Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,607,464 G→A 8.1% R658R (CGG→CGA)  PP_2284 → tail tubular protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,607,4640GA 8.1% 139.2 / 2.4 49R658R (CGG→CGA) PP_2284tail tubular protein B
Reads supporting (aligned to +/- strand):  ref base G (27/18);  new base A (2/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

GTGGGCTATGGATGACGGCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGTACTTCCAGGTGAAG  >  NC_002947/2607317‑2607605
                                                                                                                                                   |                                                                                                                                             
gTGGGCTATGGATGACGGCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTcggcgg                                                                                                                                               <  7:170941/148‑1 (MQ=255)
             gACGGCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACATCCTCGGACCTAATCGTCGGTCGGCGGTAAGTGTTCATCt                                                                                                                                  >  6:290811/1‑148 (MQ=255)
              aCGGCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTc                                                                                                                                <  4:51397/149‑1 (MQ=255)
                ggCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTa                                                                                                                                             <  8:241475/134‑1 (MQ=255)
                ggCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTa                                                                                                                                             >  7:241475/1‑134 (MQ=255)
                     gTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCt                                                                                                                                  >  4:178050/1‑140 (MQ=255)
                     gTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCt                                                                                                                                  <  3:178050/140‑1 (MQ=255)
                        cATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGGCGGCGGTACGTGTTCAGCTTCCAGCTAAGc                                                                                                                       <  5:290811/148‑1 (MQ=255)
                              cGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGATACGTGTTCAGCTTCCAGCTAAGCACCATCa                                                                                                                >  5:281568/1‑149 (MQ=255)
                              cGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGATACGTGTTCAGCTTCCAGCTAAGCACCATCa                                                                                                                >  3:288613/1‑149 (MQ=255)
                                      ccACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTa                                                                                                           >  6:199554/1‑146 (MQ=255)
                                       cACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGa                                                                                                       >  4:259184/1‑149 (MQ=255)
                                         ccGGGCAGACCCTCAAGCCTGGCGTGTTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGAcc                                                                                                     <  4:224161/149‑1 (MQ=255)
                                          cGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGATACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGAccc                                                                                                    <  6:281568/149‑1 (MQ=255)
                                          cGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGATACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGAccc                                                                                                    <  4:288613/149‑1 (MQ=255)
                                                     tCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGTTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGc                                                                                         >  7:173436/1‑149 (MQ=255)
                                                         gCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAATGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCgggg                                                                                     <  7:342125/149‑1 (MQ=255)
                                                            tGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACATCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCAACATCACCCTAAAGACCAAAAGCACAGGCGGGGGCa                                                                                  >  4:225249/1‑149 (MQ=255)
                                                                   gTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAagag                                                                           >  4:12076/1‑149 (MQ=255)
                                                                             ttGTGTGGGATGGGACAACAGCGAAGGTGAAGTGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGaa                                                                 <  5:199554/149‑1 (MQ=255)
                                                                                 gtgGGATGGGACAACAGCGAAGGAGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGCTCGGCGGTAAGTGTTCACCTTCCAGCTAAGCACAATCACCGAAAAGACCCAAAGCGCAGGCCGGTGCACCAAGACCGACAACGaacgac                                                             >  2:248857/1‑145 (MQ=255)
                                                                                  tgggatgggaCAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGgccg                                                            >  1:100723/1‑149 (MQ=255)
                                                                                      atgggaCAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTc                                                        >  2:190685/1‑149 (MQ=255)
                                                                                       tgggaCAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTc                                                        >  8:52484/1‑148 (MQ=255)
                                                                                          gaCAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGc                                                    >  5:242238/1‑149 (MQ=255)
                                                                                          gaCAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCCGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCacc                                                    >  5:295924/1‑147 (MQ=255)
                                                                                            caacaGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCtt                                                  <  5:166134/149‑1 (MQ=255)
                                                                                             aacaGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTc                                                 <  2:100723/149‑1 (MQ=255)
                                                                                               caGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCa                                                                                                                                     >  6:267390/1‑63 (MQ=255)
                                                                                               caGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCa                                                                                                                                     <  5:267390/63‑1 (MQ=255)
                                                                                                  cGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAg                                            <  3:140527/149‑1 (MQ=255)
                                                                                                  cGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATAGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAg                                            >  6:236803/1‑149 (MQ=255)
                                                                                                   gAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAgg                                           >  1:157026/1‑149 (MQ=255)
                                                                                                       gTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTc                                       >  7:141245/1‑149 (MQ=255)
                                                                                                       gTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTc                                       >  7:93228/1‑149 (MQ=255)
                                                                                                        ttattgggAATTACACCTGCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGTTAAGCACCATGTACGTAAAGTCGCAAAGCGCAGGCGTGGGCACCAAGAGCGACACCGAAGGCCGACTCCAGCTTCGCAAGGCGTcc                                      <  8:173436/144‑1 (MQ=255)
                                                                                                            ggggAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTg                                   >  6:72890/1‑148 (MQ=255)
                                                                                                                 aTTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACttt                             >  6:69371/1‑149 (MQ=255)
                                                                                                                        ctcctcGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCa                      >  5:375512/1‑149 (MQ=255)
                                                                                                                        ctcctcGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGACCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCa                      >  3:208294/1‑149 (MQ=255)
                                                                                                                            tcGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTc                                       <  7:11208/128‑1 (MQ=255)
                                                                                                                            tcGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTc                                       >  8:11208/1‑128 (MQ=255)
                                                                                                                              ggACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGAGCGAAAGCGGAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCgg                  <  3:225249/147‑1 (MQ=255)
                                                                                                                              ggACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCggg                 <  3:259184/148‑1 (MQ=255)
                                                                                                                              ggACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCggg                 <  6:242238/148‑1 (MQ=255)
                                                                                                                              ggACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGt                <  3:12076/149‑1 (MQ=255)
                                                                                                                               gACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGTa               >  5:70690/1‑149 (MQ=255)
                                                                                                                                     aTCGTCGGTCGGCGGTACGTGTACAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGTACTTc           >  5:120750/1‑147 (MQ=255)
                                                                                                                                      tcgtcgGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGTACTTCCag        <  2:157026/149‑1 (MQ=255)
                                                                                                                                            gtcggCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGTACTTCCaggtgaag  >  4:124694/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GTGGGCTATGGATGACGGCACGTACATGGCCGTGACCGCCACCGGGCAGACCCTCAAGCCTGGCGTGGTCGCTGAAATTGTGTGGGATGGGACAACAGCGAAGGTGAAGGGGAATTACACCTCCTCGGACCTTATCGTCGGTCGGCGGTACGTGTTCAGCTTCCAGCTAAGCACCATCACCGTAAAGACCCAAAGCGCAGGCGGGGGCACCAAGAGCGACACCGAAGGCCGCCTCCAGCTTCGCAAGGCGTCCGTGAACTTTGCCAGCACCGGGTACTTCCAGGTGAAG  >  NC_002947/2607317‑2607605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑