12.1%	intergenic (‑24/+20)	PP_2254 ← / ← dctA‑III	GntR family transcriptional regulator/C4‑dicarboxylate transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,571,106	0	G	A	12.1%	122.6 / 5.0	49	intergenic (‑24/+20)	PP_2254/dctA‑III	GntR family transcriptional regulator/C4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base G (25/18);  new base A (3/3);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TGCCCAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCA  >  NC_002947/2570959‑2571247                                                                                                                                                      |                                                                                                                                               tGCCCAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGt                                                                                                                                               >  6:295441/1‑149 (MQ=255)  gCCCAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGt                                                                                                                                               >  7:4039/1‑148 (MQ=255)     cAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGGTGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑Tg                                                                                                                                          >  3:276629/1‑149 (MQ=255)        ttgttCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCTCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCg                                                                                                                                       <  6:230452/149‑1 (MQ=255)               gCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGACGGCGTCA‑TGCCGGCCACGt                                                                                                                                >  8:130687/1‑149 (MQ=255)                ccAGTTCGCGCTGGCCTGGCAGCAGGGCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCATGA‑CGCCGGcccttcc                                                                                                                               <  7:348769/149‑6 (MQ=255)                 cAGTTGGCGGTGGCGTGGCAGCATGTGGGGGGTGGGCGATTGGCGGGGGGCTTGGGCTTGGGGGAGTTTTTCCACCATTGTG‑TTGA‑CGAGGGTTTATGAGCTGATCAGGTTTCGCTCTTGGAAGGGCGGGCGTCA‑TGCCGGCCACGTTc                                                                                                                              <  8:290444/149‑1 (MQ=255)                 cAGGTCGCGCGGGCCTGCCAGCATGGCGCCGGTGCGCCATGGGCCGCGGGCCTGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTCATCACGGTTCGCTCCTGGAAGGGCCGGCATGA‑CGCCGGcccttcca                                                                                                                              <  6:231849/149‑7 (MQ=255)                  aGTTCGCGCGGGCCGGGCCGCATGTCGCGGGTGCGCCATTGGCCGCGGGCCTGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTCA‑CGCCGTTTGTTGCGCTGATCCCGGTGCGCCCCTGTACGGGCCGGCGTCA‑TGCCGGCCACGTTCg                                                                                                                             <  6:127739/149‑1 (MQ=255)                    tgcgcgCTGGCCGGGCAGCATGGGGCCGGTGCGCCTTTGGCCGCGGGCATGGGCCGGGCGTAGTTTTTCCACCCCTGAG‑TTGC‑CGACGGTTGATGCGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCt                                                                                                                           <  8:183328/147‑1 (MQ=255)                               ctggcAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTg                                                                                                                >  7:22348/1‑149 (MQ=255)                                tggcAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGt                                                                                                               >  1:342368/1‑149 (MQ=255)                                    agcaTGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGAt                                                                                                           >  2:345537/1‑149 (MQ=255)                                       aTGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATggg                                                                                                        <  1:231041/149‑1 (MQ=255)                                            gCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATGCCTGTTGATGGGCGCTg                                                                                                   >  7:191969/1‑149 (MQ=255)                                                          ggCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTgcgtg                                                                                      <  3:81992/148‑1 (MQ=255)                                                            ccGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTgcgtggcg                                                                                   >  1:281739/1‑149 (MQ=255)                                                            ccGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTgcgtggcg                                                                                   >  7:107547/1‑149 (MQ=255)                                                               cgGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGcgtggcgtgg                                                                                <  5:295441/149‑1 (MQ=255)                                                               cgGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGcgtggcgtgg                                                                                <  1:109917/149‑1 (MQ=255)                                                                gggCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTggg                                                                               >  6:119519/1‑149 (MQ=255)                                                                 ggCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTggg                                                                               >  1:241452/1‑149 (MQ=255)                                                                      gggCCGGGCGTTGTTTTTCCACCCCTGA‑‑TTGACCGACGGTGCCTGAGGTTATCACGGTTCGCTCGTGCATGGGCCGGCGTCA‑TGCGGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCaaa                                                                         <  7:130687/149‑1 (MQ=255)                                                                           tGGCGGAGTTTTTCCCCCCCTGCG‑TTGC‑CGCCGGTGCATGCGCTGATCCCGGTGCGCTCCTGGACGGGCCGGCGTCT‑TGCGGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAg                                                                    <  1:138513/149‑1 (MQ=255)                                                                           tGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAg                                                                    >  5:351683/1‑149 (MQ=255)                                                                             gCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTGCTGTTGATGGGCGCTGGGTTCGTTGCGTGGCTTGGGGGCAAAGCCAGGc                                                                  >  5:217224/1‑149 (MQ=255)                                                                             gCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGc                                                                  <  2:241452/149‑1 (MQ=255)                                                                                 aGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTTATGAGCTGATCACGTTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGc                                                              <  2:209018/149‑1 (MQ=255)                                                                                     tttCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTc                                                          >  7:26980/1‑149 (MQ=255)                                                                                            cacTGAG‑TTTA‑CGACGGTTTATTAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACg                                                   <  2:281739/149‑1 (MQ=255)                                                                                                 cg‑TTGA‑CGACGGTTGCTGAGCTGATCACGGTTCGCTCCTGGAAGGGCGGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCt                                               <  6:346826/147‑1 (MQ=255)                                                                                                      ga‑cgacGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAg                                                                                                                        >  3:24602/1‑71 (MQ=255)                                                                                                      ga‑cgacGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGttgctt                                                                                           >  1:366158/1‑100 (MQ=255)                                                                                                            cgGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGttg                                     >  5:49145/1‑148 (MQ=255)                                                                                                                   tgaGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTc                               >  6:213995/1‑148 (MQ=255)                                                                                                                     aGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAAt                            <  5:213995/149‑1 (MQ=255)                                                                                                                      gCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATg                           >  1:53824/1‑149 (MQ=255)                                                                                                                         gctcccGGTGCGCCCCTGGACGGG‑CGGCGGCACTGCGGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTcc                        <  2:342368/144‑1 (MQ=255)                                                                                                                         gATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTcc                        >  5:49217/1‑149 (MQ=255)                                                                                                                          aTCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑AGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGTTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCt                       >  2:75349/1‑149 (MQ=255)                                                                                                                             aCGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTtct                    >  4:95644/1‑149 (MQ=255)                                                                                                                               ggTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTcc                  >  7:326188/1‑149 (MQ=255)                                                                                                                                     cTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACgg            <  8:22348/149‑1 (MQ=255)                                                                                                                                          aagggggCCGGCATGA‑CGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTtct                    <  1:148762/132‑1 (MQ=255)                                                                                                                                            aaGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATgg     <  8:107547/149‑1 (MQ=255)                                                                                                                                              tggCCGGCATGA‑CGCCGGCCACGTTCGCTCAGAATACCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCGCCCCACGGGCAATGGc    >  1:205661/2‑148 (MQ=255)                                                                                                                                               ggCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCa  >  5:350371/1‑149 (MQ=255)                                                                                                                                               ggCCGGCATGA‑CGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCa  >  6:383639/1‑149 (MQ=255)                                                                                                                                               ggCCGGCATGA‑CGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCa  >  6:226413/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                               TGCCCAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAG‑TTGA‑CGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCA‑TGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCA  >  NC_002947/2570959‑2571247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑